Glycogen plays essential roles in glucose metabolism.Imaging glycogen in the liver,the major glycogen reservoir in the body,may shed new light on many metabolic disorders.^(13)C magnetic resonance spectroscopy(MRS)has...Glycogen plays essential roles in glucose metabolism.Imaging glycogen in the liver,the major glycogen reservoir in the body,may shed new light on many metabolic disorders.^(13)C magnetic resonance spectroscopy(MRS)has become the mainstream method for monitoring glycogen in the body.However,the equipment of special hard-ware to standard clinical magnetic resonance imaging(MRI)scanners limits its clinical applications.Herein,we utilized endogenous glycogen as a T_(2)-based relaxation contrast agent for imaging glycogen metabolism in the liver in vivo.The in vitro results demonstrated that the transverse relaxation rate of glycogen strongly correlates with the concentration,pH,and field strength.Based on the Swift-Connick theory,we characterized the exchange property of glycogen and measured the exchange rate of glycogen as 31,847 Hz at 37°C.Besides,the viscosity and echo spacing showed no apparent effect on the transverse relaxation rate.This unique feature enables vi-sualization of glycogen signaling in vivo through T_(2)-weighted MRI.Two hours-post intraperitoneal injection of glucagon,a clinical drug to promote glycogenolysis and gluconeogenesis,the signal intensity of the mice’s liver increased by 1.8 times from the T_(2)-weighted imaging experiment due to the decomposition of glycogen.This study provides a convenient imaging strategy to non-invasively investigate glycogen metabolism in the liver,which may find clinical applications in metabolic diseases.展开更多
Mammalian mitochondria have small genomes encoding very limited numbers of proteins.Over one thousand proteins and noncoding RNAs encoded by the nuclear genome must be imported from the cytosol into the mitochondria.H...Mammalian mitochondria have small genomes encoding very limited numbers of proteins.Over one thousand proteins and noncoding RNAs encoded by the nuclear genome must be imported from the cytosol into the mitochondria.Here,we report the identification of hundreds of circular RNAs(mecciRNAs)encoded by the mitochondrial genome.We provide both in vitro and in vivo evidence to show that mecciRNAs facilitate the mitochondrial entry of nuclear-encoded proteins by serving as molecular chaperones in the folding of imported proteins.Known components involved in mitochondrial protein and RNA importation,such as TOM40 and PNPASE,interact with mecciRNAs and regulate protein entry.The expression of mecciRNAs is regulated,and these transcripts are critical for the adaption of mitochondria to physiological conditions and diseases such as stresses and cancers by modulating mitochondrial protein importation.mecciRNAs and their associated physiological roles add categories and functions to the known eukaryotic circular RNAs and shed novel light on the communication between mitochondria and the nucleus.展开更多
Mechanized rice direct seeding is a cost-effective and efficient approach for rice cultivation.Recently,the use of rice direct seeding has been increasing rapidly owing to rural labour shortages and continuous increas...Mechanized rice direct seeding is a cost-effective and efficient approach for rice cultivation.Recently,the use of rice direct seeding has been increasing rapidly owing to rural labour shortages and continuous increases in agricultural production costs.This article reviews the research and application progress of mechanized rice direct seeding including direct seeding technologies,precision rice seeding,precision rice seed-metering devices,key supporting agronomy technologies for mechanized rice direct seeding.South China Agricultural University developed precision rice hill-drop drilling(PRHDD)with synchronous furrowing and ridging technology and series machines for paddy that affords remarkable advantages in terms of saving time and labour,higher yield,and higher efficiency.In this approach,pre-germinated seeds are uniformly hill-dropped in the expected positions in puddled soil.It significantly improved the crop growth population and effectively solved the problems of high frequency of disease and pests caused by the irregular distribution of rice seeds with manual broadcasting,and generally reduces seed usage and increases the yield.Therefore,this technology has broad application prospects and great potential for promoting the development of mechanized rice direct seeding in China.展开更多
To meet the requirement of the precision direct-seeding for hybrid rice,this study aimed to design a hill-drop pneumatic central cylinder direct-seeding machine to sow ten rows at a time.A series of orthogonal experim...To meet the requirement of the precision direct-seeding for hybrid rice,this study aimed to design a hill-drop pneumatic central cylinder direct-seeding machine to sow ten rows at a time.A series of orthogonal experiments were conducted to investigate the performance of the cylinder seeder.The influences of the hole diameters,degree of vacuum,and rotational speed of the cylinder were tested on JPS-12 computer-vision seeding test platform,and the rotational speed of 10-50 r/min,diameter of 135 mm and a negative pressure of 1.0-2.0 kPa were employed.Test results showed that the optimal parameter combination was a vacuum of 2.0 kPa and a hole diameter of 2.0 mm(straight hole),with a rotational speed of 30 r/min.The probability of(2±1)seeds in each hole was 95.3%,while the probability of seed-missing hole was 2.0%.A series of field experiments were then conducted to test the seeder performance according to China National Standard Test Methods,and the field test results showed that for the hill-drop pneumatic central cylinder direct-seeding machine,the probability of(2±1)seeds in each hill was 91.6%,while the probability of seed-missing hill was 2.7%.The yield data showed that the average effective panicle had 231.25 ears,the average seed setting rate was 9.92%,the average 1000-grain weight was 22.45 g,and the average yield was 7107.9 kg/hm^(2) that was 26.14%higher than the average yield of rice(5634.9 kg/hm^(2))of Guangdong Province in 2016.The results showed that the hill-drop pneumatic central cylinder direct-seeding machine for hybrid rice could be applied in practical precision rice seeding.展开更多
Dear Editor,The spread of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)led to a global pandemic with 260 million infected people.A variety of vaccines,including the mRNA and recombinant vaccines,were dev...Dear Editor,The spread of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)led to a global pandemic with 260 million infected people.A variety of vaccines,including the mRNA and recombinant vaccines,were developed to prevent the spread of the disease.1,2 There is an urgent need for testing the vaccine-induced long-term memory response based on the immunological principles and identifying the optimal combination of dose and injection manner to provide important information for clinical application.展开更多
基金This work is supported by the National Key R&D Program of China(2018YFA0704000)the National Natural Science Foundation of China(91859206,U21A20392,82127802 and 21921004)+2 种基金the Key Research Program of Frontier Sciences,Chinese Academy of Sciences(ZDBS-LY-JSC004)the Scientific Instrument Developing Project of the Chi-nese Academy of Sciences(GJJSTD20200002)Xin Zhou acknowledges the support from the Tencent Foundation through the XPLORER PRIZE.
文摘Glycogen plays essential roles in glucose metabolism.Imaging glycogen in the liver,the major glycogen reservoir in the body,may shed new light on many metabolic disorders.^(13)C magnetic resonance spectroscopy(MRS)has become the mainstream method for monitoring glycogen in the body.However,the equipment of special hard-ware to standard clinical magnetic resonance imaging(MRI)scanners limits its clinical applications.Herein,we utilized endogenous glycogen as a T_(2)-based relaxation contrast agent for imaging glycogen metabolism in the liver in vivo.The in vitro results demonstrated that the transverse relaxation rate of glycogen strongly correlates with the concentration,pH,and field strength.Based on the Swift-Connick theory,we characterized the exchange property of glycogen and measured the exchange rate of glycogen as 31,847 Hz at 37°C.Besides,the viscosity and echo spacing showed no apparent effect on the transverse relaxation rate.This unique feature enables vi-sualization of glycogen signaling in vivo through T_(2)-weighted MRI.Two hours-post intraperitoneal injection of glucagon,a clinical drug to promote glycogenolysis and gluconeogenesis,the signal intensity of the mice’s liver increased by 1.8 times from the T_(2)-weighted imaging experiment due to the decomposition of glycogen.This study provides a convenient imaging strategy to non-invasively investigate glycogen metabolism in the liver,which may find clinical applications in metabolic diseases.
基金Supported by grants to G. S.: the National Key R&D Program of China (2019YFA0802600 and 2018YFC1004500)the National Natural Science Foundation of China (31725016, 31930019, and 91940303)and the Strategic Priority Research Program (Pilot Study) “Biological basis of aging and therapeutic strategies” of the Chinese Academy of Sciences (XDPB10).
文摘Mammalian mitochondria have small genomes encoding very limited numbers of proteins.Over one thousand proteins and noncoding RNAs encoded by the nuclear genome must be imported from the cytosol into the mitochondria.Here,we report the identification of hundreds of circular RNAs(mecciRNAs)encoded by the mitochondrial genome.We provide both in vitro and in vivo evidence to show that mecciRNAs facilitate the mitochondrial entry of nuclear-encoded proteins by serving as molecular chaperones in the folding of imported proteins.Known components involved in mitochondrial protein and RNA importation,such as TOM40 and PNPASE,interact with mecciRNAs and regulate protein entry.The expression of mecciRNAs is regulated,and these transcripts are critical for the adaption of mitochondria to physiological conditions and diseases such as stresses and cancers by modulating mitochondrial protein importation.mecciRNAs and their associated physiological roles add categories and functions to the known eukaryotic circular RNAs and shed novel light on the communication between mitochondria and the nucleus.
基金The authors wish to acknowledge the funding from National Postdoctoral Program for Innovative Talents(BX201700083)Introduction of the International Advanced Agricultural Science and Technology Program(948 plan)(Project No.2016-X24)+1 种基金Commonweal Project(Project No.201203059)13th Five-year Plan National Key Research Program(Project No.2017YFD0700503-2).
文摘Mechanized rice direct seeding is a cost-effective and efficient approach for rice cultivation.Recently,the use of rice direct seeding has been increasing rapidly owing to rural labour shortages and continuous increases in agricultural production costs.This article reviews the research and application progress of mechanized rice direct seeding including direct seeding technologies,precision rice seeding,precision rice seed-metering devices,key supporting agronomy technologies for mechanized rice direct seeding.South China Agricultural University developed precision rice hill-drop drilling(PRHDD)with synchronous furrowing and ridging technology and series machines for paddy that affords remarkable advantages in terms of saving time and labour,higher yield,and higher efficiency.In this approach,pre-germinated seeds are uniformly hill-dropped in the expected positions in puddled soil.It significantly improved the crop growth population and effectively solved the problems of high frequency of disease and pests caused by the irregular distribution of rice seeds with manual broadcasting,and generally reduces seed usage and increases the yield.Therefore,this technology has broad application prospects and great potential for promoting the development of mechanized rice direct seeding in China.
基金The authors acknowledge the funding from the Introduction of the National Key Research and Development Program of China(2018YFD0100800)the earmarked fund for China Agriculture Research System(CARS-01-41)+1 种基金International Advanced Agricultural Science and Technology Program(948 plan)(Project No.2016-X24)National Postdoctoral Program for Innovative Talents(BX201700083).
文摘To meet the requirement of the precision direct-seeding for hybrid rice,this study aimed to design a hill-drop pneumatic central cylinder direct-seeding machine to sow ten rows at a time.A series of orthogonal experiments were conducted to investigate the performance of the cylinder seeder.The influences of the hole diameters,degree of vacuum,and rotational speed of the cylinder were tested on JPS-12 computer-vision seeding test platform,and the rotational speed of 10-50 r/min,diameter of 135 mm and a negative pressure of 1.0-2.0 kPa were employed.Test results showed that the optimal parameter combination was a vacuum of 2.0 kPa and a hole diameter of 2.0 mm(straight hole),with a rotational speed of 30 r/min.The probability of(2±1)seeds in each hole was 95.3%,while the probability of seed-missing hole was 2.0%.A series of field experiments were then conducted to test the seeder performance according to China National Standard Test Methods,and the field test results showed that for the hill-drop pneumatic central cylinder direct-seeding machine,the probability of(2±1)seeds in each hill was 91.6%,while the probability of seed-missing hill was 2.7%.The yield data showed that the average effective panicle had 231.25 ears,the average seed setting rate was 9.92%,the average 1000-grain weight was 22.45 g,and the average yield was 7107.9 kg/hm^(2) that was 26.14%higher than the average yield of rice(5634.9 kg/hm^(2))of Guangdong Province in 2016.The results showed that the hill-drop pneumatic central cylinder direct-seeding machine for hybrid rice could be applied in practical precision rice seeding.
基金the National Natural Science Foundation of China(Grant 81974258,31870731,and 31971129)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB29030104)the Fundamental Research Fund for the Central Universities(WK3520000011,WK9110000092,and EF2060030006).
文摘Dear Editor,The spread of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)led to a global pandemic with 260 million infected people.A variety of vaccines,including the mRNA and recombinant vaccines,were developed to prevent the spread of the disease.1,2 There is an urgent need for testing the vaccine-induced long-term memory response based on the immunological principles and identifying the optimal combination of dose and injection manner to provide important information for clinical application.
