Three anti-EGF receptor MoAbs were used in these studies. Administration of MoAbs 3 and 176 inhibited tumor formation in nude mice by CNE-2, a poorly differentiated nasopharyngeal carcinoma cell line and A431, an epid...Three anti-EGF receptor MoAbs were used in these studies. Administration of MoAbs 3 and 176 inhibited tumor formation in nude mice by CNE-2, a poorly differentiated nasopharyngeal carcinoma cell line and A431, an epidermoid carcinoma cell line. When the same MoAbs were used in treatment against HeLa, a cervical carcinoma, tumor growth was not affected. The number of EGF receptors and apparent dissociation constants for ^(125)I-EGF on CNE-2 and A431 was 1.3×10^(?)/cell (Kd 7.7×10^(-8)mol/L) and 1.4×10~6/cell(Kd 2.4×10^(-9)mol/L), respectively. Both MoAbs 3 and 176, capable of competing with EGF for receptor binding, showed significant tumor growth inhibition. MoAb 101 was incapable of blocking the binding of EGF to its receptor, and not as effective as MoAbs 3 and 176 in tumor growth inhibition. Our observation is that the MoAb anti-EGF receptor is cytostatic rather than cytocidal, in vitro against CNE-2 and A431.展开更多
Isolation and characterization of yeast mannan from Saccharomyces cerivisiae were studied. It was demonstrated that mannan was completely composed of single sugar D-mannose with molecular weight of 80KD and [α]D20+87...Isolation and characterization of yeast mannan from Saccharomyces cerivisiae were studied. It was demonstrated that mannan was completely composed of single sugar D-mannose with molecular weight of 80KD and [α]D20+87° （0.23%, water）, and contained 99.5% carbohydrate and 0.13% protein.The freshly prepared samples and old ones give sharp peaks and展开更多
文摘Three anti-EGF receptor MoAbs were used in these studies. Administration of MoAbs 3 and 176 inhibited tumor formation in nude mice by CNE-2, a poorly differentiated nasopharyngeal carcinoma cell line and A431, an epidermoid carcinoma cell line. When the same MoAbs were used in treatment against HeLa, a cervical carcinoma, tumor growth was not affected. The number of EGF receptors and apparent dissociation constants for ^(125)I-EGF on CNE-2 and A431 was 1.3×10^(?)/cell (Kd 7.7×10^(-8)mol/L) and 1.4×10~6/cell(Kd 2.4×10^(-9)mol/L), respectively. Both MoAbs 3 and 176, capable of competing with EGF for receptor binding, showed significant tumor growth inhibition. MoAb 101 was incapable of blocking the binding of EGF to its receptor, and not as effective as MoAbs 3 and 176 in tumor growth inhibition. Our observation is that the MoAb anti-EGF receptor is cytostatic rather than cytocidal, in vitro against CNE-2 and A431.
文摘Isolation and characterization of yeast mannan from Saccharomyces cerivisiae were studied. It was demonstrated that mannan was completely composed of single sugar D-mannose with molecular weight of 80KD and [α]D20+87° （0.23%, water）, and contained 99.5% carbohydrate and 0.13% protein.The freshly prepared samples and old ones give sharp peaks and
