许多细菌在不良环境下能进入活的非可培养状态(viable but nonculturable state,VBNC)。细菌培养技术常常造成VBNC状态的细菌漏检,应用分子生物学检测技术可以提高VBNC细胞的阳性检出率。针对VBNC细胞的分子生物学检测技术,基于细菌特异...许多细菌在不良环境下能进入活的非可培养状态(viable but nonculturable state,VBNC)。细菌培养技术常常造成VBNC状态的细菌漏检,应用分子生物学检测技术可以提高VBNC细胞的阳性检出率。针对VBNC细胞的分子生物学检测技术,基于细菌特异性DNA分子、mRNA分子是目前常用的检测方法,而利用报告基因(如绿色荧光蛋白基因)检测VBNC细胞是一种有效的方法。最近利用叠氮溴乙锭(ethidium monoazide bromide,EMA)或者叠氮溴化丙锭(propidium monoazide,PMA)联合PCR技术选择性抑制细菌死细胞扩增,该方法结合了EMA(PMA)选择渗透性和PCR特异性,作为一种区分细胞死活的方法,可以有效检测细菌VBNC细胞。展开更多
POD activity in heolymph of shrimps determined on 96 microplate was reported first time. In order to testify the sensitivity of using the 96 microwell plate to determine the POD activity, two methods including Worthin...POD activity in heolymph of shrimps determined on 96 microplate was reported first time. In order to testify the sensitivity of using the 96 microwell plate to determine the POD activity, two methods including Worthington and 96 microplate were used to determine the POD of Penaeus japonicus and crawfishes respectively, the results show that the date from the two methods have a strong linear correlation and prove that the method determining POD activity in hemolymph of shrimps was reliable.展开更多
文摘POD activity in heolymph of shrimps determined on 96 microplate was reported first time. In order to testify the sensitivity of using the 96 microwell plate to determine the POD activity, two methods including Worthington and 96 microplate were used to determine the POD of Penaeus japonicus and crawfishes respectively, the results show that the date from the two methods have a strong linear correlation and prove that the method determining POD activity in hemolymph of shrimps was reliable.
