Qichang Dong was a famous calligraphy and painting artist in ancient China. The theory of southern and northern schools proposed by him was a conclusion to traditional Chinese landscape painting art, and played an inc...Qichang Dong was a famous calligraphy and painting artist in ancient China. The theory of southern and northern schools proposed by him was a conclusion to traditional Chinese landscape painting art, and played an incomparable function in the traditional Chinese painting history in comparison with other painting theories. In the painting history, there were the theory of ＂pre-Dong＂ and the theory of ＂post-Dong＂ . In this paper, by starting from Qichang Dong＇ s ＂theory of southern and northern schoots＂ , the homogeneities between traditional Chinese music and painting arts are analyzed from the principle of spirit and the principle of technology, so that the homogeneities between traditional Chinese music and painting arts are explored in aesthetic concept and actual art creation.展开更多
The idea of ＂worship elegance＂ in Zhu Xi＇ are ＂worship elegance＂ and ＂express aspiration＂ , namely thoughts s music thoughts has been commonly discussed by most theorists, but there ＂restrain feelings＂ and ＂...The idea of ＂worship elegance＂ in Zhu Xi＇ are ＂worship elegance＂ and ＂express aspiration＂ , namely thoughts s music thoughts has been commonly discussed by most theorists, but there ＂restrain feelings＂ and ＂express feelings＂ co-existing in Zhu xi＇ s music thoughts.展开更多
The 2019 novel coronavirus disease(COVID-19)outbreak is a global crisis that has placed small and medium enterprises(SMEs)under huge pressure to survive,requiring them to respond effectively to the crisis.SMEs have ad...The 2019 novel coronavirus disease(COVID-19)outbreak is a global crisis that has placed small and medium enterprises(SMEs)under huge pressure to survive,requiring them to respond effectively to the crisis.SMEs have adopted various digital tech no logies to cope with this crisis.Using a data set from a survey with 518 Chin ese SMEs,the study examines the relati on ship between SMEs'digitalizatio n and their public crisis responses.The empirical results show that digitalization has enabled SMEs to respond effectively to the public crisis by making use of their dynamic capabilities.In addition,digitalization can help improve SMEs'performance.We propose a theoretical framework ofdigitalization and crisis respohses for SMEs and present three avenues for future research.展开更多
Background:Apparent Young’s modulus(AYM),which reflects the fundamental mechanical property of live cells measured by atomic force microscopy and is determined by substrate stiffness regulated cytoskeletal organizati...Background:Apparent Young’s modulus(AYM),which reflects the fundamental mechanical property of live cells measured by atomic force microscopy and is determined by substrate stiffness regulated cytoskeletal organization,has been investigated as potential indicators of cell fate in specific cell types.However,applying biophysical cues,such as modulating the substrate stiffness,to regulate AYM and thereby reflect and/or control stem cell lineage specificity for downstream applications,remains a primary challenge during in vitro stem cell expansion.Moreover,substrate stiffness could modulate cell heterogeneity in the single-cell stage and contribute to cell fate regulation,yet the indicative link between AYM and cell fate determination during in vitro dynamic cell expansion(from single-cell stage to multi-cell stage)has not been established.Results:Here,we show that the AYM of cells changed dynamically during passaging and proliferation on substrates with different stiffness.Moreover,the same change in substrate stiffness caused different patterns of AYM change in epithelial and mesenchymal cell types.Embryonic stem cells and their derived progenitor cells exhibited distinguishing AYM changes in response to different substrate stiffness that had significant effects on their maintenance of pluripotency and/or lineage-specific characteristics.On substrates that were too rigid or too soft,fluctuations in AYM occurred during cell passaging and proliferation that led to a loss in lineage specificity.On a substrate with‘optimal’stiffness(i.e.,3.5 kPa),the AYM was maintained at a constant level that was consistent with the parental cells during passaging and proliferation and led to preservation of lineage specificity.The effects of substrate stiffness on AYM and downstream cell fate were correlated with intracellular cytoskeletal organization and nuclear/cytoplasmic localization of YAP.Conclusions:In summary,this study suggests that optimal substrate stiffness regulated consistent AYM during passaging and proliferation reflects and contributes to hESCs and their derived progenitor cells lineage specificity maintenance,through the underlying mechanistic pathways of stiffness-induced cytoskeletal organization and the downstream YAP signaling.These findings highlighted the potential of AYM as an indicator to select suitable substrate stiffness for stem cell specificity maintenance during in vitro expansion for regenerative applications.展开更多
文摘Qichang Dong was a famous calligraphy and painting artist in ancient China. The theory of southern and northern schools proposed by him was a conclusion to traditional Chinese landscape painting art, and played an incomparable function in the traditional Chinese painting history in comparison with other painting theories. In the painting history, there were the theory of ＂pre-Dong＂ and the theory of ＂post-Dong＂ . In this paper, by starting from Qichang Dong＇ s ＂theory of southern and northern schoots＂ , the homogeneities between traditional Chinese music and painting arts are analyzed from the principle of spirit and the principle of technology, so that the homogeneities between traditional Chinese music and painting arts are explored in aesthetic concept and actual art creation.
文摘The idea of ＂worship elegance＂ in Zhu Xi＇ are ＂worship elegance＂ and ＂express aspiration＂ , namely thoughts s music thoughts has been commonly discussed by most theorists, but there ＂restrain feelings＂ and ＂express feelings＂ co-existing in Zhu xi＇ s music thoughts.
基金supported by the National Natural Science Foundation of China under Grant 71872178。
文摘The 2019 novel coronavirus disease(COVID-19)outbreak is a global crisis that has placed small and medium enterprises(SMEs)under huge pressure to survive,requiring them to respond effectively to the crisis.SMEs have adopted various digital tech no logies to cope with this crisis.Using a data set from a survey with 518 Chin ese SMEs,the study examines the relati on ship between SMEs'digitalizatio n and their public crisis responses.The empirical results show that digitalization has enabled SMEs to respond effectively to the public crisis by making use of their dynamic capabilities.In addition,digitalization can help improve SMEs'performance.We propose a theoretical framework ofdigitalization and crisis respohses for SMEs and present three avenues for future research.
基金This work was financially supported by the Beijing Municipal Science&Technology Commission(Z181100001818005)the National Natural Science Foundation of China(31671036)and Beijing Natural Science Foundation(JQ18022)the Australian National Health and Medical Research Council(APP1120249).
文摘Background:Apparent Young’s modulus(AYM),which reflects the fundamental mechanical property of live cells measured by atomic force microscopy and is determined by substrate stiffness regulated cytoskeletal organization,has been investigated as potential indicators of cell fate in specific cell types.However,applying biophysical cues,such as modulating the substrate stiffness,to regulate AYM and thereby reflect and/or control stem cell lineage specificity for downstream applications,remains a primary challenge during in vitro stem cell expansion.Moreover,substrate stiffness could modulate cell heterogeneity in the single-cell stage and contribute to cell fate regulation,yet the indicative link between AYM and cell fate determination during in vitro dynamic cell expansion(from single-cell stage to multi-cell stage)has not been established.Results:Here,we show that the AYM of cells changed dynamically during passaging and proliferation on substrates with different stiffness.Moreover,the same change in substrate stiffness caused different patterns of AYM change in epithelial and mesenchymal cell types.Embryonic stem cells and their derived progenitor cells exhibited distinguishing AYM changes in response to different substrate stiffness that had significant effects on their maintenance of pluripotency and/or lineage-specific characteristics.On substrates that were too rigid or too soft,fluctuations in AYM occurred during cell passaging and proliferation that led to a loss in lineage specificity.On a substrate with‘optimal’stiffness(i.e.,3.5 kPa),the AYM was maintained at a constant level that was consistent with the parental cells during passaging and proliferation and led to preservation of lineage specificity.The effects of substrate stiffness on AYM and downstream cell fate were correlated with intracellular cytoskeletal organization and nuclear/cytoplasmic localization of YAP.Conclusions:In summary,this study suggests that optimal substrate stiffness regulated consistent AYM during passaging and proliferation reflects and contributes to hESCs and their derived progenitor cells lineage specificity maintenance,through the underlying mechanistic pathways of stiffness-induced cytoskeletal organization and the downstream YAP signaling.These findings highlighted the potential of AYM as an indicator to select suitable substrate stiffness for stem cell specificity maintenance during in vitro expansion for regenerative applications.
