Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharid...Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharides and proteins,and the quality and quantity of DNA extracted were reliably characterized.In addition,the reaction composition and amplification program for RAPD analysis were developed with GeneAmp PCR System 2700 (Applied Biosystem Co.).This study provided a technological basis for future molecular studies in rattan and the related species.展开更多
This paper dealed with the differences between albino and normal seedlings of Calamus nambarensis var.xishuangbanensis in terms of growth and photosynthesis.Thirteen families among 22 families were found to have one t...This paper dealed with the differences between albino and normal seedlings of Calamus nambarensis var.xishuangbanensis in terms of growth and photosynthesis.Thirteen families among 22 families were found to have one to three albinos,the percent of which varying from 0.4 to 2.2 percents,while the percent of albinos among all 22 families was about 0.7 percent.The number of albino leaves and the length of longest leaves were 1.9±0.3 and 22.32±3.49 cm respectively, which were less than that of 2.0±0.0 and 26.09±3.26 cm of normal seedlings,accounting for 95% and 85% of normal seedlings.The albinos hadn’t chlorophyll a,but some chlorophyll b.The total content of chlorophyll was 0.02 mg·g-1FW, much less than that of 8.15 mg·g-1FW of normal seedlings, accounting for 0.2 percent.Whether photosynthesis active radiation（PAR） was 800 μmol·m-2·s-1 or （1 600） μmol·m-2·s-1,the photosynthetic rate of albinos was less than that of normal seedlings,accounting for about 20 percents.The data of net photosynthetic rate of albino evaluatde by the authors were also展开更多
This paper deals with tubeseedling afforestation of Calamus simplicifolius. The survival rate of tubeseedlings planted in arboretum and Duadanga grandiflora forest is not lower than that of normal seedling...This paper deals with tubeseedling afforestation of Calamus simplicifolius. The survival rate of tubeseedlings planted in arboretum and Duadanga grandiflora forest is not lower than that of normal seedlings. That indicates the afforestation requirements of tubeseedlings, such as site selection, site preparation, shading trees and planting, can be similar to those of normal seedlings. The survival rate of tubeseedlings planted in arboretum, teak forest, Michelia macclurei forest, Duadanga grandiflora forest and natural secondary forest can be higher than ninety percent. The afforestation results of clump seedlings show that the survival rate of shoots in the clump is relative to their initial height before afforestation. The highest shoot in clumps has the highest survival rate. In clump seedlings, individual shoots with a height lower than 20 cm have very low afforestation survival rate. There is eminent difference among the height growth of different tissue culture families. This indicates the selection of elite family or plus plant can enhance the production of rattan forests. Experiment results also show that plants developed from tubeseedlings have stronger suckering ability and can germinate suckers one or two years earlier than those developed from normal seedlings. This phenomenon needs more research and more attention should be paid on the remnant impact of plant growth regulators applied in proliferation culture.展开更多
文摘Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharides and proteins,and the quality and quantity of DNA extracted were reliably characterized.In addition,the reaction composition and amplification program for RAPD analysis were developed with GeneAmp PCR System 2700 (Applied Biosystem Co.).This study provided a technological basis for future molecular studies in rattan and the related species.
文摘This paper dealed with the differences between albino and normal seedlings of Calamus nambarensis var.xishuangbanensis in terms of growth and photosynthesis.Thirteen families among 22 families were found to have one to three albinos,the percent of which varying from 0.4 to 2.2 percents,while the percent of albinos among all 22 families was about 0.7 percent.The number of albino leaves and the length of longest leaves were 1.9±0.3 and 22.32±3.49 cm respectively, which were less than that of 2.0±0.0 and 26.09±3.26 cm of normal seedlings,accounting for 95% and 85% of normal seedlings.The albinos hadn’t chlorophyll a,but some chlorophyll b.The total content of chlorophyll was 0.02 mg·g-1FW, much less than that of 8.15 mg·g-1FW of normal seedlings, accounting for 0.2 percent.Whether photosynthesis active radiation（PAR） was 800 μmol·m-2·s-1 or （1 600） μmol·m-2·s-1,the photosynthetic rate of albinos was less than that of normal seedlings,accounting for about 20 percents.The data of net photosynthetic rate of albino evaluatde by the authors were also
文摘This paper deals with tubeseedling afforestation of Calamus simplicifolius. The survival rate of tubeseedlings planted in arboretum and Duadanga grandiflora forest is not lower than that of normal seedlings. That indicates the afforestation requirements of tubeseedlings, such as site selection, site preparation, shading trees and planting, can be similar to those of normal seedlings. The survival rate of tubeseedlings planted in arboretum, teak forest, Michelia macclurei forest, Duadanga grandiflora forest and natural secondary forest can be higher than ninety percent. The afforestation results of clump seedlings show that the survival rate of shoots in the clump is relative to their initial height before afforestation. The highest shoot in clumps has the highest survival rate. In clump seedlings, individual shoots with a height lower than 20 cm have very low afforestation survival rate. There is eminent difference among the height growth of different tissue culture families. This indicates the selection of elite family or plus plant can enhance the production of rattan forests. Experiment results also show that plants developed from tubeseedlings have stronger suckering ability and can germinate suckers one or two years earlier than those developed from normal seedlings. This phenomenon needs more research and more attention should be paid on the remnant impact of plant growth regulators applied in proliferation culture.
