Tracking moving particles in cells by single particle tracking is an important optical approach widely used in biological research. In order to track multiple particles within a whole cell simultaneously, a parallel t...Tracking moving particles in cells by single particle tracking is an important optical approach widely used in biological research. In order to track multiple particles within a whole cell simultaneously, a parallel tracking approach with large depth of field was put forward. It was based on distorted grating and dual-objective bifocal imaging, making use of the distorted grating to expand the depth of field, dual-objective to gather as many photons as possible, and bifocal plane imaging to realize three-dimensional localization. Simulation of parallel tracking of two particles moving along the z axis demonstrated that even when the two are axially separated by10 μm, they can both be localized simultaneously with transversal precision better than 5 nm and axial precision better than 20 nm.展开更多
基金supported by the National Natural Science Foundation of China(Nos.11774242,61975131,61605120,and 61335001)Specially Funded Program on National Key Scientific Instruments and Equipment Development(No.2012YQ15009203)+1 种基金Natural Science Foundation of Guangdong Province(No.2018A030313362)Shenzhen Science and Technology Planning Project(Nos.JCYJ20170818142804605 and JCYJ20170818141701667)
文摘Tracking moving particles in cells by single particle tracking is an important optical approach widely used in biological research. In order to track multiple particles within a whole cell simultaneously, a parallel tracking approach with large depth of field was put forward. It was based on distorted grating and dual-objective bifocal imaging, making use of the distorted grating to expand the depth of field, dual-objective to gather as many photons as possible, and bifocal plane imaging to realize three-dimensional localization. Simulation of parallel tracking of two particles moving along the z axis demonstrated that even when the two are axially separated by10 μm, they can both be localized simultaneously with transversal precision better than 5 nm and axial precision better than 20 nm.
