目的比较老年股骨粗隆间骨折行改良髋前外侧入路与常规髋外侧入路的疗效。方法回顾性分析2008年2月-2010年2月收治的61例老年股骨粗隆间骨折患者临床资料,其中34例采用经改良髋关节前外侧弧形切口加动力髋螺钉(dynamic hip screw,DHS)固...目的比较老年股骨粗隆间骨折行改良髋前外侧入路与常规髋外侧入路的疗效。方法回顾性分析2008年2月-2010年2月收治的61例老年股骨粗隆间骨折患者临床资料,其中34例采用经改良髋关节前外侧弧形切口加动力髋螺钉(dynamic hip screw,DHS)固定(改良组),27例采用常规髋外侧切口加DHS固定(常规组)。两组患者性别、年龄、致伤原因、Evans分型、合并症、受伤至入院时间、髋关节Harris评分等一般资料比较,差异均无统计学意义(P>0.05),具有可比性。结果两组患者均顺利完成手术。改良组手术时间、术中出血量、术中透视次数、引流量、住院时间及负重天数均显著少于常规组,差异有统计学意义(P<0.05)。两组患者均获随访,随访时间12～24个月,平均18.7个月。患者骨折均获骨性愈合,改良组和常规组骨折愈合时间分别为(11.64±1.28)周和(12.29±1.12)周,比较差异无统计学意义(t=2.15,P=0.15)。术后3、6、12个月两组Harris评分均显著优于术前(P<0.05),术后各时间点间比较差异均有统计学意义(P<0.05);改良组术后各时间点Harris评分均优于常规组(P<0.05)。两组切口感染、肢体短缩、髋内翻畸形愈合、主钉切出股骨头、内固定松动发生率比较差异无统计学意义(P>0.05),但改良组总并发症发生率优于常规组,差异有统计学意义(P<0.05)。结论采用改良髋前外侧入路手术治疗老年股骨粗隆间骨折,能达到充分暴露,并能减少肌肉损伤,降低手术风险,配合术后早期功能锻炼能尽快恢复髋关节功能。展开更多
To investigate the effects of local injection of different doses of lanthanum chloride （LaCl3） on aseptic inflammation in mice stimulated by wear particles from artificial joints, the particles were prepared by vacu...To investigate the effects of local injection of different doses of lanthanum chloride （LaCl3） on aseptic inflammation in mice stimulated by wear particles from artificial joints, the particles were prepared by vacuum ball mill in vitro and air-pouch models were performed with 45 male BALB/c mice that were randomly divided into blank control group, wear particle group and wear parti- cle ＋ LaCl3 （0.1, 0.9 and 8.1 μmol） group. All animals were sacrificed and tissue specimens were harvested 7 days after treatment. Hematoxylin and eosin （H＆E） staining, enzyme-linked immunosorbent assay （ELISA）, reverse transcription-polymerase chain reac- tion （RT-PCR） and western blot were applied to observe inflammatory reaction and detect the expression of pro-inflammatory cyto- kines （TNF-et, IL-1β） and nuclear factor-κB （NF-κB） in mRNA and protein levels in air-pouch membrances. The results showed that wear particles could stimulate aseptic inflammation in vivo effectively; 0.9 μmol LaCl3 could significantly inhibit wear parti- cle-induced gene and protein expression of pro-inflammatory cytokines and NF-Id3 （P〈0.05）; 0.1 and 8. 1 μmol LaCl3 did not exert an inflammation-inhibiting effect and even caused adverse effects at 8.1 μmol. In conclusion, LaC13 played a protective role against wear particle-induced aseptic inflammation dose-dependently, which was involved in NF-κB related signaling pathways.展开更多
To explore the impact of different concentrations of lanthanum chloride （LaC13） on critical components of wear particle-mediated signaling pathways in inflammation and osteoclastogenesis, RAW264.7 cells were natural...To explore the impact of different concentrations of lanthanum chloride （LaC13） on critical components of wear particle-mediated signaling pathways in inflammation and osteoclastogenesis, RAW264.7 cells were naturally divided into eight groups and analyzed by CCK-8 assay, flow cytometry, ELISA, RT-PCR and western blot after treatments. The results showed that three concentrations of LaCI3 had no influence on viability of RAW264.7 cells and down-regulated receptor activator of nuclear factor rd3 （RANK） instead of macrophage colony-stimulating factor receptor （M-CSFR）. Additionally, 2.5 and 10 pmol/L LaC13 could signifi- cantly inhibit gene and protein levels of pro-inflammatory cytokines （tumor necrosis factor-or and interleukin-113, i.e., TNF-ct and IL-113） and NF-r,B/p65, but 100 pmol/L LaC13 did not exert an obvious inflammation-inhibiting effect, and even induced inflamma- tion. In conclusion, these findings demonstrated that LaC13 was able to suppress wear particle-induced inflammation and activation of NF-rd3 in a certain range of concentrations in vitro and mainly decrease the expression of RANK, but not M-CSFR, all of which were generally recognized to play a pivotal role in osteoclastogenesis.展开更多
文摘目的比较老年股骨粗隆间骨折行改良髋前外侧入路与常规髋外侧入路的疗效。方法回顾性分析2008年2月-2010年2月收治的61例老年股骨粗隆间骨折患者临床资料,其中34例采用经改良髋关节前外侧弧形切口加动力髋螺钉(dynamic hip screw,DHS)固定(改良组),27例采用常规髋外侧切口加DHS固定(常规组)。两组患者性别、年龄、致伤原因、Evans分型、合并症、受伤至入院时间、髋关节Harris评分等一般资料比较,差异均无统计学意义(P>0.05),具有可比性。结果两组患者均顺利完成手术。改良组手术时间、术中出血量、术中透视次数、引流量、住院时间及负重天数均显著少于常规组,差异有统计学意义(P<0.05)。两组患者均获随访,随访时间12～24个月,平均18.7个月。患者骨折均获骨性愈合,改良组和常规组骨折愈合时间分别为(11.64±1.28)周和(12.29±1.12)周,比较差异无统计学意义(t=2.15,P=0.15)。术后3、6、12个月两组Harris评分均显著优于术前(P<0.05),术后各时间点间比较差异均有统计学意义(P<0.05);改良组术后各时间点Harris评分均优于常规组(P<0.05)。两组切口感染、肢体短缩、髋内翻畸形愈合、主钉切出股骨头、内固定松动发生率比较差异无统计学意义(P>0.05),但改良组总并发症发生率优于常规组,差异有统计学意义(P<0.05)。结论采用改良髋前外侧入路手术治疗老年股骨粗隆间骨折,能达到充分暴露,并能减少肌肉损伤,降低手术风险,配合术后早期功能锻炼能尽快恢复髋关节功能。
基金supported by National Natural Science Foundation of China (81160222)the Foundation of Health Department of JiangxiProvince (20121044)
文摘To investigate the effects of local injection of different doses of lanthanum chloride （LaCl3） on aseptic inflammation in mice stimulated by wear particles from artificial joints, the particles were prepared by vacuum ball mill in vitro and air-pouch models were performed with 45 male BALB/c mice that were randomly divided into blank control group, wear particle group and wear parti- cle ＋ LaCl3 （0.1, 0.9 and 8.1 μmol） group. All animals were sacrificed and tissue specimens were harvested 7 days after treatment. Hematoxylin and eosin （H＆E） staining, enzyme-linked immunosorbent assay （ELISA）, reverse transcription-polymerase chain reac- tion （RT-PCR） and western blot were applied to observe inflammatory reaction and detect the expression of pro-inflammatory cyto- kines （TNF-et, IL-1β） and nuclear factor-κB （NF-κB） in mRNA and protein levels in air-pouch membrances. The results showed that wear particles could stimulate aseptic inflammation in vivo effectively; 0.9 μmol LaCl3 could significantly inhibit wear parti- cle-induced gene and protein expression of pro-inflammatory cytokines and NF-Id3 （P〈0.05）; 0.1 and 8. 1 μmol LaCl3 did not exert an inflammation-inhibiting effect and even caused adverse effects at 8.1 μmol. In conclusion, LaC13 played a protective role against wear particle-induced aseptic inflammation dose-dependently, which was involved in NF-κB related signaling pathways.
基金supported by National Natural Science Foundation of China(81160222)the Foundation of Health Department of Jiangxi Province(20121044)
文摘To explore the impact of different concentrations of lanthanum chloride （LaC13） on critical components of wear particle-mediated signaling pathways in inflammation and osteoclastogenesis, RAW264.7 cells were naturally divided into eight groups and analyzed by CCK-8 assay, flow cytometry, ELISA, RT-PCR and western blot after treatments. The results showed that three concentrations of LaCI3 had no influence on viability of RAW264.7 cells and down-regulated receptor activator of nuclear factor rd3 （RANK） instead of macrophage colony-stimulating factor receptor （M-CSFR）. Additionally, 2.5 and 10 pmol/L LaC13 could signifi- cantly inhibit gene and protein levels of pro-inflammatory cytokines （tumor necrosis factor-or and interleukin-113, i.e., TNF-ct and IL-113） and NF-r,B/p65, but 100 pmol/L LaC13 did not exert an obvious inflammation-inhibiting effect, and even induced inflamma- tion. In conclusion, these findings demonstrated that LaC13 was able to suppress wear particle-induced inflammation and activation of NF-rd3 in a certain range of concentrations in vitro and mainly decrease the expression of RANK, but not M-CSFR, all of which were generally recognized to play a pivotal role in osteoclastogenesis.
