We present a Brillouin–Raman random fiber laser(BRRFL)with full-open linear cavity structure to generate broadband Brillouin frequency comb(BFC)with double Brillouin-frequency-shift spacing.The incorporation of a reg...We present a Brillouin–Raman random fiber laser(BRRFL)with full-open linear cavity structure to generate broadband Brillouin frequency comb(BFC)with double Brillouin-frequency-shift spacing.The incorporation of a regeneration portion consisting of an erbium-doped fiber and a single-mode fiber enables the generation of broadband BFC.The dynamics of broadband BFC generation changing with the pump power(EDF and Raman)and Brillouin pump(BP)wavelength are investigated in detail,respectively.Under suitable conditions,the bidirectional BRRFL proposed can produce a flatamplitude BFC with 40.7-nm bandwidth ranging from 1531 nm to 1571.7 nm,and built-in 242-order Brillouin Stokes lines(BSLs)with double Brillouin-frequency-shift spacing.Moreover,the linewidth of single BSL is experimentally measured to be about 2.5 kHz.The broadband bidirectional narrow-linewidth BRRFL has great potential applications in optical communication,optical sensing,spectral measurement,and so on.展开更多
为探究表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)在乐果(dimethoate,DIM)经皮肤慢性暴露致小鼠骨骼肌损伤过程中是否具有保护作用,将6周龄雄性ICR小鼠随机分为对照组(腹腔注射0.2 m L生理盐水、背部涂抹0.1 m L生理盐水...为探究表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)在乐果(dimethoate,DIM)经皮肤慢性暴露致小鼠骨骼肌损伤过程中是否具有保护作用,将6周龄雄性ICR小鼠随机分为对照组(腹腔注射0.2 m L生理盐水、背部涂抹0.1 m L生理盐水)、EGCG组(腹腔注射0.2 m L EGCG、背部涂抹0.1 m L生理盐水)、DIM组(腹腔注射0.2 m L生理盐水、背部涂抹0.1 m L DIM)、DIM+EGCG组(腹腔注射0.2 m L EGCG、背部涂抹0.1 m L DIM),记录小鼠体重变化,检测小鼠骨骼肌MDA含量及CAT、T-SOD、T-AOC的活性,利用全自动生化分析仪检测血清CK和LDH活性,利用透射电镜观察骨骼肌超微结构,利用Western-blot检测Bip、PERK/eIF2α通路相关蛋白及成肌分化相关蛋白表达水平。结果显示,与对照组相比,DIM组第2~3周小鼠体重降低显著(P<0.05);第4~8周小鼠体重降低极显著(P<0.01);MDA含量升高极显著(P<0.01),CAT、T-SOD、T-AOC活性降低极显著(P<0.01);血清CK、LDH活性升高极显著(P<0.01);超微结构损伤;内质网应激标志蛋白Bip、PERK/eIF2α通路磷酸化蛋白表达水平显著升高(P<0.05);成肌分化关键蛋白的表达水平降低极显著(P<0.01)。与DIM组相比,DIM+EGCG组小鼠体重无显著差异,总体呈缓慢上升趋势;MDA含量降低极显著(P<0.01),CAT、T-SOD、T-AOC活性升高显著(P<0.05)或升高极显著(P<0.01);血清CK、LDH活性降低显著(P<0.05)或降低极显著(P<0.01);骨骼肌超微结构损伤有所缓解;Bip及PERK/eIF2α通路磷酸化蛋白表达水平降低显著(P<0.05)或降低极显著(P<0.01);成肌分化关键蛋白的表达水平升高显著(P<0.05)或升高极显著(P<0.01)。结果表明,DIM暴露导致了小鼠骨骼肌损伤,引起内质网应激并激活了下游的PERK/eIF2α通路,使小鼠骨骼肌成肌分化受损;EGCG能缓解DIM所致的氧化损伤,提高抗氧化能力,降低血清CK、LDH的活性,显著抑制内质网应激及下游PERK/eIF2α通路磷酸化水平,促进成肌分化蛋白的表达,改善DIM所致的损伤。展开更多
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 62175116 and 91950105)the 1311 Talent Plan of Nanjing University of Posts and Telecommunications, Chinathe Postgraduate Research & Practice Innovation Program, Jiangsu Province, China (Grant No. SJCX21_0276)
文摘We present a Brillouin–Raman random fiber laser(BRRFL)with full-open linear cavity structure to generate broadband Brillouin frequency comb(BFC)with double Brillouin-frequency-shift spacing.The incorporation of a regeneration portion consisting of an erbium-doped fiber and a single-mode fiber enables the generation of broadband BFC.The dynamics of broadband BFC generation changing with the pump power(EDF and Raman)and Brillouin pump(BP)wavelength are investigated in detail,respectively.Under suitable conditions,the bidirectional BRRFL proposed can produce a flatamplitude BFC with 40.7-nm bandwidth ranging from 1531 nm to 1571.7 nm,and built-in 242-order Brillouin Stokes lines(BSLs)with double Brillouin-frequency-shift spacing.Moreover,the linewidth of single BSL is experimentally measured to be about 2.5 kHz.The broadband bidirectional narrow-linewidth BRRFL has great potential applications in optical communication,optical sensing,spectral measurement,and so on.
文摘为探究表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)在乐果(dimethoate,DIM)经皮肤慢性暴露致小鼠骨骼肌损伤过程中是否具有保护作用,将6周龄雄性ICR小鼠随机分为对照组(腹腔注射0.2 m L生理盐水、背部涂抹0.1 m L生理盐水)、EGCG组(腹腔注射0.2 m L EGCG、背部涂抹0.1 m L生理盐水)、DIM组(腹腔注射0.2 m L生理盐水、背部涂抹0.1 m L DIM)、DIM+EGCG组(腹腔注射0.2 m L EGCG、背部涂抹0.1 m L DIM),记录小鼠体重变化,检测小鼠骨骼肌MDA含量及CAT、T-SOD、T-AOC的活性,利用全自动生化分析仪检测血清CK和LDH活性,利用透射电镜观察骨骼肌超微结构,利用Western-blot检测Bip、PERK/eIF2α通路相关蛋白及成肌分化相关蛋白表达水平。结果显示,与对照组相比,DIM组第2~3周小鼠体重降低显著(P<0.05);第4~8周小鼠体重降低极显著(P<0.01);MDA含量升高极显著(P<0.01),CAT、T-SOD、T-AOC活性降低极显著(P<0.01);血清CK、LDH活性升高极显著(P<0.01);超微结构损伤;内质网应激标志蛋白Bip、PERK/eIF2α通路磷酸化蛋白表达水平显著升高(P<0.05);成肌分化关键蛋白的表达水平降低极显著(P<0.01)。与DIM组相比,DIM+EGCG组小鼠体重无显著差异,总体呈缓慢上升趋势;MDA含量降低极显著(P<0.01),CAT、T-SOD、T-AOC活性升高显著(P<0.05)或升高极显著(P<0.01);血清CK、LDH活性降低显著(P<0.05)或降低极显著(P<0.01);骨骼肌超微结构损伤有所缓解;Bip及PERK/eIF2α通路磷酸化蛋白表达水平降低显著(P<0.05)或降低极显著(P<0.01);成肌分化关键蛋白的表达水平升高显著(P<0.05)或升高极显著(P<0.01)。结果表明,DIM暴露导致了小鼠骨骼肌损伤,引起内质网应激并激活了下游的PERK/eIF2α通路,使小鼠骨骼肌成肌分化受损;EGCG能缓解DIM所致的氧化损伤,提高抗氧化能力,降低血清CK、LDH的活性,显著抑制内质网应激及下游PERK/eIF2α通路磷酸化水平,促进成肌分化蛋白的表达,改善DIM所致的损伤。
