The optimum medium and fermentations conditions of the lipase inhibitor producing strain Bacillus sp. LF were studied. The optimal medium was selected after single factor test and orthogonal test as follows: lactose 0...The optimum medium and fermentations conditions of the lipase inhibitor producing strain Bacillus sp. LF were studied. The optimal medium was selected after single factor test and orthogonal test as follows: lactose 0.8%, peptone 1%,（NH4）2SO4 0.8%,NaCl 0.5%,original pH 7.2. With 4%（ V/V ） inoculum in 40 mL medium in 250 mL flask, Bacillus sp. LF could reach high yield of lipase inhibitor after 24 h at 30℃. The highest lipase inhibitory activity in broth could reach 52.3%.展开更多
文摘The optimum medium and fermentations conditions of the lipase inhibitor producing strain Bacillus sp. LF were studied. The optimal medium was selected after single factor test and orthogonal test as follows: lactose 0.8%, peptone 1%,（NH4）2SO4 0.8%,NaCl 0.5%,original pH 7.2. With 4%（ V/V ） inoculum in 40 mL medium in 250 mL flask, Bacillus sp. LF could reach high yield of lipase inhibitor after 24 h at 30℃. The highest lipase inhibitory activity in broth could reach 52.3%.
