加速康复夕卜科(enhanced recovery after surgery,ERAS)理念推行至今因能有效减轻应激反应,降低并发症,缩短住院时间及减少住院费用等,在多学科得到应用和普及。近年来,多项新技术、新理念的涌现使甲状腺外科得以蓬勃的发展。本文就现...加速康复夕卜科(enhanced recovery after surgery,ERAS)理念推行至今因能有效减轻应激反应,降低并发症,缩短住院时间及减少住院费用等,在多学科得到应用和普及。近年来,多项新技术、新理念的涌现使甲状腺外科得以蓬勃的发展。本文就现阶段甲状腺外科领域开展的精准临床评估技术、多路径手术技术、组织器官功能保护技术以及多学科团队协作理念等展开梳理,为更好地促进ERAS的开展奠定基础。展开更多
To construct a hepatic stellate cells (HSCs) subtracted cDNA library to find differentially expressed genes in normal mice and mice infected with Schistosoma japonicum (S. japonicum). Suppression subtractive hybridiza...To construct a hepatic stellate cells (HSCs) subtracted cDNA library to find differentially expressed genes in normal mice and mice infected with Schistosoma japonicum (S. japonicum). Suppression subtractive hybridization (SSH) was used. The cDNA fragments of normal mouse were compared to those of schistosoma-infected mice to find differentially expressed genes. Then differentially expressed cDNA fragments were directly inserted into T/A cloning vector to set up the subtractive library. Amplification of the library was carried out with transformation of DH5α. The amplified library contained more than 400 positive bacterial clones, which were then hybridized with forward and backward subtracted probes for differential screening. One hundred positive bacterial clones were randomly selected for sequencing and BLAST analysis. Finally, virtual Northern Blot confirmed such differential expression. The subtracted cDNA library of differentially expressed genes of HSCs was constructed successfully, the library is efficient and lays foundation for screening and cloning new and specific genes of schistosomiasis.展开更多
文摘加速康复夕卜科(enhanced recovery after surgery,ERAS)理念推行至今因能有效减轻应激反应,降低并发症,缩短住院时间及减少住院费用等,在多学科得到应用和普及。近年来,多项新技术、新理念的涌现使甲状腺外科得以蓬勃的发展。本文就现阶段甲状腺外科领域开展的精准临床评估技术、多路径手术技术、组织器官功能保护技术以及多学科团队协作理念等展开梳理,为更好地促进ERAS的开展奠定基础。
基金Project (No. 2001BA705B08) supported by the National Ten-yearKey Technologies R&D Program China
文摘To construct a hepatic stellate cells (HSCs) subtracted cDNA library to find differentially expressed genes in normal mice and mice infected with Schistosoma japonicum (S. japonicum). Suppression subtractive hybridization (SSH) was used. The cDNA fragments of normal mouse were compared to those of schistosoma-infected mice to find differentially expressed genes. Then differentially expressed cDNA fragments were directly inserted into T/A cloning vector to set up the subtractive library. Amplification of the library was carried out with transformation of DH5α. The amplified library contained more than 400 positive bacterial clones, which were then hybridized with forward and backward subtracted probes for differential screening. One hundred positive bacterial clones were randomly selected for sequencing and BLAST analysis. Finally, virtual Northern Blot confirmed such differential expression. The subtracted cDNA library of differentially expressed genes of HSCs was constructed successfully, the library is efficient and lays foundation for screening and cloning new and specific genes of schistosomiasis.
