Diversity analysis among 23 rice varieties including 16 non-basmati scented accessions,5 basmati accessions and 2 non-scented accessions was performed by random amplified polymorphic DNA(RAPD) and inter-simple sequenc...Diversity analysis among 23 rice varieties including 16 non-basmati scented accessions,5 basmati accessions and 2 non-scented accessions was performed by random amplified polymorphic DNA(RAPD) and inter-simple sequence repeat(ISSR) marker systems.The varieties analyzed by 11 RAPD and 8 ISSR primers yielded an average of 65% and 80% polymorphism,respectively.The average number of polymorphic bands generated per RAPD primer was 6 and per ISSR primer was 5.87.RAPD and ISSR data analysis individually could not segregate basmati and non-basmati scented rice accessions.However,the analysis using a combined data could group basmati and non-basmati scented rice accessions separately.The bands present specifically among three accessions of non-basmati scented rice were also identified.The study revealed a high genetic diversity among non-basmati scented rice accessions.展开更多
文摘Diversity analysis among 23 rice varieties including 16 non-basmati scented accessions,5 basmati accessions and 2 non-scented accessions was performed by random amplified polymorphic DNA(RAPD) and inter-simple sequence repeat(ISSR) marker systems.The varieties analyzed by 11 RAPD and 8 ISSR primers yielded an average of 65% and 80% polymorphism,respectively.The average number of polymorphic bands generated per RAPD primer was 6 and per ISSR primer was 5.87.RAPD and ISSR data analysis individually could not segregate basmati and non-basmati scented rice accessions.However,the analysis using a combined data could group basmati and non-basmati scented rice accessions separately.The bands present specifically among three accessions of non-basmati scented rice were also identified.The study revealed a high genetic diversity among non-basmati scented rice accessions.
