Embryogenic cell suspension cultures were obtained from calli developed from mature rice seeds of a Japonica type Italian cultivar, Roncarolo. Protoplasts were isolated and transformed by PEG treated method with plasm...Embryogenic cell suspension cultures were obtained from calli developed from mature rice seeds of a Japonica type Italian cultivar, Roncarolo. Protoplasts were isolated and transformed by PEG treated method with plasmid pHP23 carrying the NPTⅡ gene which encodes resistance to antibiotic G 418. Protoplast derived colonies were selected in presence of the inhibitor. Plants were regenerated and transplanted into soil in the green house. The presence of foreign gene in the regenerated plants was verified by PCR and Southern analysis.展开更多
文摘Embryogenic cell suspension cultures were obtained from calli developed from mature rice seeds of a Japonica type Italian cultivar, Roncarolo. Protoplasts were isolated and transformed by PEG treated method with plasmid pHP23 carrying the NPTⅡ gene which encodes resistance to antibiotic G 418. Protoplast derived colonies were selected in presence of the inhibitor. Plants were regenerated and transplanted into soil in the green house. The presence of foreign gene in the regenerated plants was verified by PCR and Southern analysis.
