目的生长动态的监测是肿瘤学重要的研究内容之一,现有的各种生长动态的监测方法存在诸多缺陷。文中研究活体成像技术(in vivo imaging technique,IVIT)在肿瘤生长动态监测中的应用。方法在18只Babl/C小鼠背部皮下注射1×106/mL B16-...目的生长动态的监测是肿瘤学重要的研究内容之一,现有的各种生长动态的监测方法存在诸多缺陷。文中研究活体成像技术(in vivo imaging technique,IVIT)在肿瘤生长动态监测中的应用。方法在18只Babl/C小鼠背部皮下注射1×106/mL B16-F10-luc-G5细胞100μL,建立种植瘤模型。肿块出现后每天测量肿瘤并计算肿瘤体积,并于第0、3、5、7、9、14天每次取3只小鼠以活体成像系统(in vivo imaging system,IVIS)检测肿瘤生物发光,检测后处死取肿瘤组织制成石蜡切片行病理学检查。结果所有小鼠接种成功肿瘤,第5天肿瘤可见。第5、7、9、14天肿瘤大小分别为[(2.86E+00)±1.21]、[(4.87E+00)±1.66]、[(9.27E+01)±6.31]、[(2.60E+02)±7.88]mm3;所有移植瘤生物发光均被IVIS检测到,第0、3、5、7、9、14天肿瘤平均实测光子数为[(6.35E+05)±7655]、[(1.12E+05)±1820]、[(1.62E+05)±2090]、[(2.40E+05)±3515]、[(1.18E+06)±11530]、[(2.23E+06)±17934]photon/(cm2·ser·s);移植瘤平均实测光子数与体积之间存在线性回归关系(R2=0.97);以IVIS监测结果绘制的肿瘤生长曲线,揭示了B16F10移植瘤生长动态特点。结论 IVIT可连续监测标记肿瘤的生长动态,准确灵敏、具有一定实用价值。展开更多
Juvenile xanthogranuloma (JXG) is an uncommon benign disease, which usually occurs on the skin of head and neck region, as a single or multiple nodular lesions. Tracheal involvement of JXG is extremely rare, and onl...Juvenile xanthogranuloma (JXG) is an uncommon benign disease, which usually occurs on the skin of head and neck region, as a single or multiple nodular lesions. Tracheal involvement of JXG is extremely rare, and only few cases have been reported in literature. Herein we presented an additional new case and discussed the optimal management of this uncommon disease.展开更多
文摘目的生长动态的监测是肿瘤学重要的研究内容之一,现有的各种生长动态的监测方法存在诸多缺陷。文中研究活体成像技术(in vivo imaging technique,IVIT)在肿瘤生长动态监测中的应用。方法在18只Babl/C小鼠背部皮下注射1×106/mL B16-F10-luc-G5细胞100μL,建立种植瘤模型。肿块出现后每天测量肿瘤并计算肿瘤体积,并于第0、3、5、7、9、14天每次取3只小鼠以活体成像系统(in vivo imaging system,IVIS)检测肿瘤生物发光,检测后处死取肿瘤组织制成石蜡切片行病理学检查。结果所有小鼠接种成功肿瘤,第5天肿瘤可见。第5、7、9、14天肿瘤大小分别为[(2.86E+00)±1.21]、[(4.87E+00)±1.66]、[(9.27E+01)±6.31]、[(2.60E+02)±7.88]mm3;所有移植瘤生物发光均被IVIS检测到,第0、3、5、7、9、14天肿瘤平均实测光子数为[(6.35E+05)±7655]、[(1.12E+05)±1820]、[(1.62E+05)±2090]、[(2.40E+05)±3515]、[(1.18E+06)±11530]、[(2.23E+06)±17934]photon/(cm2·ser·s);移植瘤平均实测光子数与体积之间存在线性回归关系(R2=0.97);以IVIS监测结果绘制的肿瘤生长曲线,揭示了B16F10移植瘤生长动态特点。结论 IVIT可连续监测标记肿瘤的生长动态,准确灵敏、具有一定实用价值。
文摘Juvenile xanthogranuloma (JXG) is an uncommon benign disease, which usually occurs on the skin of head and neck region, as a single or multiple nodular lesions. Tracheal involvement of JXG is extremely rare, and only few cases have been reported in literature. Herein we presented an additional new case and discussed the optimal management of this uncommon disease.