There are various strategies to conduct tumor microenvironment(TME)stimulus-responsive(e.g.,acid,H_(2)O_(2)or glutathione)nanoreactors for increasing the efficiency of chemodynamic therapy(CDT).Among these,the exploit...There are various strategies to conduct tumor microenvironment(TME)stimulus-responsive(e.g.,acid,H_(2)O_(2)or glutathione)nanoreactors for increasing the efficiency of chemodynamic therapy(CDT).Among these,the exploitation of adenosine triphosphate(ATP,another overexpressed biomarker in TME)-responsive nanoreactors for tumor CDT is still challenging.Herein,the ATP-responsive iron-doped CDs(FeCDs)were firstly prepared and then coassembled with glucose oxidase(GOx)to obtain FeCDs/GOx liposomes as ATP-responsive nanoreactors.Under TME conditions,the nanoreactors initially released FeCDs and GOx.Subsequently,with the existence of ATP,iron ions were rapidly released from the FeCDs to trigger Fenton/Fenton-like reactions for generating·OH.Meanwhile,the T_(1)-weighted magnetic resonance imaging(MRI)was achieved due to the released iron ions.Moreover,the GOx converted endogenous glucose in tumor to gluconic acid and H_(2)O_(2)to satisfy the requirement of·OH generation.In vitro as well as in vivo experiments illustrated that the obtained ATP-responsive CD nanoreactors could be used as a versatile nanotheranostics for simultaneously T_(1)-weighted MRI-guided tumor CDT.This work presents a new ATP-responsive nanoreactor with selfsupplied H_(2)O_(2)for multifunctional nanotheranostic applications.展开更多
This paper reports on new applications of water-dispersible graphene quantum dots(GQDs) that we recently developed. The prepared GQDs not only show broad absorption in the visible spectrum from 400 to 700 nm, but can ...This paper reports on new applications of water-dispersible graphene quantum dots(GQDs) that we recently developed. The prepared GQDs not only show broad absorption in the visible spectrum from 400 to 700 nm, but can also serve as smart photosensitizers with high singlet oxygen(1O2) production under visible-light irradiation(≥420 nm). We showed that the prepared GQDs can potentially be used as a metal-free, visible-light-active, sensitized photocatalyst via energy transfer mechanism, in which the light energy was converted by GQDs to produce 1O2, which can kill nearby microorganisms and degrade organic dyes.展开更多
The effect of tumor-targeted photodynamic therapy（PDT） was improved by designing nanotheranostics to promote oxygenation in a tumor microenvironment（TME）wherein hypoxia, acidosis, and the elevated levels of H2O2 a...The effect of tumor-targeted photodynamic therapy（PDT） was improved by designing nanotheranostics to promote oxygenation in a tumor microenvironment（TME）wherein hypoxia, acidosis, and the elevated levels of H2O2 are three main characteristics. In this study, a carbon dot（CD）PDT agent recently developed by our group was firstly applied as reducing agent to react with potassium permanganate for fabricating CDs/manganese dioxide（CDs/MnO2） composites,which were in turn modified with polyethylene glycol（PEG） to form water-soluble CDs/MnO2-PEG nanohybrids. In a normal physiological environment, the as-prepared nanohybrids exhibited quenched fluorescence, weak singlet oxygen generation, and low magnetic resonance imaging（MRI） signal.However, given the high sensitivity of MnO2 to the TME, the CDs/MnO2-PEG nanohybrids changed from an ＂off＂ to an＂on＂ state with synchronously enhanced fluorescence, singlet oxygen generation, and MRI signal in the TME. In vitro and in vivo analyses have revealed that CDs/MnO2-PEG nanohybrids could be applied as TME-driven, turn-on nanotheranostics for the MR/fluorescence bimodal imaging-guided PDT of cancer.Moreover, complete clearance of CDs/MnO2-PEG nanohybrids from the body of mice was observed, indicating their low long-term toxicity and good biocompatibility. This work offers a new nanotheranostic candidate for modulating the unfavorable TME, particularly for the targeted PDT of cancer through precise positioning and oxygen generation.展开更多
One-dimensional silicon nanorod（Si NR） has attracted considerable interest because of its unique morphology and electronic-optical properties that render Si NRs suitable for a broad spectrum of applications, such as...One-dimensional silicon nanorod（Si NR） has attracted considerable interest because of its unique morphology and electronic-optical properties that render Si NRs suitable for a broad spectrum of applications, such as fieldeffect transistor, drug carrier, solar cell, nanomechanical device, and lithium-ion battery. However, studies aiming to identify a new synthetic method and apply Si NR in the biomedical field remain limited. This study is the first to use an ethylene glycol-mediated synthetic route to prepare Si NR as a multicolor fluorescent probe and a new photodynamic therapy（PDT） agent. The as-prepared Si NR demonstrates bright fluorescence, excellent storage and photostability, favorable biocompatibility, excitation-dependent emission, and measurable quantity of-1O2（0.24）. On the basis of these features,we demonstrate through in vitro studies that the Si NR can be utilized as a new nanophotosensitizer for fluorescence imaging-guided cancer treatment. Our work leads to a new production process for Si NRs that can be used not only as PDT agents for therapy of shallow tissue cancer but also as excellent, environment-friendly, and red light-induced photocatalysts for the degradation of persistent organic pollutants in the future.展开更多
基金supported by the National Key Research and Development Program of China(2022YFA1207600)National Natural Science Foundation of China(51972315,21873110,52272052,61720106014)project ZR2023QE322 supported by Shandong Provincial Natural Science Foundation。
文摘There are various strategies to conduct tumor microenvironment(TME)stimulus-responsive(e.g.,acid,H_(2)O_(2)or glutathione)nanoreactors for increasing the efficiency of chemodynamic therapy(CDT).Among these,the exploitation of adenosine triphosphate(ATP,another overexpressed biomarker in TME)-responsive nanoreactors for tumor CDT is still challenging.Herein,the ATP-responsive iron-doped CDs(FeCDs)were firstly prepared and then coassembled with glucose oxidase(GOx)to obtain FeCDs/GOx liposomes as ATP-responsive nanoreactors.Under TME conditions,the nanoreactors initially released FeCDs and GOx.Subsequently,with the existence of ATP,iron ions were rapidly released from the FeCDs to trigger Fenton/Fenton-like reactions for generating·OH.Meanwhile,the T_(1)-weighted magnetic resonance imaging(MRI)was achieved due to the released iron ions.Moreover,the GOx converted endogenous glucose in tumor to gluconic acid and H_(2)O_(2)to satisfy the requirement of·OH generation.In vitro as well as in vivo experiments illustrated that the obtained ATP-responsive CD nanoreactors could be used as a versatile nanotheranostics for simultaneously T_(1)-weighted MRI-guided tumor CDT.This work presents a new ATP-responsive nanoreactor with selfsupplied H_(2)O_(2)for multifunctional nanotheranostic applications.
基金supported by the National Natural Science Foundation of China(6122700851472252+2 种基金51172244and 11179006)the Key Research Program of the Chinese Academy of Sciences(KGZD-EW-T02)
文摘This paper reports on new applications of water-dispersible graphene quantum dots(GQDs) that we recently developed. The prepared GQDs not only show broad absorption in the visible spectrum from 400 to 700 nm, but can also serve as smart photosensitizers with high singlet oxygen(1O2) production under visible-light irradiation(≥420 nm). We showed that the prepared GQDs can potentially be used as a metal-free, visible-light-active, sensitized photocatalyst via energy transfer mechanism, in which the light energy was converted by GQDs to produce 1O2, which can kill nearby microorganisms and degrade organic dyes.
基金supported by the National Natural Science Foundation of China (51472252 and 51572269)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB17000000)
文摘The effect of tumor-targeted photodynamic therapy（PDT） was improved by designing nanotheranostics to promote oxygenation in a tumor microenvironment（TME）wherein hypoxia, acidosis, and the elevated levels of H2O2 are three main characteristics. In this study, a carbon dot（CD）PDT agent recently developed by our group was firstly applied as reducing agent to react with potassium permanganate for fabricating CDs/manganese dioxide（CDs/MnO2） composites,which were in turn modified with polyethylene glycol（PEG） to form water-soluble CDs/MnO2-PEG nanohybrids. In a normal physiological environment, the as-prepared nanohybrids exhibited quenched fluorescence, weak singlet oxygen generation, and low magnetic resonance imaging（MRI） signal.However, given the high sensitivity of MnO2 to the TME, the CDs/MnO2-PEG nanohybrids changed from an ＂off＂ to an＂on＂ state with synchronously enhanced fluorescence, singlet oxygen generation, and MRI signal in the TME. In vitro and in vivo analyses have revealed that CDs/MnO2-PEG nanohybrids could be applied as TME-driven, turn-on nanotheranostics for the MR/fluorescence bimodal imaging-guided PDT of cancer.Moreover, complete clearance of CDs/MnO2-PEG nanohybrids from the body of mice was observed, indicating their low long-term toxicity and good biocompatibility. This work offers a new nanotheranostic candidate for modulating the unfavorable TME, particularly for the targeted PDT of cancer through precise positioning and oxygen generation.
基金supported by the National NaturalScience Foundation of China (51472252 and 51572269)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB17030400)
文摘One-dimensional silicon nanorod（Si NR） has attracted considerable interest because of its unique morphology and electronic-optical properties that render Si NRs suitable for a broad spectrum of applications, such as fieldeffect transistor, drug carrier, solar cell, nanomechanical device, and lithium-ion battery. However, studies aiming to identify a new synthetic method and apply Si NR in the biomedical field remain limited. This study is the first to use an ethylene glycol-mediated synthetic route to prepare Si NR as a multicolor fluorescent probe and a new photodynamic therapy（PDT） agent. The as-prepared Si NR demonstrates bright fluorescence, excellent storage and photostability, favorable biocompatibility, excitation-dependent emission, and measurable quantity of-1O2（0.24）. On the basis of these features,we demonstrate through in vitro studies that the Si NR can be utilized as a new nanophotosensitizer for fluorescence imaging-guided cancer treatment. Our work leads to a new production process for Si NRs that can be used not only as PDT agents for therapy of shallow tissue cancer but also as excellent, environment-friendly, and red light-induced photocatalysts for the degradation of persistent organic pollutants in the future.
