Spectrin-like protein has been found in a variety of plant cells. In this study, electron microscopic observation of immuno-gold labelled preparations from the leaf petiole of cucumber ( Cucumis sativus L.) shows that...Spectrin-like protein has been found in a variety of plant cells. In this study, electron microscopic observation of immuno-gold labelled preparations from the leaf petiole of cucumber ( Cucumis sativus L.) shows that it also exists in the sieve element-companion cell (SE-CC) complex, being widely distributed in P-protein filaments and sieve element reticulum (SER), in the cytoplasm and mitochondrial membrane of companion cell (CC) and in the branched plasmodesmata between sieve element (SE) and CC as well. The results suggest that this protein could be synthesized in CC and transferred to SE through plasmodesmata. Western blotting showed that spectrin-like protein existed in the protein of phloem exudate of cucumber, and its molecular weight was about 260 kD.展开更多
达尔文在观察西蕃莲(Passi flora)卷须快速弯曲运动时提出,卷须的快速运动可能有电波传递操纵,近些年陆续在卷须中观察到电波传递,并在卷领中分离出肌动球蛋白,在含羞草(Mimosa)上也观察到机械刺激以后叶片下垂运动和原生质收缩的关系....达尔文在观察西蕃莲(Passi flora)卷须快速弯曲运动时提出,卷须的快速运动可能有电波传递操纵,近些年陆续在卷须中观察到电波传递,并在卷领中分离出肌动球蛋白,在含羞草(Mimosa)上也观察到机械刺激以后叶片下垂运动和原生质收缩的关系.这些发现揭示:广泛存在于动物界和“神经-肌肉”兴奋传递机理也可能在某种程度上存在于植物界并执行传递兴奋,操纵运动的功能.近几年我们以丝瓜(Luffa of ficinale)卷须为材料,对这种机理进行了系统观察,本文把这些工作的进展简要报道如下.展开更多
文摘Spectrin-like protein has been found in a variety of plant cells. In this study, electron microscopic observation of immuno-gold labelled preparations from the leaf petiole of cucumber ( Cucumis sativus L.) shows that it also exists in the sieve element-companion cell (SE-CC) complex, being widely distributed in P-protein filaments and sieve element reticulum (SER), in the cytoplasm and mitochondrial membrane of companion cell (CC) and in the branched plasmodesmata between sieve element (SE) and CC as well. The results suggest that this protein could be synthesized in CC and transferred to SE through plasmodesmata. Western blotting showed that spectrin-like protein existed in the protein of phloem exudate of cucumber, and its molecular weight was about 260 kD.
文摘达尔文在观察西蕃莲(Passi flora)卷须快速弯曲运动时提出,卷须的快速运动可能有电波传递操纵,近些年陆续在卷须中观察到电波传递,并在卷领中分离出肌动球蛋白,在含羞草(Mimosa)上也观察到机械刺激以后叶片下垂运动和原生质收缩的关系.这些发现揭示:广泛存在于动物界和“神经-肌肉”兴奋传递机理也可能在某种程度上存在于植物界并执行传递兴奋,操纵运动的功能.近几年我们以丝瓜(Luffa of ficinale)卷须为材料,对这种机理进行了系统观察,本文把这些工作的进展简要报道如下.
