Looked back to the developed course of the university education of China concisely.Studied the source condition and tendency of students in two aspects of the high school developed level and the population condition.E...Looked back to the developed course of the university education of China concisely.Studied the source condition and tendency of students in two aspects of the high school developed level and the population condition.Exposed the latent crisis of the university education of China.Pointed out some erroneous opinions on the development of the university education of China.Suggested several steps to prevent the crisis.展开更多
目的在体研究切应力诱导内皮细胞TF基因表达变化规律及其机制。方法54只SD大鼠随机分为对照组和颈动脉狭窄组,狭窄组有又分为0.5、1、3、6、12 h、1、3、7 d 8个时相点,套扎法建立左颈总动脉狭窄模型,术后不同时相点用原位杂交和免疫组...目的在体研究切应力诱导内皮细胞TF基因表达变化规律及其机制。方法54只SD大鼠随机分为对照组和颈动脉狭窄组,狭窄组有又分为0.5、1、3、6、12 h、1、3、7 d 8个时相点,套扎法建立左颈总动脉狭窄模型,术后不同时相点用原位杂交和免疫组化法检测TF、Egr、Sp-1的mRNA和蛋白,用图像分析系统测定内膜平均灰度,进行统计学分析。结果正常对照组内皮细胞TF、Egr-1、Sp-1的mRNA和蛋白弱表达,狭窄30 m in后,内皮细胞胞浆组织因子基因mRNA转录和蛋白合成升高,与对照组比较均有显著性差异(P<0.05)。内皮细胞胞核和胞浆的Sp-1和Egr-1基因mRNA转录和蛋白合成均有显著性增加(P<0.05),但以Egr-1增加更显著(P<0.05),其变化趋势与组织因子基因mRNA转录、组织因子基因蛋白合成的变化趋势相同。TF mRNA和蛋白6 h达到峰值,Egr-1 mRNA和蛋白3 h达到峰值,Sp-1 mRNA和蛋白1 h达到峰值,与邻近组比较差异显著(P<0.05)。结论切应力是内皮细胞组织因子基因表达上调的触发因素之一,切应力激活TF与转录因子Egr-1和Sp-1介导有关。展开更多
文摘纵隔子宫(septate uterus)是最常见的女性生殖器畸形,但是临床医生常常被有关其诊断和治疗的一些问题所困惑。比如:纵隔子宫诊断的金标准是什么?纵隔子宫的宫底外形可能是不正常的吗?纵隔子宫的手术指征(特别是很浅的纵隔是否需要手术)?经宫颈子宫纵隔切除术(transcervical resection of septum,TCRS)术中采用何种监护方式最好?切除子宫纵隔时采用针状电极还是环状电极?TCRS术中何时可以认为纵隔已经完全切除了?TCRS术中发现子宫左右侧壁明显凸向宫腔是否需要同时矫形?TCRS术后是否需要放置宫内节育器或者用雌激素促进子宫内膜生长?TCRS术后为什么容易出现阴道出血量较多的现象?等等。这些问题直接影响了临床医生的临床决策,也造成了妇科和生殖科医生的认识混乱。针对纵隔子宫诊断和治疗相关的临床问题进行解析,以期进一步指导临床实践。
文摘Looked back to the developed course of the university education of China concisely.Studied the source condition and tendency of students in two aspects of the high school developed level and the population condition.Exposed the latent crisis of the university education of China.Pointed out some erroneous opinions on the development of the university education of China.Suggested several steps to prevent the crisis.
