MUC13 is a major gene that controls the susceptibility or resistance to diarrhea caused by ETEC F4 ac in piglets.In this study,the MUC13 resistant allele and performance traits of 3 successive generations of a nucleus...MUC13 is a major gene that controls the susceptibility or resistance to diarrhea caused by ETEC F4 ac in piglets.In this study,the MUC13 resistant allele and performance traits of 3 successive generations of a nucleus Duroc breeding population,a total of 250 pigs,were determined using molecular and conventional breeding techniques to screen out the Duroc population homozygous for MUC13 resistant allele.Under the same feeding and management conditions,the occurrence of diarrhea in same-litter pre-weaned piglets from the 20 litters of breeding sows and 20 litters of propagating sows were 8.7% and 18.4%,respectively（P〈 0.01）.However,there was no significant difference in survival rate of same-litter weaned piglets from breeding sows and propagating sows（P 〈0.05）.The determination results showed there was no significant difference in days of age required by 1stgeneration and 3rd generation to grow to 100 kg of body weight.However,compared to that of the 1st generation,the live backfat thickness of the 3rdgeneration was reduced by 1.46 mm（P 〉0.05）.However,no significant differences in growth rate and live backfat thickness were found among the MUC13 genotypes from all the 3generations（P 0.05）.In conclusion,the homozygosis for the MUC13 resistant allele not only can control the occurrence of diarrhea in piglet but also can reduce significantly live backfat thickness.However,the homozygosis for the MUC13 resistant allele has no significant effects on growth and development of piglets.展开更多
椎体发育同源物(vertebrae development homolog,VRTN)基因g.20311_20312ins291位点和黑素皮质素受体-4(melanocortin-4 receptor,MC4R)基因g.Asp298Asn位点分别是已报道的影响猪脊椎数和脂肪沉积性状的关键突变位点,但上述位点在不同...椎体发育同源物(vertebrae development homolog,VRTN)基因g.20311_20312ins291位点和黑素皮质素受体-4(melanocortin-4 receptor,MC4R)基因g.Asp298Asn位点分别是已报道的影响猪脊椎数和脂肪沉积性状的关键突变位点,但上述位点在不同种群对生长和胴体性状的影响不一,也未见将两位点进行组合研究。因此,本试验采用PCR扩增和PCR-RFLP方法对399头150日龄的美系大白猪群体VRTN基因g.20311_20312ins291位点和MC4R基因g.Asp298Asn位点的多态性进行检测,并分析其与生长和胴体性状的关联性。多态性分析结果表明,VRTN基因g.20311_20312ins291位点在大白猪群体中有Ins/Ins、Ins/Del和Del/Del 3种基因型,基因型频率分别为0.165、0.509和0.326;MC4R基因Asp298Asn位点在大白猪群体中存在GG、AG和AA 3种基因型,基因型频率分别为0.040、0.213、0.747。性状关联分析结果显示,VRTN基因g.20311_20312ins291位点对眼肌面积、瘦肉率有显著影响(P<0.05),对其他性状无显著影响(P>0.05);MC4R基因Asp298Asn位点对体重、日增重、背膘厚、眼肌面积及瘦肉率等性状均有显著影响(P<0.05)。合并基因型对体重、日增重、背膘厚及瘦肉率等4个性状有显著影响(P<0.05),对眼肌面积无显著影响(P>0.05);携带MC4R A等位基因的个体,除Del/Del-AG组合基因型外,无论VRTN是何种基因型,体重和日增重均能表现显著优势(P<0.05),其中Ins/Ins-AA基因型组合最佳。综上可知,VRTN和MC4R基因对大白猪生长和胴体性状具有一定影响,可为后续应用VRTN和MC4R基因辅助选育大白猪提供参考。展开更多
基金Supported by Key Project of Science and Technology Plan of Fujian Province(2010N0012)Agricultural Science and Technology Spanning Plan in Fujian Province(2013KY05)~~
文摘MUC13 is a major gene that controls the susceptibility or resistance to diarrhea caused by ETEC F4 ac in piglets.In this study,the MUC13 resistant allele and performance traits of 3 successive generations of a nucleus Duroc breeding population,a total of 250 pigs,were determined using molecular and conventional breeding techniques to screen out the Duroc population homozygous for MUC13 resistant allele.Under the same feeding and management conditions,the occurrence of diarrhea in same-litter pre-weaned piglets from the 20 litters of breeding sows and 20 litters of propagating sows were 8.7% and 18.4%,respectively（P〈 0.01）.However,there was no significant difference in survival rate of same-litter weaned piglets from breeding sows and propagating sows（P 〈0.05）.The determination results showed there was no significant difference in days of age required by 1stgeneration and 3rd generation to grow to 100 kg of body weight.However,compared to that of the 1st generation,the live backfat thickness of the 3rdgeneration was reduced by 1.46 mm（P 〉0.05）.However,no significant differences in growth rate and live backfat thickness were found among the MUC13 genotypes from all the 3generations（P 0.05）.In conclusion,the homozygosis for the MUC13 resistant allele not only can control the occurrence of diarrhea in piglet but also can reduce significantly live backfat thickness.However,the homozygosis for the MUC13 resistant allele has no significant effects on growth and development of piglets.
