For the purpose of functional validation,the mung bean(Vigna radiata)VrPR gene was cloned and overexpressed in Arabidopsis thaliana.Thefindings revealed that the ORF of VrPR contained 1200 bp,in which 399 amino acids w...For the purpose of functional validation,the mung bean(Vigna radiata)VrPR gene was cloned and overexpressed in Arabidopsis thaliana.Thefindings revealed that the ORF of VrPR contained 1200 bp,in which 399 amino acids were encoded.Bioinformatics analysis showed that the VrPR protein belonged to the NADB Rossmann superfamily,which was one of the non-transmembrane hydrophilic proteins.VrPR was assumed to have 44 amino acid phosphorylation sites and be contained in chloroplasts.The VrPR secondary structure comprised of random coil,αhelix,βangle,and extended chain,all of which were quite compatible with the anticipated tertiary structure.Moreover,analysis of the phylogenetic tree indicated that the soybean PR(Glyma.12G222200)and VrPR were closely related.Furthermore,chlorophyll content in leaves is markedly increased in Arabidopsis when VrPR is overexpressed.Ourfindings will serve as a reference for more functional studies on the PR genes in mung bean.展开更多
Drought is the most important factor limiting plant production in the majority of agricultural crops of the world. Wheat is generally grown on arid-agricultural fields. An experiment was conducted at the Plant physiol...Drought is the most important factor limiting plant production in the majority of agricultural crops of the world. Wheat is generally grown on arid-agricultural fields. An experiment was conducted at the Plant physiology research area, Agronomic Research Institute Faisalabad, during winter 2010-11 to evaluate the effect of drought on wheat variety Lasani 2008. The experiment was comprised of following treatments. T1 Normal moisture (3 IR at CRS, Booting and grain filling), T2 No irrigation (only rainfed) control, T3 water spray (100 ppm), T4 ascorbic acid (100 ppm), T5 salicylic acid (100 ppm), T6 calcium chloride (100 ppm), T7 glycinbetain (100 ppm). According to the resulting data the treatment in which three irrigation were applied produced more no. of tillers (52%), spikelet per spike (41%), spike length (30%), grain per spike (58%), grain yield (54%), biological yield (35%) as compared to control. The treatment in which no irrigation was applied produced less no. of tillers, spikelet per spike, spike length, grain per spike, grain yield, biological yield. Thousand grain weight and harvest index were non significant among all treatments.展开更多
Nitrogen fertilization plays a very important role for crop productivity. New developed wheat varieties need proper fertilization for improved crop productivity. The present study was carried out to quantify, the effe...Nitrogen fertilization plays a very important role for crop productivity. New developed wheat varieties need proper fertilization for improved crop productivity. The present study was carried out to quantify, the effects of nitrogen derived from urea and FYM on the four newly developed wheat varieties i.e. Siran-2009, Ata Habib, Janbaz-2009 and Pirsabak-2008 for yield improvement, quality and soil fertility status. The N treatments were control, 100% of the recommended nitrogen from urea as well as FYM, and 50% from each source. The experiment was carried out at New Developmental Farm, Khyber Pakhtunkhwa Agricultural University Peshawar Pakistan, during Rabi 2011-2012. Results of the data showed that Janbaz-2009 was more responsive to biological yield (11,011 kg·ha-1), grain yield (4339 kg·ha-1), and nitrogen use efficiency (14.8%), whereas Siran-2010 performed better for grain N contents (2.31%). Plots having both urea and FYM had improved biological yield (11,958 kg·ha-1), and grain yield (4901 kg·ha-1). Urea application had improved straw N contents (0.92%) in addition to Mix application of urea and FYM (0.93%). Mix application of both sources and sole FYM had higher grains N content (2.25%), whereas control plots in addition to mix application had improved nitrogen use efficiency (14.8%). Siran-2010 and Janbaz-2009 performed better in FYM and mix FYM and urea plots for most of the parameters. It was concluded from the experiment that Janbaz-2009 had improved yield and yield components, whereas Siran-2010 had improved the grain N content. Similarly, Mix application of FYM and urea had improved crop productivity, soil fertility and grains as well as straw N content. Thus wheat varieties Janbaz-2009 sown in mix FYM and urea is recommended for general cultivation in agro-climatic condition of Peshawar.展开更多
Decision support system for agro-technology transfer (DSSAT), OIL CROP-SUN Model was used to stimulate the phenology, growth, yield of different two sunflower hybrids. i.e. Hysun-33 and S-78 by applying different nitr...Decision support system for agro-technology transfer (DSSAT), OIL CROP-SUN Model was used to stimulate the phenology, growth, yield of different two sunflower hybrids. i.e. Hysun-33 and S-78 by applying different nitrogen levels. The effect of nitrogen (N) on growth and yield components of different sunflower (Helianthus annuus L.) hybrids were evaluated under agro-climatic conditions of Sargodha, Pakistan during spring 2013. The experiment was laid out in a randomized complete block design with split plot arrangement having three replications, keeping cultivars in the main plots and nitrogen levels (0, 45, 90,135 and 180 kg/ha) in sub plots. OIL CROP-SUN Model showed that the model was able to simulate the growth and yield of sunflower with an average of 10.44 error% between observed and simulate achene yield (AY). The result of simulation indicates that nitrogen rate of 180 kg/ha produced highest achene yield in S-78 hybrid as compared to other treatments and Hysun-33 cultivar.展开更多
Polygonatum is a traditional and precious Chinese medicine in China,as well as a traditional medicinal and food homologous material.It has a long history of cultivation and medicinal use.It not only has the effects of...Polygonatum is a traditional and precious Chinese medicine in China,as well as a traditional medicinal and food homologous material.It has a long history of cultivation and medicinal use.It not only has the effects of boosting qi and nourishing yin,nourishing the spleen and lungs,and tonifying the kidney,but also application value in food,health products,ornamental,cosmetics,etc.There are many varieties of Polygonatum resources all over China,which are easy to be mixed.There are three species of medicinal Polygonatum recorded in the 2015 edition of Pharmacopeia of the People s Republic of China.In order to sustainably use and fully develop Polygonatum medicinal resources,we conducted textual research on relevant data and research on the distribution of Polygonatum resources,germplasm identification,genetic diversity and breeding research,so as to provide a reference for the selection and breeding of polygonatum,artificial cultivation and the development and utilization of potential Polygonatum resources and for the solution of the contradiction between supply and demand in the Polygonatum market.展开更多
A field study was carried out with the objective to determine the effect of various levels of nitrogen on growth, development, yield and yield components of different sunflower (Helianthus annuus L.) hybrids i.e. Hysu...A field study was carried out with the objective to determine the effect of various levels of nitrogen on growth, development, yield and yield components of different sunflower (Helianthus annuus L.) hybrids i.e. Hysun-33 and S-78 were evaluated under agro-climatic conditions of Sargodha, Pakistan during spring 2013. The experiment was laid out in a randomized complete block design with split plot arrangement having three replications, keeping cultivars in the main plots and N levels in the subplots. The net plot size was 4.2 m × 6 m. The growth parameters such are leaf area, leaf area duration and yield parameters were observed are Days to a thesis, Days to maturity, head diameter (cm), No. of achene’s per head, Achene yield (kg·ha-1) and total dry matter (kg). The results showed that increasing the nitrogen rate also increased the No. of achene’s per head, Achene yield (kg·ha-1) and total dry matter (kg) in S-78 hybrid where the nitrogen application rate was 180 kg·ha-1 as compared to Hysun-33 cultivar.展开更多
基金This research was funded by National Natural Science Foundation of China,Grant Number(31871576)National Keypoint Research and Invention Program of the Thirteenth,Grant Number(2019YFD1002205)The APC was funded by National Keypoint Research and Invention Program of the Thirteenth.
文摘For the purpose of functional validation,the mung bean(Vigna radiata)VrPR gene was cloned and overexpressed in Arabidopsis thaliana.Thefindings revealed that the ORF of VrPR contained 1200 bp,in which 399 amino acids were encoded.Bioinformatics analysis showed that the VrPR protein belonged to the NADB Rossmann superfamily,which was one of the non-transmembrane hydrophilic proteins.VrPR was assumed to have 44 amino acid phosphorylation sites and be contained in chloroplasts.The VrPR secondary structure comprised of random coil,αhelix,βangle,and extended chain,all of which were quite compatible with the anticipated tertiary structure.Moreover,analysis of the phylogenetic tree indicated that the soybean PR(Glyma.12G222200)and VrPR were closely related.Furthermore,chlorophyll content in leaves is markedly increased in Arabidopsis when VrPR is overexpressed.Ourfindings will serve as a reference for more functional studies on the PR genes in mung bean.
文摘Drought is the most important factor limiting plant production in the majority of agricultural crops of the world. Wheat is generally grown on arid-agricultural fields. An experiment was conducted at the Plant physiology research area, Agronomic Research Institute Faisalabad, during winter 2010-11 to evaluate the effect of drought on wheat variety Lasani 2008. The experiment was comprised of following treatments. T1 Normal moisture (3 IR at CRS, Booting and grain filling), T2 No irrigation (only rainfed) control, T3 water spray (100 ppm), T4 ascorbic acid (100 ppm), T5 salicylic acid (100 ppm), T6 calcium chloride (100 ppm), T7 glycinbetain (100 ppm). According to the resulting data the treatment in which three irrigation were applied produced more no. of tillers (52%), spikelet per spike (41%), spike length (30%), grain per spike (58%), grain yield (54%), biological yield (35%) as compared to control. The treatment in which no irrigation was applied produced less no. of tillers, spikelet per spike, spike length, grain per spike, grain yield, biological yield. Thousand grain weight and harvest index were non significant among all treatments.
文摘Nitrogen fertilization plays a very important role for crop productivity. New developed wheat varieties need proper fertilization for improved crop productivity. The present study was carried out to quantify, the effects of nitrogen derived from urea and FYM on the four newly developed wheat varieties i.e. Siran-2009, Ata Habib, Janbaz-2009 and Pirsabak-2008 for yield improvement, quality and soil fertility status. The N treatments were control, 100% of the recommended nitrogen from urea as well as FYM, and 50% from each source. The experiment was carried out at New Developmental Farm, Khyber Pakhtunkhwa Agricultural University Peshawar Pakistan, during Rabi 2011-2012. Results of the data showed that Janbaz-2009 was more responsive to biological yield (11,011 kg·ha-1), grain yield (4339 kg·ha-1), and nitrogen use efficiency (14.8%), whereas Siran-2010 performed better for grain N contents (2.31%). Plots having both urea and FYM had improved biological yield (11,958 kg·ha-1), and grain yield (4901 kg·ha-1). Urea application had improved straw N contents (0.92%) in addition to Mix application of urea and FYM (0.93%). Mix application of both sources and sole FYM had higher grains N content (2.25%), whereas control plots in addition to mix application had improved nitrogen use efficiency (14.8%). Siran-2010 and Janbaz-2009 performed better in FYM and mix FYM and urea plots for most of the parameters. It was concluded from the experiment that Janbaz-2009 had improved yield and yield components, whereas Siran-2010 had improved the grain N content. Similarly, Mix application of FYM and urea had improved crop productivity, soil fertility and grains as well as straw N content. Thus wheat varieties Janbaz-2009 sown in mix FYM and urea is recommended for general cultivation in agro-climatic condition of Peshawar.
文摘Decision support system for agro-technology transfer (DSSAT), OIL CROP-SUN Model was used to stimulate the phenology, growth, yield of different two sunflower hybrids. i.e. Hysun-33 and S-78 by applying different nitrogen levels. The effect of nitrogen (N) on growth and yield components of different sunflower (Helianthus annuus L.) hybrids were evaluated under agro-climatic conditions of Sargodha, Pakistan during spring 2013. The experiment was laid out in a randomized complete block design with split plot arrangement having three replications, keeping cultivars in the main plots and nitrogen levels (0, 45, 90,135 and 180 kg/ha) in sub plots. OIL CROP-SUN Model showed that the model was able to simulate the growth and yield of sunflower with an average of 10.44 error% between observed and simulate achene yield (AY). The result of simulation indicates that nitrogen rate of 180 kg/ha produced highest achene yield in S-78 hybrid as compared to other treatments and Hysun-33 cultivar.
基金Central Major Expenditure Increase or Decrease Program(2060302)Special Fund for Guiding Local Science and Technology Development by the Central Government(2018SZYD0003)+1 种基金Sichuan Provincial Science and Technology Department Talent Project(2016RZ0061)Sichuan Traditional Chinese Medicine Resources Sustainable Development Strategy Research Project(2016ZR0069).
文摘Polygonatum is a traditional and precious Chinese medicine in China,as well as a traditional medicinal and food homologous material.It has a long history of cultivation and medicinal use.It not only has the effects of boosting qi and nourishing yin,nourishing the spleen and lungs,and tonifying the kidney,but also application value in food,health products,ornamental,cosmetics,etc.There are many varieties of Polygonatum resources all over China,which are easy to be mixed.There are three species of medicinal Polygonatum recorded in the 2015 edition of Pharmacopeia of the People s Republic of China.In order to sustainably use and fully develop Polygonatum medicinal resources,we conducted textual research on relevant data and research on the distribution of Polygonatum resources,germplasm identification,genetic diversity and breeding research,so as to provide a reference for the selection and breeding of polygonatum,artificial cultivation and the development and utilization of potential Polygonatum resources and for the solution of the contradiction between supply and demand in the Polygonatum market.
文摘A field study was carried out with the objective to determine the effect of various levels of nitrogen on growth, development, yield and yield components of different sunflower (Helianthus annuus L.) hybrids i.e. Hysun-33 and S-78 were evaluated under agro-climatic conditions of Sargodha, Pakistan during spring 2013. The experiment was laid out in a randomized complete block design with split plot arrangement having three replications, keeping cultivars in the main plots and N levels in the subplots. The net plot size was 4.2 m × 6 m. The growth parameters such are leaf area, leaf area duration and yield parameters were observed are Days to a thesis, Days to maturity, head diameter (cm), No. of achene’s per head, Achene yield (kg·ha-1) and total dry matter (kg). The results showed that increasing the nitrogen rate also increased the No. of achene’s per head, Achene yield (kg·ha-1) and total dry matter (kg) in S-78 hybrid where the nitrogen application rate was 180 kg·ha-1 as compared to Hysun-33 cultivar.