目的:探讨补肾活血中药联合经皮穴位电刺激改善辅助生殖技术助孕治疗中发生反复移植失败(RIF)的患者妊娠结局。方法:选择2015年至2019年在长沙市妇幼保健院生殖中心进行体外受精-胚胎移植(IVF-ET)助孕RIF患者169例,随机分为对照组(雌激...目的:探讨补肾活血中药联合经皮穴位电刺激改善辅助生殖技术助孕治疗中发生反复移植失败(RIF)的患者妊娠结局。方法:选择2015年至2019年在长沙市妇幼保健院生殖中心进行体外受精-胚胎移植(IVF-ET)助孕RIF患者169例,随机分为对照组(雌激素组)40例,观察组1(中药+雌激素组)35例,观察组2(经皮穴位电刺激治疗+雌激素组)43例,观察组3(中药联合经皮穴位电刺激+雌激素组)51例,监测子宫内膜厚度、雌二醇水平、转化日前雌二醇用量及移植后14天血人绒毛膜促性腺激素(hCG)阳性率及临床妊娠率。结果:治疗7天后,各组间观察指标差异无统计学意义(P>0.05)。转化日观察组3子宫内膜厚度明显高于对照组及观察组2(9.24±1.54 mm vs 8.35±0.99,9.24±1.54 mm vs 8.31±0.77 mm),差异有统计学意义(P<0.05)。转化日观察组1及观察组3雌二醇水平明显高于对照组(933.09±238.35 pmol/L vs 825.32±153.59 pmol/L,964.49±181.27 pmol/L vs 825.32±153.59 pmol/L),差异有统计学意义(P<0.05)。观察组3及观察组1雌激素使用量低于其他两组,但差异无统计学意义(P>0.05)。观察组3与对照组相比移植14天后血hCG阳性率、临床妊娠率明显增高,差异有统计学意义(P<0.05)。结论:中药联合经皮穴位电刺激及雌激素治疗可以改善RIF患者子宫内膜容受性,较对照组在移植14天后血hCG阳性率、临床妊娠率明显增加。展开更多
Objective： To explore the action mechanism of electroacupuncture （EA） in improving the gastric motility of rats with diabetic gastroparesis （DGP）. Methods： Forty-eight healthy Sprague-Dawley （SD） rats were ran...Objective： To explore the action mechanism of electroacupuncture （EA） in improving the gastric motility of rats with diabetic gastroparesis （DGP）. Methods： Forty-eight healthy Sprague-Dawley （SD） rats were randomly assigned into four groups： a normal group （group A）, a model group （group B）, a group of EA at acupoints （group C）, and a group of EA at non-acupoints （group D）, 12 rats in each group. The animal model of DGP was established by intraperitoneal injection of streptozotocin （STZ） plus high glucose and fat diet. The blood glucose, urine glucose and gastric emptying rate （GER） were observed. The content of insulin （INS） in serum and ghrelin in gastric antrum tissue were detected by enzyme linked immunoassay （ELISA）. The expression of growth hormone secretagogue receptor mRNA （GHSR mRNA） in gastric antrum tissue was detected by real-time fluorescent quantitative polymerase chain reaction （PCR）. Results：Compared with group A, blood glucose and urine glucose increased significantly （P〈0.01）, GER, content of serum insulin, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue decreased significantly （P〈0.05 orP〈0.01） in group B. Compared with group B, blood glucose and urine glucose decreased significantly （P〈0.05）, GER, the content of insulin in serum, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue increased significantly （P〈0.05 orP〈0.01） in group C. Conclusion： EA at acupoints can down-regulate the content of blood and urine sugar, and promote gastric emptying, which is possibly related to the regulation of serum insulin, and the expressions of ghrelin and GHSR mRNA in gastric antrum.展开更多
基金国家自然科学基金资助项目(81403487)-从胃窦ICC的起搏机制探讨电针对糖尿病胃轻瘫大鼠胃运动的影响湖南省高校创新平台开放基金项目(12 K 088)-基于ghrelin-GHSR-Ca2+信号通路探讨电针对DGP模型大鼠胃运动的影响湖南省教育厅优秀青年项目(14B128)-电针对糖尿病胃轻瘫大鼠胃窦ICC形态及起搏功能的影响
文摘目的:探讨补肾活血中药联合经皮穴位电刺激改善辅助生殖技术助孕治疗中发生反复移植失败(RIF)的患者妊娠结局。方法:选择2015年至2019年在长沙市妇幼保健院生殖中心进行体外受精-胚胎移植(IVF-ET)助孕RIF患者169例,随机分为对照组(雌激素组)40例,观察组1(中药+雌激素组)35例,观察组2(经皮穴位电刺激治疗+雌激素组)43例,观察组3(中药联合经皮穴位电刺激+雌激素组)51例,监测子宫内膜厚度、雌二醇水平、转化日前雌二醇用量及移植后14天血人绒毛膜促性腺激素(hCG)阳性率及临床妊娠率。结果:治疗7天后,各组间观察指标差异无统计学意义(P>0.05)。转化日观察组3子宫内膜厚度明显高于对照组及观察组2(9.24±1.54 mm vs 8.35±0.99,9.24±1.54 mm vs 8.31±0.77 mm),差异有统计学意义(P<0.05)。转化日观察组1及观察组3雌二醇水平明显高于对照组(933.09±238.35 pmol/L vs 825.32±153.59 pmol/L,964.49±181.27 pmol/L vs 825.32±153.59 pmol/L),差异有统计学意义(P<0.05)。观察组3及观察组1雌激素使用量低于其他两组,但差异无统计学意义(P>0.05)。观察组3与对照组相比移植14天后血hCG阳性率、临床妊娠率明显增高,差异有统计学意义(P<0.05)。结论:中药联合经皮穴位电刺激及雌激素治疗可以改善RIF患者子宫内膜容受性,较对照组在移植14天后血hCG阳性率、临床妊娠率明显增加。
基金supported by National Natural Science Foundation of China No.81403487Youth Fund of Hunan Provincial Education Department No.14B128Open Fund Project of Hunan University Innovation Platform No.12K088~~
文摘Objective： To explore the action mechanism of electroacupuncture （EA） in improving the gastric motility of rats with diabetic gastroparesis （DGP）. Methods： Forty-eight healthy Sprague-Dawley （SD） rats were randomly assigned into four groups： a normal group （group A）, a model group （group B）, a group of EA at acupoints （group C）, and a group of EA at non-acupoints （group D）, 12 rats in each group. The animal model of DGP was established by intraperitoneal injection of streptozotocin （STZ） plus high glucose and fat diet. The blood glucose, urine glucose and gastric emptying rate （GER） were observed. The content of insulin （INS） in serum and ghrelin in gastric antrum tissue were detected by enzyme linked immunoassay （ELISA）. The expression of growth hormone secretagogue receptor mRNA （GHSR mRNA） in gastric antrum tissue was detected by real-time fluorescent quantitative polymerase chain reaction （PCR）. Results：Compared with group A, blood glucose and urine glucose increased significantly （P〈0.01）, GER, content of serum insulin, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue decreased significantly （P〈0.05 orP〈0.01） in group B. Compared with group B, blood glucose and urine glucose decreased significantly （P〈0.05）, GER, the content of insulin in serum, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue increased significantly （P〈0.05 orP〈0.01） in group C. Conclusion： EA at acupoints can down-regulate the content of blood and urine sugar, and promote gastric emptying, which is possibly related to the regulation of serum insulin, and the expressions of ghrelin and GHSR mRNA in gastric antrum.
