Inosiplex is a compound formulation composed of inosine and p-acetaminobenzoic acid （PABA） salt of N,N-dimethylamino-2-propanol （DIP）. This study was to investigate the clinical plasma pharmacokiuetic properties o...Inosiplex is a compound formulation composed of inosine and p-acetaminobenzoic acid （PABA） salt of N,N-dimethylamino-2-propanol （DIP）. This study was to investigate the clinical plasma pharmacokiuetic properties of DIP and PABA after single and multiple oral doses of inosiplex tablets in healthy Chinese volunteers. The established LC/MS/MS method for plasma DIP determination had a linear range of 0.02-10 pg/mL, and the HPLC method for plasma PABA determination had a linear range of 0.0540 pg/mL. Linear pharmacokinetic characteristics were found with single oral doses of 0.5, 1.0 and 2.0 g. No obvious accumulation effects were observed for DIP and PABA.展开更多
Simple,rapid and specific liquid chromatography-mass spectrometry（LC-MS） methods have been developed and validated for the quantification of cefcapene acid in human plasma and urine.Plasma samples were simply pretre...Simple,rapid and specific liquid chromatography-mass spectrometry（LC-MS） methods have been developed and validated for the quantification of cefcapene acid in human plasma and urine.Plasma samples were simply pretreated with methanol for deproteinization.Urine samples were briefly diluted with methanol-water（50：50,v/v）,and centrifuged to remove large particles.Chromatographic separation was performed on a Hedera ODS-2 column.For the plasma assay,the isocratic mobile phase consisted of 35% solvent A（Methanol） and 65% solvent B（10 mM ammonium acetate buffer solution containing 0.2% folic acid） with a flow rate of 0.3 mL/min.For the urine assay,the isocratic mobile phase consisted of 30% solvent A（Methanol） and 70% solvent B（10 mM ammonium acetate buffer solution containing 0.2% folic acid） with a flow rate of 0.3 mL/min.The assays were linear over the concentration ranges of 0.03-5 μg/mL in plasma and 0.1-400 μg/mL in urine,and were successfully applied to a pharmacokinetic study after single and multiple oral administrations of cefcapene pivoxil hydrochloride tablets in healthy Chinese volunteers.展开更多
Taking the Qi and Yin deficiency syndrome as an example,the research method of pharmacology of syndrome management system was proposed.By means of text mining,systematic pharmacology and target analysis,to attempt to ...Taking the Qi and Yin deficiency syndrome as an example,the research method of pharmacology of syndrome management system was proposed.By means of text mining,systematic pharmacology and target analysis,to attempt to reveal the essence of the corresponding syndrome by studying the drugs and targets of Qi and Yin deficiency.Fourteen Chinese herbs treating Qi and Yin deficiency were retrieved and used more than 30 times,and 9,317 related targets were predicted.The common targets of action were 85.Topological analysis was carried out by using degree centrality,closeness centrality and betweenness centrality to confirm that estrogen receptor(ESR1),tumor necrosis factor(TNF),D(2)dopamine receptor(DRD2),vitamin D3 receptor(VDR),glucocorticoid receptor(NR3C1),acetylcholinesterase(ACHE)and endothelin-1(EDN1)were highly correlated with Qi and Yin deficiency syndrome.Through the target to find Qi and Yin deficiency syndrome corresponding to 17 categories of diseases.A new idea was provided for studying the biological essence of TCM clinical syndrome differentiation.展开更多
基金financially supported by the Fundamental Research Funds for the Central Universities(Grant no.JKP2011008)the Qing Lan Project,and the Program for New Century ExcellentTalents in University(Grant no.NCET-10-0816)
文摘Inosiplex is a compound formulation composed of inosine and p-acetaminobenzoic acid （PABA） salt of N,N-dimethylamino-2-propanol （DIP）. This study was to investigate the clinical plasma pharmacokiuetic properties of DIP and PABA after single and multiple oral doses of inosiplex tablets in healthy Chinese volunteers. The established LC/MS/MS method for plasma DIP determination had a linear range of 0.02-10 pg/mL, and the HPLC method for plasma PABA determination had a linear range of 0.0540 pg/mL. Linear pharmacokinetic characteristics were found with single oral doses of 0.5, 1.0 and 2.0 g. No obvious accumulation effects were observed for DIP and PABA.
文摘Simple,rapid and specific liquid chromatography-mass spectrometry（LC-MS） methods have been developed and validated for the quantification of cefcapene acid in human plasma and urine.Plasma samples were simply pretreated with methanol for deproteinization.Urine samples were briefly diluted with methanol-water（50：50,v/v）,and centrifuged to remove large particles.Chromatographic separation was performed on a Hedera ODS-2 column.For the plasma assay,the isocratic mobile phase consisted of 35% solvent A（Methanol） and 65% solvent B（10 mM ammonium acetate buffer solution containing 0.2% folic acid） with a flow rate of 0.3 mL/min.For the urine assay,the isocratic mobile phase consisted of 30% solvent A（Methanol） and 70% solvent B（10 mM ammonium acetate buffer solution containing 0.2% folic acid） with a flow rate of 0.3 mL/min.The assays were linear over the concentration ranges of 0.03-5 μg/mL in plasma and 0.1-400 μg/mL in urine,and were successfully applied to a pharmacokinetic study after single and multiple oral administrations of cefcapene pivoxil hydrochloride tablets in healthy Chinese volunteers.
文摘Taking the Qi and Yin deficiency syndrome as an example,the research method of pharmacology of syndrome management system was proposed.By means of text mining,systematic pharmacology and target analysis,to attempt to reveal the essence of the corresponding syndrome by studying the drugs and targets of Qi and Yin deficiency.Fourteen Chinese herbs treating Qi and Yin deficiency were retrieved and used more than 30 times,and 9,317 related targets were predicted.The common targets of action were 85.Topological analysis was carried out by using degree centrality,closeness centrality and betweenness centrality to confirm that estrogen receptor(ESR1),tumor necrosis factor(TNF),D(2)dopamine receptor(DRD2),vitamin D3 receptor(VDR),glucocorticoid receptor(NR3C1),acetylcholinesterase(ACHE)and endothelin-1(EDN1)were highly correlated with Qi and Yin deficiency syndrome.Through the target to find Qi and Yin deficiency syndrome corresponding to 17 categories of diseases.A new idea was provided for studying the biological essence of TCM clinical syndrome differentiation.
