[Objective] To carry out preliminary study on callus induction and prolifer- ation of Cylocarya pafiurus. [Method] The leaf and stem segments of Cylocarya paliurus as explants were cultured on MS, WPM and improved DKW...[Objective] To carry out preliminary study on callus induction and prolifer- ation of Cylocarya pafiurus. [Method] The leaf and stem segments of Cylocarya paliurus as explants were cultured on MS, WPM and improved DKW3 mediums, added 6-BA and IBA with different concentration ratios to explore the optimum medium for callus induction and proliferation. [Result] It was found that MS ＋ 4.0 mg/L 6-BA ＋ 2.0 mg/L IBA was the optimum medium for inducing stem callus of Cylocarya pali- urus, MS ＋ 2.0 mg/L 6-BA ＋ 0.5 mg/L IBA was the optimum medium for inducing leaf explants callus of Cylocarya paliurus, and MS ＋ 1.0 mg/L 6-BA ＋ 1.0 mg/L IBA was the optimum medium for callus proliferation. 200 mg/L Vitamin C was best to inhibit Cylocarya paliurus callus from browning with the browning rate of only 5.71%. [Conclusion] This study provided some theoretical basis for the establishment of tis- sue culture and rapid propagation system and the development of molecular breed- ing study of Cylocarya paliurus.展开更多
基金Supported by the Research and Development Project of National Forestry Public Welfare Industry(200904046)the Outstanding Scientific and Technological Innovation Team Project of Jiangsu Universities(Jiangsu Education Bureau[2009]No.10)the"Six Talents Peak"Project of Jiangsu Province~~
文摘[Objective] To carry out preliminary study on callus induction and prolifer- ation of Cylocarya pafiurus. [Method] The leaf and stem segments of Cylocarya paliurus as explants were cultured on MS, WPM and improved DKW3 mediums, added 6-BA and IBA with different concentration ratios to explore the optimum medium for callus induction and proliferation. [Result] It was found that MS ＋ 4.0 mg/L 6-BA ＋ 2.0 mg/L IBA was the optimum medium for inducing stem callus of Cylocarya pali- urus, MS ＋ 2.0 mg/L 6-BA ＋ 0.5 mg/L IBA was the optimum medium for inducing leaf explants callus of Cylocarya paliurus, and MS ＋ 1.0 mg/L 6-BA ＋ 1.0 mg/L IBA was the optimum medium for callus proliferation. 200 mg/L Vitamin C was best to inhibit Cylocarya paliurus callus from browning with the browning rate of only 5.71%. [Conclusion] This study provided some theoretical basis for the establishment of tis- sue culture and rapid propagation system and the development of molecular breed- ing study of Cylocarya paliurus.
