The good growth behavior was achieved for a Trichoplusia ni cell line,called Tn 5B1 4(Tn5)cells,in suspension culture by the use of IC-SFM serum free medium developed by our lab.This medium,combined with the specially...The good growth behavior was achieved for a Trichoplusia ni cell line,called Tn 5B1 4(Tn5)cells,in suspension culture by the use of IC-SFM serum free medium developed by our lab.This medium,combined with the specially designed spinner flask with relatively good culture environment,could support the fragile Tn5 cells to grow well even in low inoculum density.The effect of inner designs of spinner flasks on cellular growth and metabolism was discussed on the basis of this culture system.展开更多
The oxygen uptake rate( OUR ) during the cultivation of Vero cells in 1.5L CelliGen bioreactor was on line determined using the dynamic method.The results showed that the cell growth and metabolic state during the exp...The oxygen uptake rate( OUR ) during the cultivation of Vero cells in 1.5L CelliGen bioreactor was on line determined using the dynamic method.The results showed that the cell growth and metabolic state during the exponential growth phase was lineally related to the OUR .This implies that the on line measurement of OUR can be used to promptly monitor the physiological state of cultured cells and to efficiently avoid contamination because of frequent sampling in the large scale cultivation of mammalian cells.展开更多
文摘The good growth behavior was achieved for a Trichoplusia ni cell line,called Tn 5B1 4(Tn5)cells,in suspension culture by the use of IC-SFM serum free medium developed by our lab.This medium,combined with the specially designed spinner flask with relatively good culture environment,could support the fragile Tn5 cells to grow well even in low inoculum density.The effect of inner designs of spinner flasks on cellular growth and metabolism was discussed on the basis of this culture system.
文摘The oxygen uptake rate( OUR ) during the cultivation of Vero cells in 1.5L CelliGen bioreactor was on line determined using the dynamic method.The results showed that the cell growth and metabolic state during the exponential growth phase was lineally related to the OUR .This implies that the on line measurement of OUR can be used to promptly monitor the physiological state of cultured cells and to efficiently avoid contamination because of frequent sampling in the large scale cultivation of mammalian cells.
