The development of super-resolution fluorescence microscopy is very essential for understanding the physical and biological fundamentals at nanometer scale.However,to date most super-resolution modalities require eith...The development of super-resolution fluorescence microscopy is very essential for understanding the physical and biological fundamentals at nanometer scale.However,to date most super-resolution modalities require either complicated/costly purpose-built systems such as multiple-beam architectures or complex post-processing procedures with intrinsic artifacts.Achieving three-dimensional(3D)or multi-channel sub-diffraction microscopic imaging using a simple method remains a challenging and struggling task.Herein,we proposed 3D highly-nonlinear super-resolution microscopy using a singlebeam excitation strategy,and the microscopy principle was modelled and studied based on the ultrahigh nonlinearity enabled by photon avalanches.According to the simulation,the point spread function of highly nonlinear microscopy is switchable among different modes and can shrink three-dimensionally to sub-diffraction scale at the photon avalanche mode.Experimentally,we demonstrated 3D optical nanoscopy assisted with huge optical nonlinearities in a simple laser scanning configuration,achieving a lateral resolution down to 58 nm(λ/14)and an axial resolution down to 185 nm(λ/5)with one single beam of low-power,continuous-wave,near-infrared laser.We further extended the photon avalanche effect to many other emitters to develop multi-color photon avalanching nanoprobes based on migrating photon avalanche mechanism,which enables us to implement single-beam dual-color sub-diffraction super-resolution microscopic imaging.展开更多
基金supported by the National Natural Science Foundation of China(62335008,62122028,62105106,and 11974123)Guangdong Basic and Applied Basic Research Foundation(2023B1515040018,2022A1515011395,and 2019A050510037)+2 种基金the Guangdong Provincial Science Fund for Distinguished Yong Scholars(2018B030306015)Guangzhou Basic and Applied Basic Research Foundation(202201010376)China Postdoctoral Science Foundation(2023T160237 and 2021M691089).
文摘The development of super-resolution fluorescence microscopy is very essential for understanding the physical and biological fundamentals at nanometer scale.However,to date most super-resolution modalities require either complicated/costly purpose-built systems such as multiple-beam architectures or complex post-processing procedures with intrinsic artifacts.Achieving three-dimensional(3D)or multi-channel sub-diffraction microscopic imaging using a simple method remains a challenging and struggling task.Herein,we proposed 3D highly-nonlinear super-resolution microscopy using a singlebeam excitation strategy,and the microscopy principle was modelled and studied based on the ultrahigh nonlinearity enabled by photon avalanches.According to the simulation,the point spread function of highly nonlinear microscopy is switchable among different modes and can shrink three-dimensionally to sub-diffraction scale at the photon avalanche mode.Experimentally,we demonstrated 3D optical nanoscopy assisted with huge optical nonlinearities in a simple laser scanning configuration,achieving a lateral resolution down to 58 nm(λ/14)and an axial resolution down to 185 nm(λ/5)with one single beam of low-power,continuous-wave,near-infrared laser.We further extended the photon avalanche effect to many other emitters to develop multi-color photon avalanching nanoprobes based on migrating photon avalanche mechanism,which enables us to implement single-beam dual-color sub-diffraction super-resolution microscopic imaging.
基金financially supported by the Key R&D Program of Guangzhou (202007020003)the National Natural Science Foundation of China (62075063, 51772101 and 51872095)+2 种基金the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (2017BT01X137)the Natural Science Foundation of Guangdong Province (2019B030301003)the State Key Lab of Luminescent Materials and Devices,South China University of Technology
