To establish a HPLC method to determine the content of aucubin in Eucommia ulmoides seeds,to search for the formation and accumulation of aucubin in Eucommia ulmoides seeds,and to provide foundation for the suitable h...To establish a HPLC method to determine the content of aucubin in Eucommia ulmoides seeds,to search for the formation and accumulation of aucubin in Eucommia ulmoides seeds,and to provide foundation for the suitable harvest season of aucubin in Eucommia ulmoides seeds,the Hypersil GLOD-C18 column(250 mm×4.6 mm,5μm)with temperature at 25 ℃ was used.The mobile phase was methanol-water(8∶92),at a flow rate of 1.0 mL·min-1,and the detective wavelength was 206 nm.The results indicated that the method of HPLC was efficient,and the average recovery of the aucubin in Eucommia ulmoides seeds was 98.61%,RSD=1.14%(n=6).The determination of aucubin was gradually raised with the seed growth,while the formation and accumulation from June 25th to September 5th increased slowly,and the determination of aucubin from September 5th to 25th raised quickily,and then kept relatively stable.Considering the extraction and utilization of aucubin,the date from September 25th to October 25th was the most suitable period for collecting Eucommia ulmoides seeds.展开更多
文摘为探究蒸汽爆破(汽爆)辅助加工制成杜仲叶茶的挥发性成分差异和主要呈香特征,以杜仲‘华仲8号’嫩叶和成熟叶为对象,采用汽爆技术对杜仲叶进行处理,基于电子鼻和顶空固相微萃取-气相色谱-质谱(headspace solid-phase microextraction combined with gas chromatography-mass spectrometry,HS-SPME-GC-MS)联用法解析汽爆对两种杜仲茶香气特征的影响。主成分分析和线性判别分析对电子鼻数据模型拟合度高,杜仲嫩叶和成熟叶茶汽爆前后香气特征均存在显著差异。HS-SPME-GC-MS检测共鉴定出177种挥发性成分,通过正交偏最小二乘判别分析(变量重要性投影值≥1)以及Kruskal-WallisH检验(P<0.05)进一步筛选出24种香气物质,发现汽爆前嫩叶茶关键香气物质为二氢猕猴桃内酯。汽爆后嫩叶茶关键呈香物质在此基础上增加了壬醛、苯甲醛和苯乙醛,呈现柑橘香、花香、焦糖香、苦杏仁味、坚果香、玫瑰香和巧克力香。汽爆前成熟叶茶无关键香气物质,汽爆后成熟叶茶关键香气物质为二氢猕猴桃内酯和壬醛,呈现甜桃香、木香、柑橘香、花香和焦糖香。研究结果可为开发杜仲叶茶饮品提供参考依据。
文摘To establish a HPLC method to determine the content of aucubin in Eucommia ulmoides seeds,to search for the formation and accumulation of aucubin in Eucommia ulmoides seeds,and to provide foundation for the suitable harvest season of aucubin in Eucommia ulmoides seeds,the Hypersil GLOD-C18 column(250 mm×4.6 mm,5μm)with temperature at 25 ℃ was used.The mobile phase was methanol-water(8∶92),at a flow rate of 1.0 mL·min-1,and the detective wavelength was 206 nm.The results indicated that the method of HPLC was efficient,and the average recovery of the aucubin in Eucommia ulmoides seeds was 98.61%,RSD=1.14%(n=6).The determination of aucubin was gradually raised with the seed growth,while the formation and accumulation from June 25th to September 5th increased slowly,and the determination of aucubin from September 5th to 25th raised quickily,and then kept relatively stable.Considering the extraction and utilization of aucubin,the date from September 25th to October 25th was the most suitable period for collecting Eucommia ulmoides seeds.
