BACKGROUND Helicobacter pylori(H.pylori)is a significant human pathogen that is responsible for a variety of illnesses,including mucosa-associated lymphoid tissue lymphoma,gastric cancer,peptic ulcers,and gastritis.AI...BACKGROUND Helicobacter pylori(H.pylori)is a significant human pathogen that is responsible for a variety of illnesses,including mucosa-associated lymphoid tissue lymphoma,gastric cancer,peptic ulcers,and gastritis.AIM To investigate the frequency of H.pylori infection and its resistance patterns among Egyptian patients and to determine the influence of H.pylori virulence genetic determinants on the eradication success of 14-d triple therapy regimen.METHODS H.pylori infections were investigated in 72 patients with gastroduodenal complications suggestive of H.pylori infection.The cagA and vacA genotypes of cultured strains were studied using polymerase chain reaction.The patients underwent 14 d of triple-therapy treatment.The treatment response was examined using histology and a rapid urease test 6 wk after therapy discontinuation.RESULTS The intention-to-treat eradication rate was 59.2%(95%CI:48.2%-70.3%).Rates of H.pylori resistance to clarithromycin,amoxicillin,and metronidazole were 52.8%,81.9%,and 100%,respectively.Successful eradication of H.pylori was more significantly associated with vacA s1-positive strains[adjusted odds ratio(aOR)=0.507,95%CI:0.175-0.822].A significant association was found between failed eradication rate and H.pylori strains resistant to clarithromycin(aOR=0.204,95%CI:-0.005 to 0.412)and amoxicillin(aOR=0.223,95%CI:0.026-0.537).CONCLUSION This study’s low H.pylori eradication rate following 14-d triple therapy is concerning and worrying.H.pylori pan-resistance to metronidazole followed by the high resistance to ciprofloxacin,amoxicillin,and clarithromycin in this research is challenging and of great concern.展开更多
The fungal diversity in harvested apples from organic or conventional management practices was analyzed in different fruit locations(stem end,calyx end,peel,and wounded flesh)shortly after fruit purchase(T1)and after ...The fungal diversity in harvested apples from organic or conventional management practices was analyzed in different fruit locations(stem end,calyx end,peel,and wounded flesh)shortly after fruit purchase(T1)and after 2 weeks of storage(T5).A total of 5,760,162 high-quality fungal sequences were recovered and assigned to 8,504 Operational Taxonomic Units.Members of the phylum Ascomycota were dominant in all samples and accounted for 91.6%of the total number of detected sequences.This was followed by Basidiomycota(8%),Chytridiomycota(0.1%),and unidentified fungi(0.3%).Alpha and beta diversity analyses revealed the presence of significantly different fungal populations in the investigated fruit parts.Among detected fungi,the genus Penicillium prevailed in the peel and in the wounded flesh while Alternaria spp.prevailed in the calyx and stem end samples that included apple core tissues.Several taxonomic units that appear to be closely related to pathogenic fungi associated with secondary human infections were present in peel and wounds.Moreover,significantly different populations were revealed in organic and conventional apples and this result was consistent in all investigated fruit parts(calyx end,peel,stem end,and wounded flesh).Several unique taxa were exclusively detected in organic apples suggesting that management practices may have been a contributing factor in determining the taxa present.In contrast,little differences were revealed in the two assessment times(T1 and T5).Results of the present study represent an advancement of the current knowledge on the fungal microbiota in collected fruit tissues of apple.展开更多
文摘BACKGROUND Helicobacter pylori(H.pylori)is a significant human pathogen that is responsible for a variety of illnesses,including mucosa-associated lymphoid tissue lymphoma,gastric cancer,peptic ulcers,and gastritis.AIM To investigate the frequency of H.pylori infection and its resistance patterns among Egyptian patients and to determine the influence of H.pylori virulence genetic determinants on the eradication success of 14-d triple therapy regimen.METHODS H.pylori infections were investigated in 72 patients with gastroduodenal complications suggestive of H.pylori infection.The cagA and vacA genotypes of cultured strains were studied using polymerase chain reaction.The patients underwent 14 d of triple-therapy treatment.The treatment response was examined using histology and a rapid urease test 6 wk after therapy discontinuation.RESULTS The intention-to-treat eradication rate was 59.2%(95%CI:48.2%-70.3%).Rates of H.pylori resistance to clarithromycin,amoxicillin,and metronidazole were 52.8%,81.9%,and 100%,respectively.Successful eradication of H.pylori was more significantly associated with vacA s1-positive strains[adjusted odds ratio(aOR)=0.507,95%CI:0.175-0.822].A significant association was found between failed eradication rate and H.pylori strains resistant to clarithromycin(aOR=0.204,95%CI:-0.005 to 0.412)and amoxicillin(aOR=0.223,95%CI:0.026-0.537).CONCLUSION This study’s low H.pylori eradication rate following 14-d triple therapy is concerning and worrying.H.pylori pan-resistance to metronidazole followed by the high resistance to ciprofloxacin,amoxicillin,and clarithromycin in this research is challenging and of great concern.
文摘The fungal diversity in harvested apples from organic or conventional management practices was analyzed in different fruit locations(stem end,calyx end,peel,and wounded flesh)shortly after fruit purchase(T1)and after 2 weeks of storage(T5).A total of 5,760,162 high-quality fungal sequences were recovered and assigned to 8,504 Operational Taxonomic Units.Members of the phylum Ascomycota were dominant in all samples and accounted for 91.6%of the total number of detected sequences.This was followed by Basidiomycota(8%),Chytridiomycota(0.1%),and unidentified fungi(0.3%).Alpha and beta diversity analyses revealed the presence of significantly different fungal populations in the investigated fruit parts.Among detected fungi,the genus Penicillium prevailed in the peel and in the wounded flesh while Alternaria spp.prevailed in the calyx and stem end samples that included apple core tissues.Several taxonomic units that appear to be closely related to pathogenic fungi associated with secondary human infections were present in peel and wounds.Moreover,significantly different populations were revealed in organic and conventional apples and this result was consistent in all investigated fruit parts(calyx end,peel,stem end,and wounded flesh).Several unique taxa were exclusively detected in organic apples suggesting that management practices may have been a contributing factor in determining the taxa present.In contrast,little differences were revealed in the two assessment times(T1 and T5).Results of the present study represent an advancement of the current knowledge on the fungal microbiota in collected fruit tissues of apple.
