Aging refers to a multidimensional process that all changes were accumulated in a person over time.These aging changes are associated with progressive increases in the chance of disease and death. Thus,it is necessary...Aging refers to a multidimensional process that all changes were accumulated in a person over time.These aging changes are associated with progressive increases in the chance of disease and death. Thus,it is necessary to establish a model to screen biomarkers to characterize and evaluate aging degree. In this study,an in vitro aging model was set up by formaldehdye and human serum albumin( HSA),the most abundant protein in human plasma,based on Maillard Reaction. The liquid chromatography tandem mass spectrometry( LC-MS /MS) method with ^(18)O-labeling technique was employed to quantify modification degree of peptides cleaved from HSA. This model was established and optimized for further quantitative biomarker study.展开更多
The CP4-EPSPS gene is widely used in herbicide-tolerant plants/crops all over the world.In this study,a method was developed by coupling liquid chromatography with high sensitivity to tandem mass spectrometry to quant...The CP4-EPSPS gene is widely used in herbicide-tolerant plants/crops all over the world.In this study,a method was developed by coupling liquid chromatography with high sensitivity to tandem mass spectrometry to quantify the amount of CP4-EPSPS expression in Nicotiana tabacum leaves.The quantification of protein was converted to measure the unique peptide of CP4-EPSPS protein.One peptide unique to CP4-EPSPS was synthesized and labeled with H18 8 2O to get 1O stable isotope labeled peptide.The peptide served as the internal standard.The validated method had good specificity and linearity.The intra-and inter-day precisions and accuracy for all samples were satisfactory.The results demonstrated that the novel method was sensitive and selective to quantify CP4-EPSPS in the crude extract without time-consuming pre-separation or the purification procedures.展开更多
基金This work was supported by the National Natural Science Fundation for Distinguished Young Scholars in China (No. 30425024)the National Basic Research Development Program of China (973 Program) (No. 2007CB507404)
基金Supported by the National Natural Science Foundation of China(21205005,81471919)MOST China(2011YQ0900502)1000 Plan
文摘Aging refers to a multidimensional process that all changes were accumulated in a person over time.These aging changes are associated with progressive increases in the chance of disease and death. Thus,it is necessary to establish a model to screen biomarkers to characterize and evaluate aging degree. In this study,an in vitro aging model was set up by formaldehdye and human serum albumin( HSA),the most abundant protein in human plasma,based on Maillard Reaction. The liquid chromatography tandem mass spectrometry( LC-MS /MS) method with ^(18)O-labeling technique was employed to quantify modification degree of peptides cleaved from HSA. This model was established and optimized for further quantitative biomarker study.
基金Supported by National Natural Science Foundation of China(21205005,81471919,21475010)MOST China(2011YQ0900502)+1 种基金1000 PlanResearch Foundation of China CDC(2014A101)
文摘The CP4-EPSPS gene is widely used in herbicide-tolerant plants/crops all over the world.In this study,a method was developed by coupling liquid chromatography with high sensitivity to tandem mass spectrometry to quantify the amount of CP4-EPSPS expression in Nicotiana tabacum leaves.The quantification of protein was converted to measure the unique peptide of CP4-EPSPS protein.One peptide unique to CP4-EPSPS was synthesized and labeled with H18 8 2O to get 1O stable isotope labeled peptide.The peptide served as the internal standard.The validated method had good specificity and linearity.The intra-and inter-day precisions and accuracy for all samples were satisfactory.The results demonstrated that the novel method was sensitive and selective to quantify CP4-EPSPS in the crude extract without time-consuming pre-separation or the purification procedures.
