Background: Lower mean platelet volume(MPV) is an indicator of platelet activity in the setting of tumor development. This study was to assess the relationship between preoperative MPV and survival outcomes of patient...Background: Lower mean platelet volume(MPV) is an indicator of platelet activity in the setting of tumor development. This study was to assess the relationship between preoperative MPV and survival outcomes of patients with hepatocellular carcinoma(HCC) following liver transplantation(LT). Methods: The demographic and clinical characteristics of 304 HCC patients following LT were retrieved from an LT database. All the patients were divided into the normal and lower MPV groups according to the median MPV. The factors were first analyzed using a Kaplan–Meier survival analysis, then the factors with P < 0.10 were selected for multivariate Cox regression analysis and were used to define the independent risk factors for poor prognosis. Results: The 1-, 3-, and 5-year tumor free survival was 95.34%, 74.67% and 69.29% in the normal MPV group, respectively, and 95.40%, 59.97% and 42.94% in the lower MPV group, respectively( P < 0.01). No significant difference was observed in post-LT complications between the normal and lower MPV groups. Portal vein tumor thrombosis(PVTT)[hazard ratio(HR = 2.24;95% confidence interval: 1.46–3.43;P < 0.01) and lower MPV(HR = 1.58;95% confidence interval: 1.05–2.36;P = 0.03) were identified as independent prognostic risk factors for recipient survival. Conclusion: Preoperative lower MPV is a risk indicator of HCC patients survival outcomes after LT.展开更多
OBJECTIVE: To investigate the alloimmunogenicity of liver specific antigen and its effects on allolymphocytes. METHODS: Liver specific antigen isolated from inbred F344 rats was used as immunogen to immunize inbred Le...OBJECTIVE: To investigate the alloimmunogenicity of liver specific antigen and its effects on allolymphocytes. METHODS: Liver specific antigen isolated from inbred F344 rats was used as immunogen to immunize inbred Lew rats through different immunization pathways such as low-dose long-term hind footpad, high-dose portal vein and thymus immunization. Western blotting, DNA fragments gel electrophoresis, mixed lymphocyte culture (MLC) and mixed lymphocyte hepatocyte culture (MLHC) were employed to analyze the immune state after immunization. RESULTS: At the time point of sampling, different degree of specific low immunoresponses appeared in all immunized groups as well as cyclophosphamide (CY) treated group. Compared with group I, other groups expressed caspase-3 significantly as detected by using Western blotting. DNA fragment gel electrophoresis of splenocytes showed lymphocyte apoptosis. Compared with the group I, MLC of the experimental groups showed no significant changes except that of the group V, whereas MLHC decreased markedly (P<0.05). CONCLUSIONS: Liver specific antigen not only has alloimmunogenicity to induce alloimmunoreaction but induce antigen specific low immunoresponses and antigen specific lymphocyte apoptosis by high-dose or low-dose long-term immunization. It may be an important transplantation antigen that may lead to a novel way to liver transplantation immunotolerance.展开更多
The cross-section profile is a key signal for evaluating hot-rolled strip quality,and ignoring its defects can easily lead to a final failure.The characteristics of complex curve,significant irregular fluctuation and ...The cross-section profile is a key signal for evaluating hot-rolled strip quality,and ignoring its defects can easily lead to a final failure.The characteristics of complex curve,significant irregular fluctuation and imperfect sample data make it a challenge of recognizing cross-section defects,and current industrial judgment methods rely excessively on human decision making.A novel stacked denoising autoencoders(SDAE)model optimized with support vector machine(SVM)theory was proposed for the recognition of cross-section defects.Firstly,interpolation filtering and principal component analysis were employed to linearly reduce the data dimensionality of the profile curve.Secondly,the deep learning algorithm SDAE was used layer by layer for greedy unsupervised feature learning,and its final layer of back-propagation neural network was replaced by SVM for supervised learning of the final features,and the final model SDAE_SVM was obtained by further optimizing the entire network parameters via error back-propagation.Finally,the curve mirroring and combination stitching methods were used as data augmentation for the training set,which dealt with the problem of sample imbalance in the original data set,and the accuracy of cross-section defect prediction was further improved.The approach was applied in a 1780-mm hot rolling line of a steel mill to achieve the automatic diagnosis and classification of defects in cross-section profile of hot-rolled strip,which helps to reduce flatness quality concerns in downstream processes.展开更多
Objective：The aim of this study was to compare complications and oncologic outcomes of patients undergoing laparoscopic distal pancreatectomy（LDP）and open distal pancreatectomy（ODP）at a single center.Methods：Dis...Objective：The aim of this study was to compare complications and oncologic outcomes of patients undergoing laparoscopic distal pancreatectomy（LDP）and open distal pancreatectomy（ODP）at a single center.Methods：Distal pancreatectomies performed for pancreatic ductal adenocarcinoma during a 4-year period were included in this study.A retrospective analysis of a database of this cohort was conducted.Results：Twenty-two patients underwent LDP for pancreatic ductal adenocarcinoma,in comparison to seventy-six patients with comparable tumor characteristics treated by ODP.No patients with locally advanced lesions were included in this study.Comparing LDP group to ODP group,there were no significant differences in operation time（P=0.06）or blood loss（P=0.24）.Complications（pancreatic fistula,P=0.62;intra-abdominal abscess,P=0.44;postpancreatectomy hemorrhage,P=0.34）were similar.There were no significant differences in the number of lymph nodes harvested（11.2±4.6 in LDP group vs.14.4±5.5 in ODP group,P=0.44）nor the rate of patients with positive lymph nodes（36%in LDP group vs.41%in ODP group,P=0.71）.Incidence of positive margins was similar（9%in LDP group vs.13%in ODP group,P=0.61）.The mean overall survival time was（29.6±3.7）months for the LDP group and（27.6±2.1）months for ODP group.There was no difference in overall survival between the two groups（P=0.34）.Conclusions：LDP is a safe and effective treatment for selected patients with pancreatic ductal adenocarcinoma.A slow-compression of pancreas tissue with the GIA stapler is effective in preventing postoperative pancreatic fistula.The oncologic outcome is comparable with the conventional open approach.Laparoscopic radical antegrade modular pancreatosplenectomy contributed to oncological clearance.展开更多
Acute cellular rejection(ACR) remains a major concern after liver transplantation.Predicting and monitoring acute rejection by non-invasive methods are very important for guiding the use of immunosuppressive drugs.Man...Acute cellular rejection(ACR) remains a major concern after liver transplantation.Predicting and monitoring acute rejection by non-invasive methods are very important for guiding the use of immunosuppressive drugs.Many studies have shown that exosomes and their contents are potential biomarkers for various liver diseases.Here,we identify and validate the role of exosomes and galectin-9 in ACR after liver transplantation.Exosomes were isolated from three sets of paired patients,with and without ACR,and the proteins within the exosomes were isolated and identified.Candidate proteins were then validated using a tissue microarray containing resected liver samples from 73 ACR and 63 non-rejection patients.Finally,protein expression and clinical manifestations were included in KaplanMeier survival and Cox regression analyses.Circulating exosomes were isolated from ACR and non-rejection patients and characterized using transmission electron microscopy and western blotting for CD63/CD81.Western blotting experiments revealed higher levels of galectin-9 protein in circulating exosomes from ACR recipients.Immunohistochemical analysis of the tissue microarray showed that the expression of galectin-9 in resected liver was significantly higher in the ACR group than in the non-rejection group(P<0.05).Higher levels of galectin-9 expression in resected livers were associated with poorer prognosis(P<0.05).Exosome-derived galectin-9 may be a novel predictor of rejection and prognosis after liver transplantation.展开更多
We investigated the prognostic role of regulatory T cells（Tregs） in patients with hepatocellular carcinoma（HCC）. Relevant evidence regarding prognostic significance of Tregs was systematically searched in MEDLINE ...We investigated the prognostic role of regulatory T cells（Tregs） in patients with hepatocellular carcinoma（HCC）. Relevant evidence regarding prognostic significance of Tregs was systematically searched in MEDLINE and Embase databases. A meta-analysis was performed to compare survival in patients with high or low Tregs level（either in peripheral blood or tumor）. Eighteen studies were identified that fulfilled for the eligibility criteria and were included for data synthesis. Our pooled hazard ratios（HRs） demonstrated that increased Tregs intratumoral accumulation was significantly associated with worse overall survival（HR=2.04, 95% confidence interval（CI）： 1.72–2.42） and diseasefree survival（HR=1.82, 95% CI： 1.58–2.09）. Three studies evaluated the role of Tregs in peripheral blood, and all of them showed that increased peripheral Tregs correlated with shortened disease-free and overall survival. Collectively, our results showed that the increased Tregs count is tightly associated with the shortened survivals. Its measurement in either primary tumor or even circulation might be a candidate marker of prognostic significance in HCC patients.展开更多
基金supported by grants from the Natural Science Foundation of Zhejiang Province(Y17H160118,LY18H030002 and LQ15H030003)the Fundamental Research Funds for the Cen-tral University(2018FZA7002)
文摘Background: Lower mean platelet volume(MPV) is an indicator of platelet activity in the setting of tumor development. This study was to assess the relationship between preoperative MPV and survival outcomes of patients with hepatocellular carcinoma(HCC) following liver transplantation(LT). Methods: The demographic and clinical characteristics of 304 HCC patients following LT were retrieved from an LT database. All the patients were divided into the normal and lower MPV groups according to the median MPV. The factors were first analyzed using a Kaplan–Meier survival analysis, then the factors with P < 0.10 were selected for multivariate Cox regression analysis and were used to define the independent risk factors for poor prognosis. Results: The 1-, 3-, and 5-year tumor free survival was 95.34%, 74.67% and 69.29% in the normal MPV group, respectively, and 95.40%, 59.97% and 42.94% in the lower MPV group, respectively( P < 0.01). No significant difference was observed in post-LT complications between the normal and lower MPV groups. Portal vein tumor thrombosis(PVTT)[hazard ratio(HR = 2.24;95% confidence interval: 1.46–3.43;P < 0.01) and lower MPV(HR = 1.58;95% confidence interval: 1.05–2.36;P = 0.03) were identified as independent prognostic risk factors for recipient survival. Conclusion: Preoperative lower MPV is a risk indicator of HCC patients survival outcomes after LT.
文摘OBJECTIVE: To investigate the alloimmunogenicity of liver specific antigen and its effects on allolymphocytes. METHODS: Liver specific antigen isolated from inbred F344 rats was used as immunogen to immunize inbred Lew rats through different immunization pathways such as low-dose long-term hind footpad, high-dose portal vein and thymus immunization. Western blotting, DNA fragments gel electrophoresis, mixed lymphocyte culture (MLC) and mixed lymphocyte hepatocyte culture (MLHC) were employed to analyze the immune state after immunization. RESULTS: At the time point of sampling, different degree of specific low immunoresponses appeared in all immunized groups as well as cyclophosphamide (CY) treated group. Compared with group I, other groups expressed caspase-3 significantly as detected by using Western blotting. DNA fragment gel electrophoresis of splenocytes showed lymphocyte apoptosis. Compared with the group I, MLC of the experimental groups showed no significant changes except that of the group V, whereas MLHC decreased markedly (P<0.05). CONCLUSIONS: Liver specific antigen not only has alloimmunogenicity to induce alloimmunoreaction but induce antigen specific low immunoresponses and antigen specific lymphocyte apoptosis by high-dose or low-dose long-term immunization. It may be an important transplantation antigen that may lead to a novel way to liver transplantation immunotolerance.
基金supported by the National Natural Science Foundation of China(No.52004029)the Joint Doctoral Program of China Scholarship Council(CSC)(202006460073)Liuzhou Science and Technology Plan Project,China(2021AAD0102).
文摘The cross-section profile is a key signal for evaluating hot-rolled strip quality,and ignoring its defects can easily lead to a final failure.The characteristics of complex curve,significant irregular fluctuation and imperfect sample data make it a challenge of recognizing cross-section defects,and current industrial judgment methods rely excessively on human decision making.A novel stacked denoising autoencoders(SDAE)model optimized with support vector machine(SVM)theory was proposed for the recognition of cross-section defects.Firstly,interpolation filtering and principal component analysis were employed to linearly reduce the data dimensionality of the profile curve.Secondly,the deep learning algorithm SDAE was used layer by layer for greedy unsupervised feature learning,and its final layer of back-propagation neural network was replaced by SVM for supervised learning of the final features,and the final model SDAE_SVM was obtained by further optimizing the entire network parameters via error back-propagation.Finally,the curve mirroring and combination stitching methods were used as data augmentation for the training set,which dealt with the problem of sample imbalance in the original data set,and the accuracy of cross-section defect prediction was further improved.The approach was applied in a 1780-mm hot rolling line of a steel mill to achieve the automatic diagnosis and classification of defects in cross-section profile of hot-rolled strip,which helps to reduce flatness quality concerns in downstream processes.
基金supported by the Zhejiang Provincial Natural Science Foundation of China(No.LY17H160026)
文摘Objective：The aim of this study was to compare complications and oncologic outcomes of patients undergoing laparoscopic distal pancreatectomy（LDP）and open distal pancreatectomy（ODP）at a single center.Methods：Distal pancreatectomies performed for pancreatic ductal adenocarcinoma during a 4-year period were included in this study.A retrospective analysis of a database of this cohort was conducted.Results：Twenty-two patients underwent LDP for pancreatic ductal adenocarcinoma,in comparison to seventy-six patients with comparable tumor characteristics treated by ODP.No patients with locally advanced lesions were included in this study.Comparing LDP group to ODP group,there were no significant differences in operation time（P=0.06）or blood loss（P=0.24）.Complications（pancreatic fistula,P=0.62;intra-abdominal abscess,P=0.44;postpancreatectomy hemorrhage,P=0.34）were similar.There were no significant differences in the number of lymph nodes harvested（11.2±4.6 in LDP group vs.14.4±5.5 in ODP group,P=0.44）nor the rate of patients with positive lymph nodes（36%in LDP group vs.41%in ODP group,P=0.71）.Incidence of positive margins was similar（9%in LDP group vs.13%in ODP group,P=0.61）.The mean overall survival time was（29.6±3.7）months for the LDP group and（27.6±2.1）months for ODP group.There was no difference in overall survival between the two groups（P=0.34）.Conclusions：LDP is a safe and effective treatment for selected patients with pancreatic ductal adenocarcinoma.A slow-compression of pancreas tissue with the GIA stapler is effective in preventing postoperative pancreatic fistula.The oncologic outcome is comparable with the conventional open approach.Laparoscopic radical antegrade modular pancreatosplenectomy contributed to oncological clearance.
基金Project supported by the China Postdoctoral Science Foundation(No.2017M610374)the Zhejiang Health Technology Project(No.2019RC153)+1 种基金the Zhejiang Provincial Natural Science Foundation of China(No.Y17H160118)the National Natural Science Foundation of China(No.91542205)
文摘Acute cellular rejection(ACR) remains a major concern after liver transplantation.Predicting and monitoring acute rejection by non-invasive methods are very important for guiding the use of immunosuppressive drugs.Many studies have shown that exosomes and their contents are potential biomarkers for various liver diseases.Here,we identify and validate the role of exosomes and galectin-9 in ACR after liver transplantation.Exosomes were isolated from three sets of paired patients,with and without ACR,and the proteins within the exosomes were isolated and identified.Candidate proteins were then validated using a tissue microarray containing resected liver samples from 73 ACR and 63 non-rejection patients.Finally,protein expression and clinical manifestations were included in KaplanMeier survival and Cox regression analyses.Circulating exosomes were isolated from ACR and non-rejection patients and characterized using transmission electron microscopy and western blotting for CD63/CD81.Western blotting experiments revealed higher levels of galectin-9 protein in circulating exosomes from ACR recipients.Immunohistochemical analysis of the tissue microarray showed that the expression of galectin-9 in resected liver was significantly higher in the ACR group than in the non-rejection group(P<0.05).Higher levels of galectin-9 expression in resected livers were associated with poorer prognosis(P<0.05).Exosome-derived galectin-9 may be a novel predictor of rejection and prognosis after liver transplantation.
基金supported by the National Natural Science Foundation of China(No.81370644)the Zhejiang Provincial Natural Science Foundation of China(No.LY17H60026)
文摘We investigated the prognostic role of regulatory T cells（Tregs） in patients with hepatocellular carcinoma（HCC）. Relevant evidence regarding prognostic significance of Tregs was systematically searched in MEDLINE and Embase databases. A meta-analysis was performed to compare survival in patients with high or low Tregs level（either in peripheral blood or tumor）. Eighteen studies were identified that fulfilled for the eligibility criteria and were included for data synthesis. Our pooled hazard ratios（HRs） demonstrated that increased Tregs intratumoral accumulation was significantly associated with worse overall survival（HR=2.04, 95% confidence interval（CI）： 1.72–2.42） and diseasefree survival（HR=1.82, 95% CI： 1.58–2.09）. Three studies evaluated the role of Tregs in peripheral blood, and all of them showed that increased peripheral Tregs correlated with shortened disease-free and overall survival. Collectively, our results showed that the increased Tregs count is tightly associated with the shortened survivals. Its measurement in either primary tumor or even circulation might be a candidate marker of prognostic significance in HCC patients.
