The large yellow croaker iridovirus(LYCIV)ATPase gene was cloned via PCR technique.Sequencing result of PCR product showed that it consisted of 720bp nuceleotides,encoding a protein of 240 amino acids in which a typic...The large yellow croaker iridovirus(LYCIV)ATPase gene was cloned via PCR technique.Sequencing result of PCR product showed that it consisted of 720bp nuceleotides,encoding a protein of 240 amino acids in which a typical ATP/GTP binding site motif was contained.Then the LYCIV ATPase gene was highly expressed in Hi5 insect cells using Baculovirus Bac To Bac expressioin system.SDS PAGE analysis indicated the expression product was about 31kD,and accounted for about 12% of the total cellular protein.Recombinant ATPase was purified by Ni NTA column.A good foundation had been laid down for studying of the functions of LYCIV ATPase.展开更多
文摘The large yellow croaker iridovirus(LYCIV)ATPase gene was cloned via PCR technique.Sequencing result of PCR product showed that it consisted of 720bp nuceleotides,encoding a protein of 240 amino acids in which a typical ATP/GTP binding site motif was contained.Then the LYCIV ATPase gene was highly expressed in Hi5 insect cells using Baculovirus Bac To Bac expressioin system.SDS PAGE analysis indicated the expression product was about 31kD,and accounted for about 12% of the total cellular protein.Recombinant ATPase was purified by Ni NTA column.A good foundation had been laid down for studying of the functions of LYCIV ATPase.
