In order to investigate the effect of curcumin on proliferation and apoptosis of human pterygium fibroblasts (HPF) in culture and search for a new method to prevent the recurrence after pterygium surgery, HPF was incu...In order to investigate the effect of curcumin on proliferation and apoptosis of human pterygium fibroblasts (HPF) in culture and search for a new method to prevent the recurrence after pterygium surgery, HPF was incubated with 0-160 μmol/L curcumin for 24-96 h. The MTT method was used to assay the biologic activities of curcumin at different time points and different doses. The expression of proliferating cell nuclear antigen (PCNA) in each group was detected by immunohistochemistry. The cell cycle distribution was detected by flow cytometry (FCM). Admini- stration of 20-80 μmol/L curcumin for 24-72 h could significantly inhibit HPF proliferation in a dose- and time-dependent manner (P<0.05). After treatment with curcumin at different concentrations of 20, 40, 80 and 160 μmol/L for 24 h, FCM revealed there was a significant sub-G1 peak at each concentration. The number of HPF in G0/G1 phase was increased, while in S phase, it was decreased (P<0.05). At the concentration of 20-80 μmol/L, curcumin, in a dose-dependent manner (P<0.05), could inhibit the expression of PCNA in HPF. It was suggesterd that curcumin could significantly in- hibit the proliferation of HPF, make HPF arrest in G0/G1 phase and induce the apoptosis of HPF in a dose- and time-dependent manner.展开更多
The effects of A771726,the active metabolite of leflunomide,on experimental rat corneal neovascularization(NV) in vivo and on cultured human umbilical vein endothelial cells in vitro were studied.The corneal NV was in...The effects of A771726,the active metabolite of leflunomide,on experimental rat corneal neovascularization(NV) in vivo and on cultured human umbilical vein endothelial cells in vitro were studied.The corneal NV was induced by alkali burn in 40 SD rats.The rats were randomly divided into 4 groups with 10 rats in each group.Group A was treated with 0.9% sodium chloride(control group),and group B,group C and group D were given different concentrations of A771726 eye drops(0.5%,1.0%,2.0% respectively) 4 times daily during days 0-28.The occurrence and development of corneal NV were observed at 4,7,14,21 and 28 day after alkali burn by a slit lamp microscope.The cultured human umbilical vein endothelial cells(ECV-304) were incubated with A771726 solution at different concentrations(20,40,80,160,320 μmol/L) for 36 h.The proliferation of cells was assessed by methyl thiazolyl tetrazolium(MTT),and the expression of proliferating cell nuclear antigen(PCNA) in cells was detected by using immunofluorescence under the laser confocal microscope.The rat model showed that the onset of corneal NV was delayed and progression of corneal NV was inhibited in the groups C and D.The corneal NV areas in groups C and D were significantly smaller than in groups A and B(P<0.01).No significant difference was found in corneal NV areas between groups C and group D(P>0.05).A771726 solution(≥40 μmol/L) could inhibit proliferation of human umbilical vein endothelial cells and decrease the expression of PCNA in cells significantly.A771726,as the active metabolite of leflunomide,strongly prevented corneal NV induced by alkali burn in the in vivo model,and inhibited proliferation of human umbilical vein endothelial cells in the in vitro model.Therefore,A771726 may serve as an angiogenic inhibitor in the treatment of corneal NV.展开更多
文摘In order to investigate the effect of curcumin on proliferation and apoptosis of human pterygium fibroblasts (HPF) in culture and search for a new method to prevent the recurrence after pterygium surgery, HPF was incubated with 0-160 μmol/L curcumin for 24-96 h. The MTT method was used to assay the biologic activities of curcumin at different time points and different doses. The expression of proliferating cell nuclear antigen (PCNA) in each group was detected by immunohistochemistry. The cell cycle distribution was detected by flow cytometry (FCM). Admini- stration of 20-80 μmol/L curcumin for 24-72 h could significantly inhibit HPF proliferation in a dose- and time-dependent manner (P<0.05). After treatment with curcumin at different concentrations of 20, 40, 80 and 160 μmol/L for 24 h, FCM revealed there was a significant sub-G1 peak at each concentration. The number of HPF in G0/G1 phase was increased, while in S phase, it was decreased (P<0.05). At the concentration of 20-80 μmol/L, curcumin, in a dose-dependent manner (P<0.05), could inhibit the expression of PCNA in HPF. It was suggesterd that curcumin could significantly in- hibit the proliferation of HPF, make HPF arrest in G0/G1 phase and induce the apoptosis of HPF in a dose- and time-dependent manner.
文摘The effects of A771726,the active metabolite of leflunomide,on experimental rat corneal neovascularization(NV) in vivo and on cultured human umbilical vein endothelial cells in vitro were studied.The corneal NV was induced by alkali burn in 40 SD rats.The rats were randomly divided into 4 groups with 10 rats in each group.Group A was treated with 0.9% sodium chloride(control group),and group B,group C and group D were given different concentrations of A771726 eye drops(0.5%,1.0%,2.0% respectively) 4 times daily during days 0-28.The occurrence and development of corneal NV were observed at 4,7,14,21 and 28 day after alkali burn by a slit lamp microscope.The cultured human umbilical vein endothelial cells(ECV-304) were incubated with A771726 solution at different concentrations(20,40,80,160,320 μmol/L) for 36 h.The proliferation of cells was assessed by methyl thiazolyl tetrazolium(MTT),and the expression of proliferating cell nuclear antigen(PCNA) in cells was detected by using immunofluorescence under the laser confocal microscope.The rat model showed that the onset of corneal NV was delayed and progression of corneal NV was inhibited in the groups C and D.The corneal NV areas in groups C and D were significantly smaller than in groups A and B(P<0.01).No significant difference was found in corneal NV areas between groups C and group D(P>0.05).A771726 solution(≥40 μmol/L) could inhibit proliferation of human umbilical vein endothelial cells and decrease the expression of PCNA in cells significantly.A771726,as the active metabolite of leflunomide,strongly prevented corneal NV induced by alkali burn in the in vivo model,and inhibited proliferation of human umbilical vein endothelial cells in the in vitro model.Therefore,A771726 may serve as an angiogenic inhibitor in the treatment of corneal NV.
