This paper reports for the first time an experiment designed in which synthesized α cyano ferulic acid used as a new matrix for the analysis of cytochrome c, trypsin, bovine serum albumin, β cyclodextrin, dextrans a...This paper reports for the first time an experiment designed in which synthesized α cyano ferulic acid used as a new matrix for the analysis of cytochrome c, trypsin, bovine serum albumin, β cyclodextrin, dextrans and DNA d(T) 18 . The results demonstrated that α cyano ferulic acid used as a new matrix could effectively desorb and ionize different kinds of samples, such as proteins and peptides, oligosaccharides, polysaccharides and oligonucleotides. In analysis of dextrans the new matrix gave better results when comparing with the normally used DHB. In analysis of DNA d(T) 18 , the sample could be desorbed and ionized more efficiently even without sample purification, which is normally needed when using 3 HPA as a matrix.展开更多
Four coumaric derivatives including 3-aminocoumarin, 3-hydroxycoumarin, 3-carhoxycoumarin and 4-methyl-7-hydroxycoumarin were tested as matrices for peptides and proteins analyses by matrix-assisted laser desorption/i...Four coumaric derivatives including 3-aminocoumarin, 3-hydroxycoumarin, 3-carhoxycoumarin and 4-methyl-7-hydroxycoumarin were tested as matrices for peptides and proteins analyses by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The experimental results showed that all these coumaric derivatives (dissolved in acetonitrile at the concentration of 0. 1mol/L) can desorb/ionize the peptide and protein samples effectively and high resolution MALDI-TOFMS spectra were obtained.展开更多
α-cyano-ferulic acid(CFA)and 2,5-dihydroxybenzoic acid (DHB)were used as matrices to determineβ-cyclodextrin, saccharide A from animal, saccharide B from plant an dextrans by MALDI-TOF-MS. The results showed that CA...α-cyano-ferulic acid(CFA)and 2,5-dihydroxybenzoic acid (DHB)were used as matrices to determineβ-cyclodextrin, saccharide A from animal, saccharide B from plant an dextrans by MALDI-TOF-MS. The results showed that CAF could make some analytes desorbed and ionized more easliy and getting better reproducibility than DHB.展开更多
文摘This paper reports for the first time an experiment designed in which synthesized α cyano ferulic acid used as a new matrix for the analysis of cytochrome c, trypsin, bovine serum albumin, β cyclodextrin, dextrans and DNA d(T) 18 . The results demonstrated that α cyano ferulic acid used as a new matrix could effectively desorb and ionize different kinds of samples, such as proteins and peptides, oligosaccharides, polysaccharides and oligonucleotides. In analysis of dextrans the new matrix gave better results when comparing with the normally used DHB. In analysis of DNA d(T) 18 , the sample could be desorbed and ionized more efficiently even without sample purification, which is normally needed when using 3 HPA as a matrix.
文摘Four coumaric derivatives including 3-aminocoumarin, 3-hydroxycoumarin, 3-carhoxycoumarin and 4-methyl-7-hydroxycoumarin were tested as matrices for peptides and proteins analyses by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The experimental results showed that all these coumaric derivatives (dissolved in acetonitrile at the concentration of 0. 1mol/L) can desorb/ionize the peptide and protein samples effectively and high resolution MALDI-TOFMS spectra were obtained.
文摘α-cyano-ferulic acid(CFA)and 2,5-dihydroxybenzoic acid (DHB)were used as matrices to determineβ-cyclodextrin, saccharide A from animal, saccharide B from plant an dextrans by MALDI-TOF-MS. The results showed that CAF could make some analytes desorbed and ionized more easliy and getting better reproducibility than DHB.
