期刊文献+
共找到6篇文章
< 1 >
每页显示 20 50 100
空白及载阿霉素的聚丙烯酸栓塞微球的制备与评价 被引量:4
1
作者 郭李盈 刘晓昕 +7 位作者 李子圆 覃小雅 范则杨 李真真 关海涛 宋莉 邹英华 范田园 《北京大学学报(医学版)》 CAS CSCD 北大核心 2018年第6期1070-1077,共8页
目的:研究离子交换型载阿霉素聚丙烯酸栓塞微球的制备方法与性质评价。方法:采用反相悬浮聚合法制备空白聚丙烯酸栓塞微球[poly (acrylic acid) microspheres,PMs],通过离子交换原理将阿霉素载入该微球,制备载阿霉素的聚丙烯酸栓塞微球[... 目的:研究离子交换型载阿霉素聚丙烯酸栓塞微球的制备方法与性质评价。方法:采用反相悬浮聚合法制备空白聚丙烯酸栓塞微球[poly (acrylic acid) microspheres,PMs],通过离子交换原理将阿霉素载入该微球,制备载阿霉素的聚丙烯酸栓塞微球[doxorubicin-loaded poly (acrylic acid) microspheres,DPMs]。采用光学显微镜考察两种微球的形态和粒径分布,荧光显微镜和激光扫描共聚焦显微镜考察载药微球中阿霉素的分布,物性测定仪测定PMs和DPMs的弹性,HPLC法测定PMs的载药性质和DPMs的释药性质。以0. 1 mL的DPMs栓塞家兔肝动脉评价其在动物体内的栓塞效果。结果:制备的PMs和DPMs形态圆整、表面光滑,能够均匀分散,阿霉素主要分布在靠近微球表面的区域且分布均匀。PMs和DPMs的平均粒径分别为(283±136)μm和(248±149)μm,杨氏模量分别为(62. 63±1. 65) k Pa和(93. 94±1. 10) k Pa,空白和载药微球均具有良好的抗压缩能力。PMs在12 h时达到载药平衡,包封率大于99%,在浓度为5. 0 g/L和12. 5 g/L的阿霉素溶液中微球的载药量分别为(19. 78±0. 27)g/L和(49. 45±0. 37) g/L; DPMs在磷酸盐缓冲液(phosphate buffered saline,PBS)中缓慢释放阿霉素,载药量为(19. 78±0. 27) g/L和(49. 45±0. 37) g/L的微球24 h体外累积释放百分数分别为6. 82%±0. 02%和2. 83%±0. 10%。影像学结果显示,DPMs成功地栓塞了家兔的肝动脉。结论:聚丙烯酸微球具有良好的载阿霉素性能,DPMs是一种潜在的可用于动脉化疗栓塞的新型药物递送系统。 展开更多
关键词 微球 化学栓塞 治疗性 离子交换 阿霉素 缓释
下载PDF
柠檬酸修饰的超顺磁性氧化铁纳米粒的制备及表征 被引量:2
2
作者 王欢 覃小雅 +2 位作者 李子圆 郑卓肇 范田园 《北京大学学报(医学版)》 CAS CSCD 北大核心 2018年第2期340-346,共7页
目的:制备并表征柠檬酸(citric acid,CA)修饰的超顺磁性氧化铁纳米粒(superparamagnetic iron oxide nanoparticles,SPIONs)用于磁靶向、热疗和磁共振成像(magnetic resonance imaging,MRI)。方法:应用共沉淀法制备柠檬酸修饰的SPIONs(C... 目的:制备并表征柠檬酸(citric acid,CA)修饰的超顺磁性氧化铁纳米粒(superparamagnetic iron oxide nanoparticles,SPIONs)用于磁靶向、热疗和磁共振成像(magnetic resonance imaging,MRI)。方法:应用共沉淀法制备柠檬酸修饰的SPIONs(CA-SPIONs),并通过磁铁、透射电子显微镜、激光粒度测定仪、傅里叶转换红外(Fourier transform infrared,FT-IR)光谱仪、热重-差热同步分析仪、振动样品磁强计、X射线衍射仪分别对CA-SPIONs的磁响应性、形态、粒径、红外特征、柠檬酸的质量分数、磁学性质、X射线衍射图谱等进行表征;采用高频感应加热机对CA-SPIONs的发热性能进行考察;通过3.0 T的MRI扫描仪对CA-SPIONs的横向弛豫效能(r2)进行评价。结果:制备的CA-SPIONs在水中分散性良好,呈深黑色,具有良好的磁响应性,其形态圆整、大小均一,平均粒径在12 nm左右,水力学平均粒径为(72.35±4.47)nm,多分散系数为0.231±0.029。红外光谱结果证明CA-SPIONs表面成功修饰上了CA,修饰的CA所占的重量百分比为9.0%。CA-SPIONs具有良好的超顺磁性,其饱和磁化强度为63.58 emu/g;CA-SPIONs为反尖晶石型结构,经Debye-Scherrer半宽公式可计算出晶粒大小为12.4 nm;在9 A和45~50 k Hz的交变电磁场下,CA-SPIONs呈现出良好的发热性能,比吸收率(specific absorption rate,SAR)值为26W/g;在3.0 T的MRI扫描仪下,测得CA-SPIONs的r2为338(mmol/L)-1·s-1,表明CA-SPIONs具有良好的MRI负性对比增强作用。结论:CA-SPIONs有希望用于磁靶向、热疗和MRI诊断。 展开更多
关键词 柠檬酸 超顺磁性氧化铁 纳米粒 磁共振成像 磁靶向
下载PDF
头皮脑电图在迷走神经电刺激治疗难治性癫痫研究中的应用 被引量:10
3
作者 覃小雅 袁媛 +4 位作者 陈彦 廖建湘 林素芳 杨曌(综述) 李路明(审校) 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2020年第4期699-707,共9页
癫痫发作是由于大脑神经元过度放电的扩散而引起的,因此半个世纪以来脑电图(EEG)一直是科学家研究癫痫以及评估癫痫治疗的重要工具。本文综述了近30年头皮EEG在迷走神经电刺激(VNS)治疗难治性癫痫的临床研究中的应用,重点从基于EEG特征... 癫痫发作是由于大脑神经元过度放电的扩散而引起的,因此半个世纪以来脑电图(EEG)一直是科学家研究癫痫以及评估癫痫治疗的重要工具。本文综述了近30年头皮EEG在迷走神经电刺激(VNS)治疗难治性癫痫的临床研究中的应用,重点从基于EEG特征预测VNS对药物难治性癫痫的疗效和VNS治疗对药物难治性癫痫患者的EEG影响两方面展开介绍,并阐述相关VNS治疗机制。其中,针对基于EEG特征预测VNS疗效方面,介绍了痫样放电变化、慢皮质电位变化极性、脑电对称性以及脑电功率谱变化等特征指标与疗效的关系;针对VNS治疗对患者EEG特征的影响方面,介绍了VNS治疗后痫样放电、功率谱、同步性、脑网络以及事件相关电位P300振幅的变化等影响。尽管目前的研究尚未确定具有代表性的、可用于临床推广的EEG标志物,本综述可为大宗案例的前瞻性研究奠定基础,有利于指导EEG应用于VNS的临床治疗,为预测、评估VNS疗效以及理解VNS治疗机制提供思路,具有重要的临床价值和科学意义。 展开更多
关键词 迷走神经电刺激 脑电图 疗效预测 疗效评估 治疗机制
原文传递
Development and validation of a liquid chromatography with tandem mass spectrometry(LC-MS/MS) method for the determination of sunitinib in rabbit plasma 被引量:1
4
作者 杨勇杰 李佳林 +6 位作者 王欢 覃小雅 卢晓静 张兰馨 张悦 顾梦洁 范田园 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2015年第11期721-725,共5页
A liquid chromatography with tandem mass spectrometry(LC-MS/MS) method has been developed and validated for the measurement of sunitinib in rabbit plasma. After protein precipitation with acetonitrile, samples were ... A liquid chromatography with tandem mass spectrometry(LC-MS/MS) method has been developed and validated for the measurement of sunitinib in rabbit plasma. After protein precipitation with acetonitrile, samples were analyzed on a Zorbax Extend-C18 column(150 mm×4.6 mm, 5μm). The mobile phase consisted of a mixture of acetonitrile and deionized water(containing 0.05% formic acid) at a ratio of 27:73(v/v), and the flow rate was set at 0.8 mL /min. The column temperature was maintained at 30 oC. The LC eluate was detected by an electrospray ionization(ESI) source operated in the positive ion mode, and quantification was conducted using MRM of the transitions m/z 399.24→283.01 and m/z 415.19→178.00 for sunitinib and internal standard(IS, diltiazem hydrochloride), respectively. The calibration curve was linear in the range of 2–600 ng/m L. The lower limit of quantification was 2 ng/mL. The method also exhibited satisfactory results in terms of sensitivity, specificity, accuracy(with relative error ranging from –4.0% to 1.1%), precision(with intra- and inter-day relative standard deviations ranging from 2.8% to 9.5%), matrix effect, recovery as well as stability. Taken together, our newly developed method was reliable to monitor sunitinib concentrations in rabbit plasma. 展开更多
关键词 SUNITINIB LC-MS/MS Rabbit plasma
原文传递
Microwave-assisted preparation and in vitro characterizations of doped superparamagnetic ferrite nanoclusters 被引量:1
5
作者 Xiaoxin Liu Xiaoya Qin +1 位作者 Ziyuan Li Tianyuan Fan 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2020年第3期153-160,共8页
In order to obtain nanomaterials with superparamagnetism and high saturation magnetization, Mn-doped or Zn-doped superparamagnetic ferrite nanoclusters(Mn-FNs or Zn-FNs) were prepared by microwave-assisted solvotherma... In order to obtain nanomaterials with superparamagnetism and high saturation magnetization, Mn-doped or Zn-doped superparamagnetic ferrite nanoclusters(Mn-FNs or Zn-FNs) were prepared by microwave-assisted solvothermal method in this study. Preliminary investigations were performed by transmission electron microscopy(TEM) and dynamic light scattering(DLS) instrument to observe the morphology and measure the particle size, respectively. Afterwards, Zn-FNs were chosen to be further characterized in vitro due to their better morphology and dispersity than Mn-FNs. The subsequent characterizations included crystalline phase, metal content and magnetic properties by X-ray diffractometer(XRD), inductively coupled plasma-mass spectrometry(ICP-MS) and vibrating sample magnetometer(VSM), respectively. The results showed that Zn-FNs had a cluster-like structure assembled by multiple nanoparticles. Zn-FNs were spherical in shape with good dispersity and relatively uniform particle size. Zn was successfully doped in Zn-FNs which demonstrated spinel structure and excellent magnetic properties. Therefore, Zn-FNs had a favorable application prospect as a new type of magnetic nanomaterial. 展开更多
关键词 Microwave-assisted solvothermal method Doped ferrite nanoclusters In vitro characterizations
原文传递
A simple and sensitive gradient elution liquid chromatography with tandem mass spectrometry(LC-MS/MS) method for the quantification of doxorubicin in rabbit plasma
6
作者 Jialin Li Liying Guo +1 位作者 Xiaoya Qin Tianyuan Fan 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2016年第8期576-581,共6页
In the present study, we developed and validated a simple and sensitive gradient elution liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of doxorubicin in rabbit plasma. Daunorubi... In the present study, we developed and validated a simple and sensitive gradient elution liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of doxorubicin in rabbit plasma. Daunorubicin was used as an internal standard (IS). The doxorubicin and IS were extracted with ethyl acetate from plasma samples. The chromatographic separations were achieved on a C18 column (2.1 mm×50 mm, 2.5μm) configured with a C18 guard column (2.1 mm×10 mm, 2.5 μm). The mobile phase of 0.1% formic acid-water solution and acetonitrile was delivered using a gradient elution program at a flow rate of 0.4 mL/min. The temperature for column was maintained at 40 ℃. The electrospray ionization (ESI) source was operated in the positive ion mode, and the quantification was conducted using multiple reaction monitoring (MRM) of the transitions m/z 544.07→396.96 and m/z 528.06→321.05 for doxorubicin and IS, respectively. The calibration curve of doxorubicin was linear (r 〉 0.999) within the range of 2-600 ng/mL. The lower limit of quantification was 2 ng/mL. The relative errors of intra-day and inter-day accuracies ranged from -2.48% to 0.18% and from -3.78% to 1.94%, respectively. The relative standard deviations of intra-day and inter-day precisions were less than 8.65% and 6.41%, respectively. The method exhibited satisfactory results in terms of specificity, sensitivity, matrix effect, recovery and stability. The newly developed LC-MS/MS method was reliable to monitor doxorubicin concentrations in rabbit plasma. 展开更多
关键词 DOXORUBICIN LC-MS/MS Gradient elution Rabbit plasma
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部