[Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying bio...[Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying biological mechanism of embryo implantation and uterine diseases. [Method] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique were used to isolate BESC and BEGEC, then immunocytochemical method and TRYPAN-Blue assay were used to determine the purity and survival rate of isolated cells. [Result] The BESC and BEGEC were successfully isolated and cultured while immunocytochemical method and cell count method demonstrated that the purity was over 90%. The result of TRYPAN-Blue assay shown that survival rate of BESC and BEGEC was 91% and 78% respectively. [Conclusion] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique could isolate BESC and BEGEC with high purity.展开更多
为优化江川区土地利用空间格局,促进土地可持续发展,该文以云南省玉溪市江川区为研究区,将CLUE-S(conversion of land use and its effects at small region extent)模型和生态敏感性分析应用于江川区土地利用空间格局优化配置。以高度...为优化江川区土地利用空间格局,促进土地可持续发展,该文以云南省玉溪市江川区为研究区,将CLUE-S(conversion of land use and its effects at small region extent)模型和生态敏感性分析应用于江川区土地利用空间格局优化配置。以高度敏感区为CLUE-S模型中区域约束文件,突出研究区生态保护重点区域,并以综合生态敏感性分区为影响研究区土地利用变化的驱动力因子,提出一种将生态敏感性融入土地利用空间格局优化配置的过程和结果中的土地利用情景模拟方法,并借助2006年和2016年土地利用数据对江川区自然发展情景和土地生态优化情景2种模式下2025年的土地利用空间格局进行优化配置,并基于生态敏感性分区对优化配置模拟结果进行对比分析。结果表明:2种情景下2025年江川区土地利用格局既有共同特征也存在差异。在2种情景下均有效的保护了原有林地、耕地、未利用地和建设用地格局,但新增林地、草地等空间分布格局不同。根据2025年2种情景与2016年土地利用现状在不同敏感性区内主要土地利用类型分布情况表明,情景二土地利用空间格局优于自然发展情景和2016年土地利用现状。研究结果认为土地生态优化情景更为合理,该结果可为江川区生态保护和城镇发展提供参考。展开更多
基金Supported by the Innovation Foundation For Postgraduate of Guangxi University(2008105930905D001) the Tackle Key Program in Science and Technology of Science and Technology Bureau of Guangxi Province(0815008-2-4)~~
文摘[Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying biological mechanism of embryo implantation and uterine diseases. [Method] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique were used to isolate BESC and BEGEC, then immunocytochemical method and TRYPAN-Blue assay were used to determine the purity and survival rate of isolated cells. [Result] The BESC and BEGEC were successfully isolated and cultured while immunocytochemical method and cell count method demonstrated that the purity was over 90%. The result of TRYPAN-Blue assay shown that survival rate of BESC and BEGEC was 91% and 78% respectively. [Conclusion] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique could isolate BESC and BEGEC with high purity.
文摘为优化江川区土地利用空间格局,促进土地可持续发展,该文以云南省玉溪市江川区为研究区,将CLUE-S(conversion of land use and its effects at small region extent)模型和生态敏感性分析应用于江川区土地利用空间格局优化配置。以高度敏感区为CLUE-S模型中区域约束文件,突出研究区生态保护重点区域,并以综合生态敏感性分区为影响研究区土地利用变化的驱动力因子,提出一种将生态敏感性融入土地利用空间格局优化配置的过程和结果中的土地利用情景模拟方法,并借助2006年和2016年土地利用数据对江川区自然发展情景和土地生态优化情景2种模式下2025年的土地利用空间格局进行优化配置,并基于生态敏感性分区对优化配置模拟结果进行对比分析。结果表明:2种情景下2025年江川区土地利用格局既有共同特征也存在差异。在2种情景下均有效的保护了原有林地、耕地、未利用地和建设用地格局,但新增林地、草地等空间分布格局不同。根据2025年2种情景与2016年土地利用现状在不同敏感性区内主要土地利用类型分布情况表明,情景二土地利用空间格局优于自然发展情景和2016年土地利用现状。研究结果认为土地生态优化情景更为合理,该结果可为江川区生态保护和城镇发展提供参考。
