Carnoy′s fluid and neutral buffered formalin(NBF)have been proved to be good fixatives for preservation of mast cells in pig,cattle and sheep except NBF blocked staining of most porcine mast cells,especially thos...Carnoy′s fluid and neutral buffered formalin(NBF)have been proved to be good fixatives for preservation of mast cells in pig,cattle and sheep except NBF blocked staining of most porcine mast cells,especially those located in intestinal mucosa(MMC)and in thymus medulla(TMMC). Both toluidine blue and Alcian blue were the excellent stains generally,but Alcian blue stained more porcine mast cells than did toluidine blue( P <0 01). Staining with toluidine blue of a wide pH range(from 0 1 to 7 0)showed that porcine mast cells were not very pH dependent,but the dye at pH 0 5 seemed to have the strongest affinity for all mast cells in pigs and it was also suitable for bovine and ovine mast cell staining. In the three species,unlike in rodents,the Alcian blue method did not distinguish between mast cells in the intestinal mucosa(MMC)and those in the connective tissue of the intestinal submucosa,tongue and skin(CTMC). Porcine CTMC,but not MMC,fluoresced strongly when stained with berberine sulphate or with a mixture of berberine sulphate and acridine orange. It suggested that porcine CTMC contained heparin proteoglycan.展开更多
蕨属值物(Pteridium scopoli)是一类分布极为广泛而又十分古老的植物.其代表种欧洲蕨(Pteridium aguilinum L Kuhn)为世界广布种,简称蕨.它至少有8个变种,其中又以主要分布于欧洲及非洲的原变种(var.aguilinum)及主要分布于北美、欧洲...蕨属值物(Pteridium scopoli)是一类分布极为广泛而又十分古老的植物.其代表种欧洲蕨(Pteridium aguilinum L Kuhn)为世界广布种,简称蕨.它至少有8个变种,其中又以主要分布于欧洲及非洲的原变种(var.aguilinum)及主要分布于北美、欧洲及亚洲北部的斜羽变种(var.laliusculum)最为重要.现有的研究资料业已证明,蕨对动物具有肯定的毒性与致癌性.它能引起马等单胃动物的硫胺素缺乏症。展开更多
文摘Carnoy′s fluid and neutral buffered formalin(NBF)have been proved to be good fixatives for preservation of mast cells in pig,cattle and sheep except NBF blocked staining of most porcine mast cells,especially those located in intestinal mucosa(MMC)and in thymus medulla(TMMC). Both toluidine blue and Alcian blue were the excellent stains generally,but Alcian blue stained more porcine mast cells than did toluidine blue( P <0 01). Staining with toluidine blue of a wide pH range(from 0 1 to 7 0)showed that porcine mast cells were not very pH dependent,but the dye at pH 0 5 seemed to have the strongest affinity for all mast cells in pigs and it was also suitable for bovine and ovine mast cell staining. In the three species,unlike in rodents,the Alcian blue method did not distinguish between mast cells in the intestinal mucosa(MMC)and those in the connective tissue of the intestinal submucosa,tongue and skin(CTMC). Porcine CTMC,but not MMC,fluoresced strongly when stained with berberine sulphate or with a mixture of berberine sulphate and acridine orange. It suggested that porcine CTMC contained heparin proteoglycan.
文摘蕨属值物(Pteridium scopoli)是一类分布极为广泛而又十分古老的植物.其代表种欧洲蕨(Pteridium aguilinum L Kuhn)为世界广布种,简称蕨.它至少有8个变种,其中又以主要分布于欧洲及非洲的原变种(var.aguilinum)及主要分布于北美、欧洲及亚洲北部的斜羽变种(var.laliusculum)最为重要.现有的研究资料业已证明,蕨对动物具有肯定的毒性与致癌性.它能引起马等单胃动物的硫胺素缺乏症。
