The morphological changes of the tight junction of the alveolar epithelium andthe capillary endothelium of the lungs after blast injury in rats were studied with freeze-etching elec-tron microscopic technique.The resu...The morphological changes of the tight junction of the alveolar epithelium andthe capillary endothelium of the lungs after blast injury in rats were studied with freeze-etching elec-tron microscopic technique.The results were as follows:1.The number of the discontiuities,discontinuities per μm,irregularity,and展开更多
A lot of research work has been carriod out on thc lungs after blast injury, but nomorphomctric study of the blast-injured lungs has ever been reported so far.The authors quantifiedthe morphological changes of the lun...A lot of research work has been carriod out on thc lungs after blast injury, but nomorphomctric study of the blast-injured lungs has ever been reported so far.The authors quantifiedthe morphological changes of the lungs with blast injury in rats with stereological methods.t wasfound that following the blast injury,the pulmonary capillary volume and the intcmal surface arca de-creased significantly,while the arithmetic and harmonic mean thickness of the air-blood barrier andthe volume of polymorphonuclear leucocytes and platelets in the capillaries incrcased markedly.Themorphometric oxygen diffusion capacity of the lungs was remarkably reduced mainly due to the de-crease of the conductance of the air-blood barrier and erythrocytcs.In addition,the changes of theoxygen diffussion capacity of the lungs were closely related to the partial pressure of arteria/blood oxy-gen.These facts imply that the damage to the pulmonary capillaries was one of the main causes ofalveolar bleeding and early respiratory insufficiency.It is possible that a mathematic relationshipcould be established between the intensity of the blast impact and the consequent morphologicalchanges of the injured lungs.展开更多
The morphological and functional changes of the lungs in the early phase of blast injurywere analyzed and related quantitatively in rats with microcomputer-assisted morphometry.It wasfound that（1）significant or part...The morphological and functional changes of the lungs in the early phase of blast injurywere analyzed and related quantitatively in rats with microcomputer-assisted morphometry.It wasfound that（1）significant or partial correlation exists between stereological and functional indices,（2）changes of the endothelium and epithelium rather than the interstitium contribute to the increase ofharmonic and arithmetic thickness(T<sub>ht</sub>,T<sub>at</sub>)of the air-blood barrier,and（3）the regression equa-tions are as follows:（a）PaO<sub>2</sub>=3.56+1.62DL（O<sub>2</sub>）,r=0.678,P=0.032:（b）PaO<sub>2</sub>=2.99+3.49DL（O<sub>2</sub>）-0.0005S<sub>A</sub>+0.003S<sub>c</sub>-38.2V<sub>c</sub>-0.257T<sub>ap</sub>-0.028Dt（O<sub>2</sub>）,r<sub>y</sub>=0.9999,P=0.008;（c）PaCO<sub>2</sub>=7.69+0.0001S<sub>A</sub>-4.35T<sub>at</sub>+19.7T<sub>ht</sub>-46.2T<sub>hp</sub>-4.4T<sub>aen</sub>-5.87T<sub>aep</sub>,r<sub>y</sub>=0.9999,P=0.014;（d）pH=3.068+0.0005S<sub>A</sub>-1.79T<sub>at</sub>+12.2T<sub>ht</sub>-50.8T<sub>hp</sub>-2.52T<sub>aint</sub>+0.69DL（O<sub>2</sub>）,r<sub>y</sub>=0.9999,P=0.10.The significance and application of stereology in theestablishment of the quantitative relationship between morphological and functional indices are dis-cussed.Mathematical models to relate morphometric parameters to functional changes are possible.展开更多
文摘The morphological changes of the tight junction of the alveolar epithelium andthe capillary endothelium of the lungs after blast injury in rats were studied with freeze-etching elec-tron microscopic technique.The results were as follows:1.The number of the discontiuities,discontinuities per μm,irregularity,and
文摘A lot of research work has been carriod out on thc lungs after blast injury, but nomorphomctric study of the blast-injured lungs has ever been reported so far.The authors quantifiedthe morphological changes of the lungs with blast injury in rats with stereological methods.t wasfound that following the blast injury,the pulmonary capillary volume and the intcmal surface arca de-creased significantly,while the arithmetic and harmonic mean thickness of the air-blood barrier andthe volume of polymorphonuclear leucocytes and platelets in the capillaries incrcased markedly.Themorphometric oxygen diffusion capacity of the lungs was remarkably reduced mainly due to the de-crease of the conductance of the air-blood barrier and erythrocytcs.In addition,the changes of theoxygen diffussion capacity of the lungs were closely related to the partial pressure of arteria/blood oxy-gen.These facts imply that the damage to the pulmonary capillaries was one of the main causes ofalveolar bleeding and early respiratory insufficiency.It is possible that a mathematic relationshipcould be established between the intensity of the blast impact and the consequent morphologicalchanges of the injured lungs.
基金This study was supported by the National Natural Sciences Foundation of China
文摘The morphological and functional changes of the lungs in the early phase of blast injurywere analyzed and related quantitatively in rats with microcomputer-assisted morphometry.It wasfound that（1）significant or partial correlation exists between stereological and functional indices,（2）changes of the endothelium and epithelium rather than the interstitium contribute to the increase ofharmonic and arithmetic thickness(T<sub>ht</sub>,T<sub>at</sub>)of the air-blood barrier,and（3）the regression equa-tions are as follows:（a）PaO<sub>2</sub>=3.56+1.62DL（O<sub>2</sub>）,r=0.678,P=0.032:（b）PaO<sub>2</sub>=2.99+3.49DL（O<sub>2</sub>）-0.0005S<sub>A</sub>+0.003S<sub>c</sub>-38.2V<sub>c</sub>-0.257T<sub>ap</sub>-0.028Dt（O<sub>2</sub>）,r<sub>y</sub>=0.9999,P=0.008;（c）PaCO<sub>2</sub>=7.69+0.0001S<sub>A</sub>-4.35T<sub>at</sub>+19.7T<sub>ht</sub>-46.2T<sub>hp</sub>-4.4T<sub>aen</sub>-5.87T<sub>aep</sub>,r<sub>y</sub>=0.9999,P=0.014;（d）pH=3.068+0.0005S<sub>A</sub>-1.79T<sub>at</sub>+12.2T<sub>ht</sub>-50.8T<sub>hp</sub>-2.52T<sub>aint</sub>+0.69DL（O<sub>2</sub>）,r<sub>y</sub>=0.9999,P=0.10.The significance and application of stereology in theestablishment of the quantitative relationship between morphological and functional indices are dis-cussed.Mathematical models to relate morphometric parameters to functional changes are possible.
