在教育部高等学校课程思政建设要求下,结合环境工程专业人才培养目标和课程特点,开展环境工程微生物学实验马克思主义理论课程思政,探索思政建设融合模式和实施路径。通过将马克思主义理论融入教学内容从而发挥育人作用、以及用马克思...在教育部高等学校课程思政建设要求下,结合环境工程专业人才培养目标和课程特点,开展环境工程微生物学实验马克思主义理论课程思政,探索思政建设融合模式和实施路径。通过将马克思主义理论融入教学内容从而发挥育人作用、以及用马克思主义观点方法指导学生学习方法学习态度,使专业教学与思想教育深度融合。具体的模式是:将环境工程微生物学实验的教学内容分成几个模块,不同的模块可以分别融入马克思主义科学自然观、马克思主义科学实践观和马克思主义科学幸福观等思政元素;同时运用马克思主义的观点方法来指导学生观察问题和分析问题,培养学生严谨的学习态度和解决问题的能力。环境工程微生物学实验马克思主义理论课程思政的实施,提高了学生的思想水平、专业学习效率和实践能力,有利于高校高素质人才的培养。Under the requirements of ideological and political construction of higher education of the Ministry of Education, combined with the training objectives and curriculum characteristics of environmental engineering majors, the ideological and political courses of environmental engineering microbiology experiment are carried out, and the integration mode and implementation path of ideological and political construction are explored. By integrating Marxist theory into the teaching content so as to play the role of education, and guiding students’ learning methods with Marxist viewpoints and methods, professional teaching and ideological education are deeply integrated. The specific model is to divide the teaching content of environmental engineering microbiology experiments into several modules, each of which can be integrated with ideological and political elements such as the Marxist scientific view of nature, the Marxist scientific view of practice, and the Marxist scientific view of happiness. At the same time, the Marxist perspective and methodology are applied to guide students in observing and analyzing problems, cultivating their rigorous learning attitude and problem-solving skills. The implementation of ideological and political courses of Marxist theory has improved students’ ideological level, professional learning efficiency and practical ability, which is conducive to the cultivation of high-quality talents in colleges and universities.展开更多
为建立气相–质谱联用技术(GC-MS)分析柳杉叶挥发油成分,并研究柳杉叶挥发油的抗菌活性。本研究以柳杉叶为材料,采用水蒸汽蒸馏法提取柳杉叶挥发油;以三种常见细菌和一种植物病原真菌为作用对象,利用梯度稀释法对柳杉叶挥发油进行抗菌...为建立气相–质谱联用技术(GC-MS)分析柳杉叶挥发油成分,并研究柳杉叶挥发油的抗菌活性。本研究以柳杉叶为材料,采用水蒸汽蒸馏法提取柳杉叶挥发油;以三种常见细菌和一种植物病原真菌为作用对象,利用梯度稀释法对柳杉叶挥发油进行抗菌活性研究;利用GC-MS分析鉴定柳杉叶挥发油的主要化学成分,并且运用峰面积归一法确定各个成分的含量。研究结果表明:柳杉叶挥发油对产气肠杆菌、大肠杆菌、金黄色葡萄球菌和尖孢镰刀菌均有一定的抑制作用,其中对0.8 g∙mL−1尖孢镰刀菌的抗菌率为39.8%;柳杉叶挥发油的主要化学成分为萜类和醇类,含量较多的是(−)-扁枝烯(24.81%)、榄香醇(16.91%)、表桉叶油醇(8.72%)、α-蒎烯(7.06%)、β-芬兰烯(6.71%)、D-柠檬烯(4.81%)。因此,柳杉叶挥发油具有抗菌作用,萜类可能是主要有效抗菌成分。In order to establish a gas phase mass spectrometry (GC-MS) technique for the analysis of volatile oil components of Cryptomeria fortunei leaves and to study the antibacterial activity of the volatile oil. In this study, the volatile oil of Cryptomeria fortunei leaves was extracted by steam distillation, and the antibacterial activity of the volatile oil was studied by gradient dilution method with three common bacteria and one plant pathogen fungus as the target. The main chemical components of the volatile oil of Cryptomeria fortunei leaves were identified by GC-MS, and the content of each component was determined by peak area normalization method. The results showed that the volatile oil of Cryptomeria fortunei leaves had certain inhibitory effects on Enterobacter aerogenes, Escherichia coli, Staphylococcus aureus and Fusarium oxysporum, and the antibacterial rate against 0.8 g·mL−1 Fusarium oxysporum was 39.8%. The main components of the volatile oil of Cryptomeria fortunei leaves were terpenes and alcohols, the most abundant of which were (−)-platydene (24.81%), elemenol (16.91%), eutectol (8.72%), α-pinene (7.06%), β-Finlanene (6.71%) and D-limonene (4.81%). Therefore, the volatile oil of Cryptomeria fortunei leaves has antibacterial effect, and terpenoids may be the main effective antibacterial components.展开更多
文摘在教育部高等学校课程思政建设要求下,结合环境工程专业人才培养目标和课程特点,开展环境工程微生物学实验马克思主义理论课程思政,探索思政建设融合模式和实施路径。通过将马克思主义理论融入教学内容从而发挥育人作用、以及用马克思主义观点方法指导学生学习方法学习态度,使专业教学与思想教育深度融合。具体的模式是:将环境工程微生物学实验的教学内容分成几个模块,不同的模块可以分别融入马克思主义科学自然观、马克思主义科学实践观和马克思主义科学幸福观等思政元素;同时运用马克思主义的观点方法来指导学生观察问题和分析问题,培养学生严谨的学习态度和解决问题的能力。环境工程微生物学实验马克思主义理论课程思政的实施,提高了学生的思想水平、专业学习效率和实践能力,有利于高校高素质人才的培养。Under the requirements of ideological and political construction of higher education of the Ministry of Education, combined with the training objectives and curriculum characteristics of environmental engineering majors, the ideological and political courses of environmental engineering microbiology experiment are carried out, and the integration mode and implementation path of ideological and political construction are explored. By integrating Marxist theory into the teaching content so as to play the role of education, and guiding students’ learning methods with Marxist viewpoints and methods, professional teaching and ideological education are deeply integrated. The specific model is to divide the teaching content of environmental engineering microbiology experiments into several modules, each of which can be integrated with ideological and political elements such as the Marxist scientific view of nature, the Marxist scientific view of practice, and the Marxist scientific view of happiness. At the same time, the Marxist perspective and methodology are applied to guide students in observing and analyzing problems, cultivating their rigorous learning attitude and problem-solving skills. The implementation of ideological and political courses of Marxist theory has improved students’ ideological level, professional learning efficiency and practical ability, which is conducive to the cultivation of high-quality talents in colleges and universities.
文摘为建立气相–质谱联用技术(GC-MS)分析柳杉叶挥发油成分,并研究柳杉叶挥发油的抗菌活性。本研究以柳杉叶为材料,采用水蒸汽蒸馏法提取柳杉叶挥发油;以三种常见细菌和一种植物病原真菌为作用对象,利用梯度稀释法对柳杉叶挥发油进行抗菌活性研究;利用GC-MS分析鉴定柳杉叶挥发油的主要化学成分,并且运用峰面积归一法确定各个成分的含量。研究结果表明:柳杉叶挥发油对产气肠杆菌、大肠杆菌、金黄色葡萄球菌和尖孢镰刀菌均有一定的抑制作用,其中对0.8 g∙mL−1尖孢镰刀菌的抗菌率为39.8%;柳杉叶挥发油的主要化学成分为萜类和醇类,含量较多的是(−)-扁枝烯(24.81%)、榄香醇(16.91%)、表桉叶油醇(8.72%)、α-蒎烯(7.06%)、β-芬兰烯(6.71%)、D-柠檬烯(4.81%)。因此,柳杉叶挥发油具有抗菌作用,萜类可能是主要有效抗菌成分。In order to establish a gas phase mass spectrometry (GC-MS) technique for the analysis of volatile oil components of Cryptomeria fortunei leaves and to study the antibacterial activity of the volatile oil. In this study, the volatile oil of Cryptomeria fortunei leaves was extracted by steam distillation, and the antibacterial activity of the volatile oil was studied by gradient dilution method with three common bacteria and one plant pathogen fungus as the target. The main chemical components of the volatile oil of Cryptomeria fortunei leaves were identified by GC-MS, and the content of each component was determined by peak area normalization method. The results showed that the volatile oil of Cryptomeria fortunei leaves had certain inhibitory effects on Enterobacter aerogenes, Escherichia coli, Staphylococcus aureus and Fusarium oxysporum, and the antibacterial rate against 0.8 g·mL−1 Fusarium oxysporum was 39.8%. The main components of the volatile oil of Cryptomeria fortunei leaves were terpenes and alcohols, the most abundant of which were (−)-platydene (24.81%), elemenol (16.91%), eutectol (8.72%), α-pinene (7.06%), β-Finlanene (6.71%) and D-limonene (4.81%). Therefore, the volatile oil of Cryptomeria fortunei leaves has antibacterial effect, and terpenoids may be the main effective antibacterial components.
