Microbial infections threaten the health of the Beninese population. Objectives: To identify bioactive phytochemical groups and evaluate the antimicrobial activity of Desmodium ramosissimum used in traditional medicin...Microbial infections threaten the health of the Beninese population. Objectives: To identify bioactive phytochemical groups and evaluate the antimicrobial activity of Desmodium ramosissimum used in traditional medicine in Benin against microbial infections. Methodology & Results: The classic reactions in solution helped highlight alkaloids, anthocyanins, flavonoids, quinones, saponins, steroids, tannins, terpenoids, mucilage, Coumarin, reducing compounds, anthracene, glycosides. The antibacterial activity was evaluated by extracts dilution’s method (ethanol extracts, methanol extracts and hexane extracts). The methanol extract inhibited the growth of all tested germs, with a significant effect on E. coli and S. aureus strains whose MIC values are equal to 1.25 mg/ml. On the contrary, this extract has a low activity on P. vulgaris strain with a MIC of 10 mg/ml. The ethanol extract also inhibited the growth of all the tested strains, but with more intense activity than the methanol extract. The strains of C. albicans and S. oralis were more sensitive to the extract with 0.625 mg/ml for MIC, while S. aureus and P. vulgaris were less sensitive (MIC = 5 mg/ml). The residual ethanol extract exerted inhibition on all microorganisms at a concentration of 20 mg/ml, except the strain of E. foecalis that was resistant. Conclusion & Applications: This study shows that besides its food virtues of this plant, there are antibacterial and antifungal properties.展开更多
Since the time of our ancestors,natural products issued from plant play a therapeutic crucial role.About 25-30%of all medicines(drugs)available for the treatment of diseases are derived from natural products(from plan...Since the time of our ancestors,natural products issued from plant play a therapeutic crucial role.About 25-30%of all medicines(drugs)available for the treatment of diseases are derived from natural products(from plants,animals,bacteria and fungi)or are derivatives of natural products.The aim of this research was to scientifically identify and supply tangible documentation on these two plants employed in the traditional medicine.From November 2020 to February 2021,an ethnobotanical survey was conducted within four markets at the south of Benin,employing a semi-structured questionnaire.Two hundred respondents including 80%of females and 20%of males were interviewed.The 2 studied plants are mainly used for different types of sickness related to infections.Females’herbalists are the most represented.From this research,it appears that the 2 plants are widely used for the treatment of severe infections.On the market,95%of the leafy steam are sold against 5%of the roots for both plants.The main preparation way is decoction.Oral use is reported to be common in all region.The value of samples sold varies from 200F CFA(Financial Cooperation of Africa)to 1000F CFA.The decoctions are usually obtained through one of a mix of different types of plants.Traditional knowledge is transmitted from one generation to another by oral education.Till today there was no record found.During our study,we did no record prohibition or side effect related to these plants’use.These medicinal plants occupy a crucial place within the therapeutic arsenal of west Africa.Our results constitute a vital tool to determine the true potentials of these plants.These results could lead to new improved traditional medicine.展开更多
文摘Microbial infections threaten the health of the Beninese population. Objectives: To identify bioactive phytochemical groups and evaluate the antimicrobial activity of Desmodium ramosissimum used in traditional medicine in Benin against microbial infections. Methodology & Results: The classic reactions in solution helped highlight alkaloids, anthocyanins, flavonoids, quinones, saponins, steroids, tannins, terpenoids, mucilage, Coumarin, reducing compounds, anthracene, glycosides. The antibacterial activity was evaluated by extracts dilution’s method (ethanol extracts, methanol extracts and hexane extracts). The methanol extract inhibited the growth of all tested germs, with a significant effect on E. coli and S. aureus strains whose MIC values are equal to 1.25 mg/ml. On the contrary, this extract has a low activity on P. vulgaris strain with a MIC of 10 mg/ml. The ethanol extract also inhibited the growth of all the tested strains, but with more intense activity than the methanol extract. The strains of C. albicans and S. oralis were more sensitive to the extract with 0.625 mg/ml for MIC, while S. aureus and P. vulgaris were less sensitive (MIC = 5 mg/ml). The residual ethanol extract exerted inhibition on all microorganisms at a concentration of 20 mg/ml, except the strain of E. foecalis that was resistant. Conclusion & Applications: This study shows that besides its food virtues of this plant, there are antibacterial and antifungal properties.
文摘Since the time of our ancestors,natural products issued from plant play a therapeutic crucial role.About 25-30%of all medicines(drugs)available for the treatment of diseases are derived from natural products(from plants,animals,bacteria and fungi)or are derivatives of natural products.The aim of this research was to scientifically identify and supply tangible documentation on these two plants employed in the traditional medicine.From November 2020 to February 2021,an ethnobotanical survey was conducted within four markets at the south of Benin,employing a semi-structured questionnaire.Two hundred respondents including 80%of females and 20%of males were interviewed.The 2 studied plants are mainly used for different types of sickness related to infections.Females’herbalists are the most represented.From this research,it appears that the 2 plants are widely used for the treatment of severe infections.On the market,95%of the leafy steam are sold against 5%of the roots for both plants.The main preparation way is decoction.Oral use is reported to be common in all region.The value of samples sold varies from 200F CFA(Financial Cooperation of Africa)to 1000F CFA.The decoctions are usually obtained through one of a mix of different types of plants.Traditional knowledge is transmitted from one generation to another by oral education.Till today there was no record found.During our study,we did no record prohibition or side effect related to these plants’use.These medicinal plants occupy a crucial place within the therapeutic arsenal of west Africa.Our results constitute a vital tool to determine the true potentials of these plants.These results could lead to new improved traditional medicine.
