In order to enhance the efficiency and specificity of anticancer drug delivery and realize intelligently controlled release,a new multi-functional nanoparticle drug carrier was synthesized.The drug carrier was prepare...In order to enhance the efficiency and specificity of anticancer drug delivery and realize intelligently controlled release,a new multi-functional nanoparticle drug carrier was synthesized.The drug carrier was prepared by functionalizing multi-walled carbon nanotubes(MWCNTs) with polyethylenimines(PEI),fluorescein isothiocyanate(FITC) and glycyrrhizic acid(GL).After detailed characterization,doxorubicin(DOX) was loaded onto the obtained MWCNT composites through π-π stacking interactions.The drug loading capacity of the GL-functionalized material was up to 92%,and the release behavior was significantly pH-sensitive.Release at pH = 5.8(typical of the tumor cell microenvironment) was much more rapid and reached a greater extent than release under normal physiological conditions(pH = 7.4).The modified MWCNTs had high biocompatibility with the liver cancer cell line SMMC-7721,but were able to induce cell death after 24 h incubation if loaded with DOX.Tests with shorter incubation time(2 h) were undertaken to investigate the selectivity of the MWCNT composites,showed that the nanocomposites could specifically target cancer cells.The above results suggest that the functionalized carbon nanotubes-based material has potential applications for targeted delivery and controlled release of anticancer drug.展开更多
To obtain the wound dressings which can accelerate healing effectively,vitamin E D-α-Tocopherol polyethylene glycol succinate(vitamin E TPGS),one of the common derivatives of the unstable vitamins E,was successfully ...To obtain the wound dressings which can accelerate healing effectively,vitamin E D-α-Tocopherol polyethylene glycol succinate(vitamin E TPGS),one of the common derivatives of the unstable vitamins E,was successfully incorporated into P(LLA-CL)nanofibers by electrospinning.Electron microscopy showed that the smooth cylindrical fibers were obtained,albeit with a small amount of beading visible for the vitamins-loaded fibers.The diameters of the P(LLA-CL)fibers decreased with the addition of vitamins.The incorporation of the vitamin E TPGS in the electrospun fibers was confirmed by Fourier transform infrared spectroscopy(FTIR).Moreover,X-ray diffraction(XRD)indicated that vitamin E TPGS existed in the amorphous physical form after electrospinning.Fibers containing vitamin E TPGS showed a sustained release profile over more than 100 h in vitro.Antibacterial tests demonstrated that fibers loaded with vitamin E TPGS were effective in inhibiting the growth of E.coli and S.aureus.MTT assay showed that the fibers could promote the proliferation of L929 fibroblasts.These results above demonstrate the potential of P(LLA-CL)/vitamins E TPGS(P/E)as advanced wound dressing materials.展开更多
A novel nanofiber composite poly(N-isopropylacrylamide)(PNIPAAm)/polyvinyl pyrrolidone(PVP)was successfully prepared by electrospinning.Analogous medicated fibers loaded with ketoprofen(KET)as a model drug were prepar...A novel nanofiber composite poly(N-isopropylacrylamide)(PNIPAAm)/polyvinyl pyrrolidone(PVP)was successfully prepared by electrospinning.Analogous medicated fibers loaded with ketoprofen(KET)as a model drug were prepared.X-ray diffraction(XRD)demonstrated that the drug was presented in the fibers with an amorphous form.Both scanning and transmission electron microscopy showed that the fibers had an even diameter and smooth surface,and no phase separation was observed.The KET loaded nanofibers did not affect the morphology of the fibers,and no drug aggregation was separated from the polymer fibers.Water contact angle measurements proved that the PNIPAAm/PVP fibers switched from hydrophilic to hydrophobic when the temperature increased the lower critical solution temperature of 32℃.In vitro drug release studies were also undertaken and the result indicated that the PNIPAAm/PVP blend nanofiber presented the properties of the two polymers,having temperature-sensitive systems with sustained release properties.In addition,MTT assay demonstrated that the nanofiber film was non-toxic and suitable for cell growth.Thus,the nanofiber can be used as thermoresponsive carriers for sustained release of poor water soluble drugs.展开更多
基金Science and Technology Commission of Shanghai Municipality,China(No.16410723700)"111 Project" Biomedical Textile Materials Science and Technology,China(No.B07024)the UK-China Joint Laboratory for Therapeutic Textiles
文摘In order to enhance the efficiency and specificity of anticancer drug delivery and realize intelligently controlled release,a new multi-functional nanoparticle drug carrier was synthesized.The drug carrier was prepared by functionalizing multi-walled carbon nanotubes(MWCNTs) with polyethylenimines(PEI),fluorescein isothiocyanate(FITC) and glycyrrhizic acid(GL).After detailed characterization,doxorubicin(DOX) was loaded onto the obtained MWCNT composites through π-π stacking interactions.The drug loading capacity of the GL-functionalized material was up to 92%,and the release behavior was significantly pH-sensitive.Release at pH = 5.8(typical of the tumor cell microenvironment) was much more rapid and reached a greater extent than release under normal physiological conditions(pH = 7.4).The modified MWCNTs had high biocompatibility with the liver cancer cell line SMMC-7721,but were able to induce cell death after 24 h incubation if loaded with DOX.Tests with shorter incubation time(2 h) were undertaken to investigate the selectivity of the MWCNT composites,showed that the nanocomposites could specifically target cancer cells.The above results suggest that the functionalized carbon nanotubes-based material has potential applications for targeted delivery and controlled release of anticancer drug.
基金Science and Technology Commission of Shanghai Municipality,China(No.16410723700)“111 Project”Biomedical Textile Materials Science and Technology,China(No.B07024)UK-China Joint Laboratory for Therapeutic Textiles Based at Donghua University
文摘To obtain the wound dressings which can accelerate healing effectively,vitamin E D-α-Tocopherol polyethylene glycol succinate(vitamin E TPGS),one of the common derivatives of the unstable vitamins E,was successfully incorporated into P(LLA-CL)nanofibers by electrospinning.Electron microscopy showed that the smooth cylindrical fibers were obtained,albeit with a small amount of beading visible for the vitamins-loaded fibers.The diameters of the P(LLA-CL)fibers decreased with the addition of vitamins.The incorporation of the vitamin E TPGS in the electrospun fibers was confirmed by Fourier transform infrared spectroscopy(FTIR).Moreover,X-ray diffraction(XRD)indicated that vitamin E TPGS existed in the amorphous physical form after electrospinning.Fibers containing vitamin E TPGS showed a sustained release profile over more than 100 h in vitro.Antibacterial tests demonstrated that fibers loaded with vitamin E TPGS were effective in inhibiting the growth of E.coli and S.aureus.MTT assay showed that the fibers could promote the proliferation of L929 fibroblasts.These results above demonstrate the potential of P(LLA-CL)/vitamins E TPGS(P/E)as advanced wound dressing materials.
基金Science and Technology Commission of Shanghai Municipality,China(No.16410723700)"111 Project"Biomedical Textile Materials Science and Technology,China(No.B07024)UK-China Joint Laboratory for Therapeutic Textiles Based at Donghua University
文摘A novel nanofiber composite poly(N-isopropylacrylamide)(PNIPAAm)/polyvinyl pyrrolidone(PVP)was successfully prepared by electrospinning.Analogous medicated fibers loaded with ketoprofen(KET)as a model drug were prepared.X-ray diffraction(XRD)demonstrated that the drug was presented in the fibers with an amorphous form.Both scanning and transmission electron microscopy showed that the fibers had an even diameter and smooth surface,and no phase separation was observed.The KET loaded nanofibers did not affect the morphology of the fibers,and no drug aggregation was separated from the polymer fibers.Water contact angle measurements proved that the PNIPAAm/PVP fibers switched from hydrophilic to hydrophobic when the temperature increased the lower critical solution temperature of 32℃.In vitro drug release studies were also undertaken and the result indicated that the PNIPAAm/PVP blend nanofiber presented the properties of the two polymers,having temperature-sensitive systems with sustained release properties.In addition,MTT assay demonstrated that the nanofiber film was non-toxic and suitable for cell growth.Thus,the nanofiber can be used as thermoresponsive carriers for sustained release of poor water soluble drugs.
