Biothiols such as cysteine(Cys),homocysteine(Hcy)and glutathione(GSH)act as critical roles in maintaining biological redox homeostasis,which is crucial for a plenty of physiological and pathological processes.Therefor...Biothiols such as cysteine(Cys),homocysteine(Hcy)and glutathione(GSH)act as critical roles in maintaining biological redox homeostasis,which is crucial for a plenty of physiological and pathological processes.Therefore,the detection of biothiols is very important and beneficial for many applications.Herein,we have designed and developed a new crystal dimethyl 4-(2,4-dinitrophenylsulfonyloxy)pyridine-2,6-dicarboxylate(P)with 2,4-dinitrobenzene-1-sulfonyl(DNBS)pendant for light-up and detection of biothiols(Cys was selected as the analyte model).The fluorescence"off-on"switch is triggered by the cleavage of DNBS unit by the interaction with biothiols via nucleophilic aromatic substitution reaction.The turn-on fluorescent probe exhibited excellent selectivity and sensitivity toward biothiols.展开更多
基金supported financially by the National Natural Science Foundation of Henan(162300410200)Key Scientific Research Projects of Higher Education of He’nan Province(17A150016)
文摘Biothiols such as cysteine(Cys),homocysteine(Hcy)and glutathione(GSH)act as critical roles in maintaining biological redox homeostasis,which is crucial for a plenty of physiological and pathological processes.Therefore,the detection of biothiols is very important and beneficial for many applications.Herein,we have designed and developed a new crystal dimethyl 4-(2,4-dinitrophenylsulfonyloxy)pyridine-2,6-dicarboxylate(P)with 2,4-dinitrobenzene-1-sulfonyl(DNBS)pendant for light-up and detection of biothiols(Cys was selected as the analyte model).The fluorescence"off-on"switch is triggered by the cleavage of DNBS unit by the interaction with biothiols via nucleophilic aromatic substitution reaction.The turn-on fluorescent probe exhibited excellent selectivity and sensitivity toward biothiols.
