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中孔分子筛SBA-15作为液相色谱固定相以及在巯基化合物分离中的应用 被引量:14
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作者 高峰 赵建伟 +5 位作者 张松 周峰 金婉 张祥民 杨原 赵东元 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2002年第8期1494-1497,共4页
采用共表面活性剂法合成了孔道结构高度有序、孔径较大且粒径均匀的 SBA-1 5颗粒 ,并在此基础上研究了其作为毛细管液相色谱柱的填料基体的特性 .在 SBA-1 5材料上进行 C1 8官能团化前后 ,分别对 4种巯基化合物即半胱氨酸、谷胱甘肽、... 采用共表面活性剂法合成了孔道结构高度有序、孔径较大且粒径均匀的 SBA-1 5颗粒 ,并在此基础上研究了其作为毛细管液相色谱柱的填料基体的特性 .在 SBA-1 5材料上进行 C1 8官能团化前后 ,分别对 4种巯基化合物即半胱氨酸、谷胱甘肽、多巴胺和 展开更多
关键词 中孔分子筛 SBA-15 高效液相色谱 固定相 巯基化合物 分离 分析
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用于蛋白质分析的毛细管等电聚焦-电喷雾质谱接口的改进 被引量:3
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作者 许崇峰 宋浩威 +3 位作者 杨原 王谦 王洪海 许云敏 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2002年第6期1035-1037,共3页
An important interface modification for capillary isoelectric focusing mass spectrometry(CIEF MS) is reported. A capillary needle with a sheath flow tube pre mounted in one device serves as both the CIEF cathodic end ... An important interface modification for capillary isoelectric focusing mass spectrometry(CIEF MS) is reported. A capillary needle with a sheath flow tube pre mounted in one device serves as both the CIEF cathodic end and the ESI interface. The needle was inserted into the cathode reservoir at the focusing stage, and then removed to the front of sampler orifice of MS at the detection stage. Therefore, the shut off of the high voltage is not necessary and a better focusing protein zone is maintained during the ESI stage. The optimization of the experimental condition has been investigated and the two dimensional image of the E. coli proteins has been obtained, which is comparable with that from a 2D gel. 展开更多
关键词 蛋白质 毛细管等电聚焦-质谱 接口 分离 鉴定 大肠杆菌 蛋白质组学
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离线2D-LC-MS系统的建立及其用于人肝蛋白质组学的研究 被引量:3
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作者 王静 许雪姣 +2 位作者 于雁灵 刘银坤 杨原 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2004年第12期2247-2249,共3页
A lab-made off line 2D-LC-MS system was developed for protein analysis. In case the off line 2D-LC was utilized for peptide separation, and the working conditions for strong cation exchange(SCX) chromatography and the... A lab-made off line 2D-LC-MS system was developed for protein analysis. In case the off line 2D-LC was utilized for peptide separation, and the working conditions for strong cation exchange(SCX) chromatography and the succeeding reversed phase(RP) chromatography can be individually optimized, contrasted to the on-line system. The experimental results show that the mass fractions of acetonitrile for the SCX separation can be increased form 5% as references reported up to 20% so that the separation of peptides has been substantially improved. The off line system has been applied to the analysis of proteins extracted from the cancer tissue of nude mice inoculated by a human-hepatocellular-carcinoma cell-line D20. Some extreme basic proteins(pI>10) and larger proteins(M w>200 000), which are difficult to be isolated by the traditional 2D-PAGE method, have been identified in D20 sample. 展开更多
关键词 多维色谱-质谱联用仪 离线2D-LC-MS 水溶性蛋白
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蛋白质酶解混合物的动态修饰毛细管电泳-质谱联用分析 被引量:1
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作者 王晓燕 牟晓盾 +2 位作者 许云敏 杨原 王洪海 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2004年第7期1226-1230,MJ02,共6页
利用羟丙基纤维素溶液动态涂层技术修饰毛细管管壁 ,改善了分离效率 .在不影响质谱检测的条件下 ,将动态涂层毛细管电泳与质谱检测联用 ,有效地提高了对蛋白质的鉴定能力 .将该技术应用于对复杂蛋白质样品的酶解产物的分析鉴定 。
关键词 蛋白质酶解混合物 鉴定 毛细管电泳-质谱联用技术 羟丙基纤维素溶液 动态涂层
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