目的探讨经尿道前列腺等离子双极电切术(transurethral plasmakinetic resection of prostate,TUPKP)治疗高危良性前列腺增生(BPH)患者的临床疗效。方法采用前瞻性多中心研究设计。在全国20家医院泌尿外科按照纳入排除标准,入组行TUPKP...目的探讨经尿道前列腺等离子双极电切术(transurethral plasmakinetic resection of prostate,TUPKP)治疗高危良性前列腺增生(BPH)患者的临床疗效。方法采用前瞻性多中心研究设计。在全国20家医院泌尿外科按照纳入排除标准,入组行TUPKP治疗的高危BPH患者,分析患者基线、围手术期及术后3个月随访的相关数据,评价疗效和安全性。结果2016年9月至2018年12月共入组229名高危BPH患者。与基线相比,术后3个月随访的国际前列腺症状评分改变量为-17.28[95%CI(-18.02,-16.54)]分、最大尿流率改变量为5.61[95%CI(0.68,10.54)]mL·s^(-1)、残余尿量改变量为-84.50[95%CI(-96.49,-72.51)]mL、生活质量评分改变量为-3.24[95%CI(-3.42,-3.06)]分,差异均具有统计学意义(P<0.05)。术中及术后并发症的发生率低,未发生与手术相关的不良事件。结论TUPKP可以用于治疗高危BPH患者,建议由技术熟练的术者实施手术。展开更多
DNA核酶是通过指数富集配体的系统进化技术(Systematic Evolution of Ligands by Exponential Enrichment,SELEX)筛选出来的具有催化活性的DNA分子。本文重点综述了DNA核酶的分类、筛选及主要应用。根据其催化作用方式,可将DNA核酶分为...DNA核酶是通过指数富集配体的系统进化技术(Systematic Evolution of Ligands by Exponential Enrichment,SELEX)筛选出来的具有催化活性的DNA分子。本文重点综述了DNA核酶的分类、筛选及主要应用。根据其催化作用方式,可将DNA核酶分为切割类、连接类、磷酸化类和其他类,其中切割类和连接类占的比例较大。目前DNA核酶的底物主要还是以核酸居多。DNA核酶的筛选主要是采用生物素-链霉亲和素法和聚丙烯酰胺凝胶电泳法来分离有催化活性的DNA分子。目前DNA核酶在金属离子检测、基因治疗和DNA分子加密系统中都呈现出较好的应用前景。展开更多
Aim To study the relationship between the changes of ecology and environments and the transmission of Schistosomiasis japanica after the construction of the three gorges reservoir.Methods Based on predictive data supp...Aim To study the relationship between the changes of ecology and environments and the transmission of Schistosomiasis japanica after the construction of the three gorges reservoir.Methods Based on predictive data supplied by the Yangtze River Committee on Water Conservancy,of the changes of the water level and sediment in the middle and lower reaches of the Yangtze river after the dam construction, corresponding data were collected and mathematics model was extablished by field pilot studies. In order to understand the effects of changes of water level and sediment on the growth 4 distribution of Oncomelania hupensis which is the intermediate host of Schistosoma japonicum, field surveys and ecologically intimated experiments were done.The prevalence status and factors related to transmission were investigated by methods of epidemiology, geographical medicine and social medicine.Results 1 The Three Gorges Project is an integrated project in a large scale to exploit and improve the environments of the Yangtze River. It will have a profound effect on improving and accelerating China’s social and economic development. 2 Positive impact on control of Schistosomiasis after the construction of The Three Gorges Reservoir:After the dam construction, the decrease in the frequency of flooding in Yangtze River will be occurred which cease the diffusion of snails and decline the chances ofinfectionofhumanandlivestock. Snails habitats will be reduced in the flushed areas along the Yangtze River. Exploitation of beaches and delimination of snails will be benefited from the beach’s uplift in the flushed areas. And the floods that will be ahead of time in autumn will interfere the development of snails.3 Negative impact on control of Schistosomiasis after the construction of The Three Gorges Reservoir:The flushed beaches and migrator’s settlements in certain altitude may become snail habitats.The reservoir areas will become the potential transmission areas of Schistosomiasis due to diffusion of infectious resources and Oncomelania snails. The uprising of the water body level may cause the expending of the snails habitats in some place of Jianghan plain in between spring and summer.The perish of Dongting lake will be put off by the delayed flush, which causes the beach in a situation of growing reed-grass or grass for a long time that benefited to snails’ reproduction. And the chances for human and livestock being infected were increased because of the ahead of time of water regression in Dongting beach in autumn.The distribution of snails will not be affected by the changes of water level in the beaches of the downstream of Yangtze River after the dam construction, but the flushed section in Anhui in recent 80 years are suitable for the snails development.4 The environmental changes due to dam construction will not obviously affect the development of snails and the transmission of Schistosomiasis in the Poyang Lake.5 This report is an elementary study and further researches are needed.Conclusion The changes of ecology and environment after dam construction will have positive and negative impact on the transmission of Schistosomiasis in the Three Gorges area and the middle and lower reaches of the Yangtze River.We should consider the negative influences and more studies are needed in making strategies for controlling the transmission of Schistosomiasis. [展开更多
文摘目的探讨经尿道前列腺等离子双极电切术(transurethral plasmakinetic resection of prostate,TUPKP)治疗高危良性前列腺增生(BPH)患者的临床疗效。方法采用前瞻性多中心研究设计。在全国20家医院泌尿外科按照纳入排除标准,入组行TUPKP治疗的高危BPH患者,分析患者基线、围手术期及术后3个月随访的相关数据,评价疗效和安全性。结果2016年9月至2018年12月共入组229名高危BPH患者。与基线相比,术后3个月随访的国际前列腺症状评分改变量为-17.28[95%CI(-18.02,-16.54)]分、最大尿流率改变量为5.61[95%CI(0.68,10.54)]mL·s^(-1)、残余尿量改变量为-84.50[95%CI(-96.49,-72.51)]mL、生活质量评分改变量为-3.24[95%CI(-3.42,-3.06)]分,差异均具有统计学意义(P<0.05)。术中及术后并发症的发生率低,未发生与手术相关的不良事件。结论TUPKP可以用于治疗高危BPH患者,建议由技术熟练的术者实施手术。
基金financially supported by the Fundamental Research Funds for the Central Universities,China(No.2020CDJDPT001)the Chongqing Natural Science Foundation,China(No.cstc2021jcyj-msxm X0699)。
基金supported by the National Natural Science Foundation of China(Nos.52171125,52071178)The fundamental Research Funds for the Central Universities,China(No.2682020ZT114)+1 种基金the Key R&D Projects in the Field of High and New Technology of Sichuan Province,China(Nos.20ZDYF0490,20ZDYF0236)Open Funding of International Joint Laboratory for Light Alloys(MOE),Chongqing University,China。
基金supported by the National Natural Science Foundation of China(Nos.52171125,52071178)the Open Testing Funding of Large Instruments and Equipment of Southwest Jiaotong University,China(No.2022SRII-003)the Open Funding of International Joint Laboratory for Light Alloys(MOE),Chongqing University,China。
文摘DNA核酶是通过指数富集配体的系统进化技术(Systematic Evolution of Ligands by Exponential Enrichment,SELEX)筛选出来的具有催化活性的DNA分子。本文重点综述了DNA核酶的分类、筛选及主要应用。根据其催化作用方式,可将DNA核酶分为切割类、连接类、磷酸化类和其他类,其中切割类和连接类占的比例较大。目前DNA核酶的底物主要还是以核酸居多。DNA核酶的筛选主要是采用生物素-链霉亲和素法和聚丙烯酰胺凝胶电泳法来分离有催化活性的DNA分子。目前DNA核酶在金属离子检测、基因治疗和DNA分子加密系统中都呈现出较好的应用前景。
文摘Aim To study the relationship between the changes of ecology and environments and the transmission of Schistosomiasis japanica after the construction of the three gorges reservoir.Methods Based on predictive data supplied by the Yangtze River Committee on Water Conservancy,of the changes of the water level and sediment in the middle and lower reaches of the Yangtze river after the dam construction, corresponding data were collected and mathematics model was extablished by field pilot studies. In order to understand the effects of changes of water level and sediment on the growth 4 distribution of Oncomelania hupensis which is the intermediate host of Schistosoma japonicum, field surveys and ecologically intimated experiments were done.The prevalence status and factors related to transmission were investigated by methods of epidemiology, geographical medicine and social medicine.Results 1 The Three Gorges Project is an integrated project in a large scale to exploit and improve the environments of the Yangtze River. It will have a profound effect on improving and accelerating China’s social and economic development. 2 Positive impact on control of Schistosomiasis after the construction of The Three Gorges Reservoir:After the dam construction, the decrease in the frequency of flooding in Yangtze River will be occurred which cease the diffusion of snails and decline the chances ofinfectionofhumanandlivestock. Snails habitats will be reduced in the flushed areas along the Yangtze River. Exploitation of beaches and delimination of snails will be benefited from the beach’s uplift in the flushed areas. And the floods that will be ahead of time in autumn will interfere the development of snails.3 Negative impact on control of Schistosomiasis after the construction of The Three Gorges Reservoir:The flushed beaches and migrator’s settlements in certain altitude may become snail habitats.The reservoir areas will become the potential transmission areas of Schistosomiasis due to diffusion of infectious resources and Oncomelania snails. The uprising of the water body level may cause the expending of the snails habitats in some place of Jianghan plain in between spring and summer.The perish of Dongting lake will be put off by the delayed flush, which causes the beach in a situation of growing reed-grass or grass for a long time that benefited to snails’ reproduction. And the chances for human and livestock being infected were increased because of the ahead of time of water regression in Dongting beach in autumn.The distribution of snails will not be affected by the changes of water level in the beaches of the downstream of Yangtze River after the dam construction, but the flushed section in Anhui in recent 80 years are suitable for the snails development.4 The environmental changes due to dam construction will not obviously affect the development of snails and the transmission of Schistosomiasis in the Poyang Lake.5 This report is an elementary study and further researches are needed.Conclusion The changes of ecology and environment after dam construction will have positive and negative impact on the transmission of Schistosomiasis in the Three Gorges area and the middle and lower reaches of the Yangtze River.We should consider the negative influences and more studies are needed in making strategies for controlling the transmission of Schistosomiasis. [
