A simple method for the preparation of Pd nanoparticles is described. The Pd nanoparticles were electrodeposited on the gold electrode surface from 0.5 mol/L H 2SO 4 solution containing 0.01 mol/L K 2PdCl 4 by applyin...A simple method for the preparation of Pd nanoparticles is described. The Pd nanoparticles were electrodeposited on the gold electrode surface from 0.5 mol/L H 2SO 4 solution containing 0.01 mol/L K 2PdCl 4 by applying potential step from 1.1 V to 0 V through 5 s. Scanning electron microscopy proved that the as-prepared Pd nanoparticles are uniformly distributed on the bare gold electrode surface. The resulted Pd nanoparticles film modified gold electrode possesses a high catalytic activity for the reduction of dissolved oxygen in 0.1 mol/L KCl solution.展开更多
文摘A simple method for the preparation of Pd nanoparticles is described. The Pd nanoparticles were electrodeposited on the gold electrode surface from 0.5 mol/L H 2SO 4 solution containing 0.01 mol/L K 2PdCl 4 by applying potential step from 1.1 V to 0 V through 5 s. Scanning electron microscopy proved that the as-prepared Pd nanoparticles are uniformly distributed on the bare gold electrode surface. The resulted Pd nanoparticles film modified gold electrode possesses a high catalytic activity for the reduction of dissolved oxygen in 0.1 mol/L KCl solution.
文摘目的观察尿毒症apo E-/-小鼠主动脉根部动脉粥样硬化病变,慢性肾功能不全患者血清和尿毒症毒素硫酸吲哚酚对巨噬细胞胆固醇转运受体的表达及胞内脂质聚集的影响。方法外科手术法建立尿毒症apo E-/-小鼠的动物模型,分别收集尿毒症apo E-/-小鼠、假手术apo E-/-小鼠、C57BL/6J小鼠的主动脉根部,行冰冻切片油红O染色,计算动脉粥样硬化斑块相对面积。收集慢性肾功能不全患者及肾功正常者血清,以不同浓度的慢性肾功能不全患者血清或不同浓度的硫酸吲哚酚干预巨噬细胞株12 h,测定不同干预条件下胆固醇转运受体SR-A1、CD36、ABCA1、ABCG1、SR-B1 m RNA的表达;干预24 h后诱导泡沫细胞,油红O染色测定不同干预条件下细胞内脂质含量。结果尿毒症apo E-/-小鼠主动脉根部动脉粥样硬化斑块面积较假手术apo E-/-小鼠明显增大。5%的慢性肾功能不全患者血清及250μmol/L的硫酸吲哚酚可明显诱导巨噬细胞CD36 m RNA的表达而不影响其余胆固醇转运受体的表达,同时增加巨噬细胞内脂质的蓄积。结论慢性肾功能不全加速动脉粥样硬化的进展,机制与慢性肾功能不全血清中蛋白结合性尿毒症毒素硫酸吲哚酚诱导巨噬细胞CD36 m RNA表达上调、促进细胞内的脂质蓄积有助于巨噬细胞源性泡沫细胞形成有关。
