采用PCR-DGGE技术分析狼、羊长角血蜱体内携带菌群情况。无菌条件下将样品用液氮研磨成粉,试剂盒提取细菌总DNA;通用引物扩增细菌16S r DNA V3区并进行DGGE电泳,分析、选取7条优势条带,回收克隆测序。结果显示,狼、羊长角血蜱体内共携带...采用PCR-DGGE技术分析狼、羊长角血蜱体内携带菌群情况。无菌条件下将样品用液氮研磨成粉,试剂盒提取细菌总DNA;通用引物扩增细菌16S r DNA V3区并进行DGGE电泳,分析、选取7条优势条带,回收克隆测序。结果显示,狼、羊长角血蜱体内共携带7种优势菌,其中兰斯格军团杆菌(Legionella lansingensis)、皮可克立克次氏体(Rickettsia peacockii)、RSV70AB1-2细菌(Bacterium SV70AB1-2)、JNCZJb F1细菌(Bacterium JNCZJb F1)为二者共有;不动杆菌属(Acinetobacter sp)和一种不可培养的细菌存在于狼源长角血蜱体内;拉乌尔特立克次氏体(Rickettsia raoultii)存在羊源长角血蜱体内。长角血蜱寄生在不同宿主体内所携带优势菌群存在一定差异。展开更多
Tributyl citrate （TBC） plasticizer has been selected to prepare the novel plasticized PC under different time and temperature. The TBC plasticization effect on PC T, mechanical properties and morphology has been inv...Tributyl citrate （TBC） plasticizer has been selected to prepare the novel plasticized PC under different time and temperature. The TBC plasticization effect on PC T, mechanical properties and morphology has been investigated by DMTA, DSC, tensile test and SEM. The results show that the TBC content in PC is controlled by plasticization time and temperature. The mass-loss test has conftnned a less lost rate of TBC in PC. The T declines gradually with increasing TBC content. The tensile modulus and strength of the plasticized PC also decrease with the increase of TBC content, and an approximate linear relationship is found to exist between the TBC content and the tensile modulus and strength. The SEM images show that significant changes have taken place on the surface and in the cross-section of plasticized thin PC sheet.展开更多
基金Funded by the Natural Science Foundation of Hubei Province(No.2010CDB04604)
文摘Tributyl citrate （TBC） plasticizer has been selected to prepare the novel plasticized PC under different time and temperature. The TBC plasticization effect on PC T, mechanical properties and morphology has been investigated by DMTA, DSC, tensile test and SEM. The results show that the TBC content in PC is controlled by plasticization time and temperature. The mass-loss test has conftnned a less lost rate of TBC in PC. The T declines gradually with increasing TBC content. The tensile modulus and strength of the plasticized PC also decrease with the increase of TBC content, and an approximate linear relationship is found to exist between the TBC content and the tensile modulus and strength. The SEM images show that significant changes have taken place on the surface and in the cross-section of plasticized thin PC sheet.
