The paper introduces the research progress in an emergency decision support system for marine pollution （EDSS） in China seas and elaborates on the possible role of the Neutron Activation Analysis （NAA） method ther...The paper introduces the research progress in an emergency decision support system for marine pollution （EDSS） in China seas and elaborates on the possible role of the Neutron Activation Analysis （NAA） method therein. To deal with the increasingly grave situation of offshore pollution, the EDSS for China seas has been researching and developing. Based on the prediction and analysis of the ocean three-dimensional current field, this system makes an inference on the possible path of diffusion and influencing area of marine pollutants and possible location of pollution source, and in combination with the environmentally sensitive information related to the technical integration of GIS, it puts forward the decision, making support for minimizing the hazard caused by pollutants. This system has been operationalized and running for many years on the Bohai and Huanghai seas＇ Marine Pollutants Prediction and Early-Warning, and it has achieved successful experience for many times in the emergencies of China＇s coastal pollution accidents. At present, the environmental guarantee system directed against heavy metals and radioactive pollutants is in the experimental stage. As the NAA method is especially applicable to the detection of part of heavy metals and radioactive substances, it is of very important practical value for the new system to realize the monitoring, prediction and early-warning of ocean heavy metals and radioactive pollutants.展开更多
A method for determination of astaxanthin and astaxanthin eaters in shrimp shell by high performance liquid chromatography is established.Shrimp shell are addressed with 5%hydrochloric acid to remove calcium ions.Afte...A method for determination of astaxanthin and astaxanthin eaters in shrimp shell by high performance liquid chromatography is established.Shrimp shell are addressed with 5%hydrochloric acid to remove calcium ions.After shell is dried,organics from shrimp shell are extracted with anhydrous ethanol.The alcohol extrative of the shrimp shell is mixed with the ammonium sulfate to extract astaxanthin by aqueous two-phase extraction.The crude astaxanthin is collected,which is distributed in the middle layer of the aqueous two-phase layer.After distilled water is added to the crude astaxanthin,the aqueous solution is centrifuged,and the previous step is repeated for several times.The precipitation in centrifuge tube is collected and dried.The crude astaxanthin dried is dissolved with acetone,and the sample solution is separated by TLC.Every pigment on the TLC plate is collected and dissolved with acetone.The pigments are determined by high performance liquid chromatograph.The results show that aqueous two-phase system,3 mL alcohol extractive of astaxanthin and 4.5 mL 20%ammonium sulfate,can be used to acquire crude astaxanthin.The wavelength of the maximum peak of astaxanthin in ethanol solution is 472 nm.A variety of pigments can be separated from the crude astaxanthin by thin-layer chromatography,including free astaxanthin,astaxanthin monoester,astaxanthin diester,echinenone and other substances.It can be seen from high performance liquid chromatography that the appearance time of free astaxanthin is from 4 min to 5.5 min,and the appearance time of astaxanthin monoester is from 10.5 min to 27.8 min.The method is simple about the sample pretreatment and feasible about the determination of astaxanthin and astaxanthin esters in shrimp shell.展开更多
基金supported by the National Natural Science Foundation of China(41206013,41376014,41430963)the Public Science and Technology Research Funds Projects of Ocean (201205018)+5 种基金Key Marine Science Foundation of the State Oceanic Administration of China for Young Scholar(2013203,2012202,2012223)National Sci-Tech Support Plan(2014BAB12B02)Tianjin Sci-Tech Support Plan(14ZCZDSF00012)POL Visiting Fellowship Program (Song Jun)China Scholarship Council ([2008]3019[2012]3013)
文摘The paper introduces the research progress in an emergency decision support system for marine pollution （EDSS） in China seas and elaborates on the possible role of the Neutron Activation Analysis （NAA） method therein. To deal with the increasingly grave situation of offshore pollution, the EDSS for China seas has been researching and developing. Based on the prediction and analysis of the ocean three-dimensional current field, this system makes an inference on the possible path of diffusion and influencing area of marine pollutants and possible location of pollution source, and in combination with the environmentally sensitive information related to the technical integration of GIS, it puts forward the decision, making support for minimizing the hazard caused by pollutants. This system has been operationalized and running for many years on the Bohai and Huanghai seas＇ Marine Pollutants Prediction and Early-Warning, and it has achieved successful experience for many times in the emergencies of China＇s coastal pollution accidents. At present, the environmental guarantee system directed against heavy metals and radioactive pollutants is in the experimental stage. As the NAA method is especially applicable to the detection of part of heavy metals and radioactive substances, it is of very important practical value for the new system to realize the monitoring, prediction and early-warning of ocean heavy metals and radioactive pollutants.
基金supported by Liao Ning Revitalization Talents Program No. XLYC1807161Dalian High-level Talents Innovation Support Plan No. 2017RQ063+4 种基金Dalian Ocean University Zhanlan scholar ProgramThe National Natural Science Foundation of China under contract Nos. 41206013, 41430963the Public Science and Technology Research Funds Projects of Ocean under contract No. 201205018the National Science and Technology Support Program under contract No. 2014BAB12B02Projects of Institute of Marine Industry Technology of Liaoning Universities
文摘A method for determination of astaxanthin and astaxanthin eaters in shrimp shell by high performance liquid chromatography is established.Shrimp shell are addressed with 5%hydrochloric acid to remove calcium ions.After shell is dried,organics from shrimp shell are extracted with anhydrous ethanol.The alcohol extrative of the shrimp shell is mixed with the ammonium sulfate to extract astaxanthin by aqueous two-phase extraction.The crude astaxanthin is collected,which is distributed in the middle layer of the aqueous two-phase layer.After distilled water is added to the crude astaxanthin,the aqueous solution is centrifuged,and the previous step is repeated for several times.The precipitation in centrifuge tube is collected and dried.The crude astaxanthin dried is dissolved with acetone,and the sample solution is separated by TLC.Every pigment on the TLC plate is collected and dissolved with acetone.The pigments are determined by high performance liquid chromatograph.The results show that aqueous two-phase system,3 mL alcohol extractive of astaxanthin and 4.5 mL 20%ammonium sulfate,can be used to acquire crude astaxanthin.The wavelength of the maximum peak of astaxanthin in ethanol solution is 472 nm.A variety of pigments can be separated from the crude astaxanthin by thin-layer chromatography,including free astaxanthin,astaxanthin monoester,astaxanthin diester,echinenone and other substances.It can be seen from high performance liquid chromatography that the appearance time of free astaxanthin is from 4 min to 5.5 min,and the appearance time of astaxanthin monoester is from 10.5 min to 27.8 min.The method is simple about the sample pretreatment and feasible about the determination of astaxanthin and astaxanthin esters in shrimp shell.
