目的基于CYP2J3/EETs介导脂肪酸代谢探讨化瘀祛痰方防治血脂异常的机制。方法32只SPF级SD大鼠分为空白对照组、模型组、化瘀祛痰方组、辛伐他汀组,每组8只。除空白对照组外,其他各组给予高脂饲料喂饲,建立高脂血症模型。于第13周开始,...目的基于CYP2J3/EETs介导脂肪酸代谢探讨化瘀祛痰方防治血脂异常的机制。方法32只SPF级SD大鼠分为空白对照组、模型组、化瘀祛痰方组、辛伐他汀组,每组8只。除空白对照组外,其他各组给予高脂饲料喂饲,建立高脂血症模型。于第13周开始,化瘀祛痰方组给予化瘀祛痰方灌胃,辛伐他汀组给予辛伐他汀灌胃,空白对照组、模型组给予相应体积的生理盐水灌胃,每日1次,灌胃4周。采用全自动生化分析仪检测各组大鼠血清中总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)、低密度脂蛋白胆固醇(Low density lipoprotein cholesterol,LDL-C)及高密度脂蛋白胆固醇(High density lipoprotein cholesterol,HDL-C)水平;HE染色观察肝脏组织病理变化;Elisa检测各组大鼠肝脏14,15-环氧二十碳三烯酸(14,15-epoxyeicosatrienoic acid,14,15-EET)水平;Real time RT-PCR检测肝脏组织细胞色素P450表氧化酶2J3(Cytochrome P450 oxidase 2J3,CYP2J3)、肉毒碱棕榈酰转移酶1(Carnitine palmitoyltransferase-1,CPT-1)mRNA水平;Western blot法检测肝脏组织CYP2J3、腺苷酸活化蛋白激酶(AMP-actived protein kinase,AMPK)、磷酸化腺苷酸活化蛋白激酶(p-AMPK)、乙酰辅酶A羧化酶(Acetyl CoA Carboxylase,ACC)、磷酸化乙酰辅酶A羧化酶(p-ACC)、CPT-1蛋白水平。结果与空白对照组比较,模型组大鼠血清TC、TG和LDL-C水平显著升高(P<0.01),HDL-C水平显著降低(P<0.05);肝脏脂质沉积显著,可见大量脂滴;肝脏组织内14,15-EET含量显著降低(P<0.01);肝脏CYP2J3、CPT-1 mRNA和蛋白表达水平显著降低(P<0.01),p-AMPK/AMPK、p-ACC/ACC显著降低(P<0.01)。与模型组比较,经化瘀祛痰方及辛伐他汀干预后,大鼠血清TC、TG和LDL-C水平显著降低(P<0.05或P<0.01),HDL-C水平显著升高(P<0.05);肝脏脂质沉积情况明显改善,脂滴数明显减少;肝脏组织内14,15-EET水平显著升高(P<0.01);肝脏CYP2J3、CPT-1 mRNA和蛋白表达水平显著升高(P<0.05或P<0.01),p-AMPK/AMPK、p-ACC/ACC显著升高(P<0.05或P<0.01)。结论化瘀祛痰方可有效改善高脂血症大鼠血脂异常,减轻肝脏脂质沉积情况,其机制可能与CYP2J3/EETs介导脂肪酸代谢密切相关。展开更多
糖尿病肾病(DN)作为糖尿病微血管并发症之一,其发生和发展受到了医学研究者和临床医生的广泛关注。糖肾康饮,是中医治疗DN的经典药方,该方由黑龙江省名中医马建教授创制,针对DNIII期患者阴虚内热、耗气伤血以及由气虚引发瘀血的病理特征...糖尿病肾病(DN)作为糖尿病微血管并发症之一,其发生和发展受到了医学研究者和临床医生的广泛关注。糖肾康饮,是中医治疗DN的经典药方,该方由黑龙江省名中医马建教授创制,针对DNIII期患者阴虚内热、耗气伤血以及由气虚引发瘀血的病理特征,以益气滋阴和化瘀活血为治疗原则所立,对DN及其相关症状有良好的治疗效果。本文通过深入探讨糖肾康饮的组方分析、药理研究及临床应用,为糖肾康饮在DN的治疗应用中提供更加全面的理论支持和科学依据。As one of the microvascular complications of diabetes, the occurrence and development of diabetes nephropathy (DN) has received extensive attention from medical researchers and clinicians. Tangshenkang Decoction is a classic prescription for treating DN in traditional Chinese medicine. It was created by Professor Ma Jian, a renowned traditional Chinese medicine practitioner in Heilongjiang Province. It focuses on the pathological characteristics of Yin deficiency, internal heat, qi consumption, blood injury, and blood stasis caused by Qi deficiency in patients with DN III, and is based on the principles of supplementing qi, nourishing yin, and promoting blood stasis. It has a good therapeutic effect on DN and its related symptoms. This article provides more comprehensive theoretical support and scientific basis for the treatment and application of Tangshenkang Decoction in DN by deeply exploring the composition analysis, pharmacological research, and clinical application of Tangshenkang Decoction.展开更多
文摘目的基于CYP2J3/EETs介导脂肪酸代谢探讨化瘀祛痰方防治血脂异常的机制。方法32只SPF级SD大鼠分为空白对照组、模型组、化瘀祛痰方组、辛伐他汀组,每组8只。除空白对照组外,其他各组给予高脂饲料喂饲,建立高脂血症模型。于第13周开始,化瘀祛痰方组给予化瘀祛痰方灌胃,辛伐他汀组给予辛伐他汀灌胃,空白对照组、模型组给予相应体积的生理盐水灌胃,每日1次,灌胃4周。采用全自动生化分析仪检测各组大鼠血清中总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)、低密度脂蛋白胆固醇(Low density lipoprotein cholesterol,LDL-C)及高密度脂蛋白胆固醇(High density lipoprotein cholesterol,HDL-C)水平;HE染色观察肝脏组织病理变化;Elisa检测各组大鼠肝脏14,15-环氧二十碳三烯酸(14,15-epoxyeicosatrienoic acid,14,15-EET)水平;Real time RT-PCR检测肝脏组织细胞色素P450表氧化酶2J3(Cytochrome P450 oxidase 2J3,CYP2J3)、肉毒碱棕榈酰转移酶1(Carnitine palmitoyltransferase-1,CPT-1)mRNA水平;Western blot法检测肝脏组织CYP2J3、腺苷酸活化蛋白激酶(AMP-actived protein kinase,AMPK)、磷酸化腺苷酸活化蛋白激酶(p-AMPK)、乙酰辅酶A羧化酶(Acetyl CoA Carboxylase,ACC)、磷酸化乙酰辅酶A羧化酶(p-ACC)、CPT-1蛋白水平。结果与空白对照组比较,模型组大鼠血清TC、TG和LDL-C水平显著升高(P<0.01),HDL-C水平显著降低(P<0.05);肝脏脂质沉积显著,可见大量脂滴;肝脏组织内14,15-EET含量显著降低(P<0.01);肝脏CYP2J3、CPT-1 mRNA和蛋白表达水平显著降低(P<0.01),p-AMPK/AMPK、p-ACC/ACC显著降低(P<0.01)。与模型组比较,经化瘀祛痰方及辛伐他汀干预后,大鼠血清TC、TG和LDL-C水平显著降低(P<0.05或P<0.01),HDL-C水平显著升高(P<0.05);肝脏脂质沉积情况明显改善,脂滴数明显减少;肝脏组织内14,15-EET水平显著升高(P<0.01);肝脏CYP2J3、CPT-1 mRNA和蛋白表达水平显著升高(P<0.05或P<0.01),p-AMPK/AMPK、p-ACC/ACC显著升高(P<0.05或P<0.01)。结论化瘀祛痰方可有效改善高脂血症大鼠血脂异常,减轻肝脏脂质沉积情况,其机制可能与CYP2J3/EETs介导脂肪酸代谢密切相关。
文摘糖尿病肾病(DN)作为糖尿病微血管并发症之一,其发生和发展受到了医学研究者和临床医生的广泛关注。糖肾康饮,是中医治疗DN的经典药方,该方由黑龙江省名中医马建教授创制,针对DNIII期患者阴虚内热、耗气伤血以及由气虚引发瘀血的病理特征,以益气滋阴和化瘀活血为治疗原则所立,对DN及其相关症状有良好的治疗效果。本文通过深入探讨糖肾康饮的组方分析、药理研究及临床应用,为糖肾康饮在DN的治疗应用中提供更加全面的理论支持和科学依据。As one of the microvascular complications of diabetes, the occurrence and development of diabetes nephropathy (DN) has received extensive attention from medical researchers and clinicians. Tangshenkang Decoction is a classic prescription for treating DN in traditional Chinese medicine. It was created by Professor Ma Jian, a renowned traditional Chinese medicine practitioner in Heilongjiang Province. It focuses on the pathological characteristics of Yin deficiency, internal heat, qi consumption, blood injury, and blood stasis caused by Qi deficiency in patients with DN III, and is based on the principles of supplementing qi, nourishing yin, and promoting blood stasis. It has a good therapeutic effect on DN and its related symptoms. This article provides more comprehensive theoretical support and scientific basis for the treatment and application of Tangshenkang Decoction in DN by deeply exploring the composition analysis, pharmacological research, and clinical application of Tangshenkang Decoction.
