The positive regulation of bone-forming osteoblast activity and the negative feedback regulation of osteoclastic activity are equally important in strategies to achieve successful alveolar bone regeneration. Here, a m...The positive regulation of bone-forming osteoblast activity and the negative feedback regulation of osteoclastic activity are equally important in strategies to achieve successful alveolar bone regeneration. Here, a molybdenum(Mo)-containing bioactive glass ceramic scaffold with solid-strut-packed structures(Mo-scaffold) was printed, and its ability to regulate pro-osteogenic and antiosteoclastogenic cellular responses was evaluated in vitro and in vivo. We found that extracts derived from Mo-scaffold(Moextracts) strongly stimulated osteogenic differentiation of bone marrow mesenchymal stem cells and inhibited differentiation of osteoclast progenitors. The identified comodulatory effect was further demonstrated to arise from Mo ions in the Mo-extract,wherein Mo ions suppressed osteoclastic differentiation by scavenging reactive oxygen species(ROS) and inhibiting mitochondrial biogenesis in osteoclasts. Consistent with the in vitro findings, the Mo-scaffold was found to significantly promote osteoblastmediated bone formation and inhibit osteoclast-mediated bone resorption throughout the bone healing process, leading to enhanced bone regeneration. In combination with our previous finding that Mo ions participate in material-mediated immunomodulation, this study offers the new insight that Mo ions facilitate bone repair by comodulating the balance between bone formation and resorption. Our findings suggest that Mo ions are multifunctional cellular modulators that can potentially be used in biomaterial design and bone tissue engineering.展开更多
This study investigated the perceptions and medical history of third molars(M3s)and assessed the prevalence of visible M3s(V-M3s)among 904 Chinese adults.The enrolled participants were interviewed to complete a struct...This study investigated the perceptions and medical history of third molars(M3s)and assessed the prevalence of visible M3s(V-M3s)among 904 Chinese adults.The enrolled participants were interviewed to complete a structural questionnaire focused on sociodemographic information and their understanding of,attitudes toward,behaviors regarding,and medical history with respect to M3s.In addition,the number of V-M3s in the cohort was determined by oral examination.Logistic regression analysis was performed to explore the association between individuals'sociodemographic characteristics and their perception of M3s or the presence of V-M3s.The Chi-square test was used to compare the actions taken against symptomatic M3s and the corresponding outcomes among different groups divided according to respondents'sociodemographic factors.In total,904 completed questionnaires were gathered and analyzed.Nearly half(43.9%)of the respondents knew nothing about M3s,and only 12.7%provided correct answers to all the questions asked.Male sex,older age,occupation involving physical labor,and no previous dental experience were active factors in unawareness of M3s.Male sex was also significantly associated with the presence of at least one V-M3 and negative behavior about symptomatic M3s.In terms of medical history,192 participants reported having had at least one M3 extracted(438 in total),and 72.6%of the M3s were removed due to the presence of related symptoms or pathologies.In conclusion,the population investigated had a shortage of knowledge about M3s and adopted negative attitudes and actions about M3-related problems.展开更多
基金supported by the National Natural Science Foundation of China(grant nos.82130026,82001102 and 82170958)the Young Elite Scientist Support Program administered by CSA(2021PYRC001)the Natural Science Basic Research Program of Shaanxi(2020JQ-447)。
文摘The positive regulation of bone-forming osteoblast activity and the negative feedback regulation of osteoclastic activity are equally important in strategies to achieve successful alveolar bone regeneration. Here, a molybdenum(Mo)-containing bioactive glass ceramic scaffold with solid-strut-packed structures(Mo-scaffold) was printed, and its ability to regulate pro-osteogenic and antiosteoclastogenic cellular responses was evaluated in vitro and in vivo. We found that extracts derived from Mo-scaffold(Moextracts) strongly stimulated osteogenic differentiation of bone marrow mesenchymal stem cells and inhibited differentiation of osteoclast progenitors. The identified comodulatory effect was further demonstrated to arise from Mo ions in the Mo-extract,wherein Mo ions suppressed osteoclastic differentiation by scavenging reactive oxygen species(ROS) and inhibiting mitochondrial biogenesis in osteoclasts. Consistent with the in vitro findings, the Mo-scaffold was found to significantly promote osteoblastmediated bone formation and inhibit osteoclast-mediated bone resorption throughout the bone healing process, leading to enhanced bone regeneration. In combination with our previous finding that Mo ions participate in material-mediated immunomodulation, this study offers the new insight that Mo ions facilitate bone repair by comodulating the balance between bone formation and resorption. Our findings suggest that Mo ions are multifunctional cellular modulators that can potentially be used in biomaterial design and bone tissue engineering.
基金supported by the National Natural Science Foundation of China(Nos.82170958,81970947,and 81991503).
文摘This study investigated the perceptions and medical history of third molars(M3s)and assessed the prevalence of visible M3s(V-M3s)among 904 Chinese adults.The enrolled participants were interviewed to complete a structural questionnaire focused on sociodemographic information and their understanding of,attitudes toward,behaviors regarding,and medical history with respect to M3s.In addition,the number of V-M3s in the cohort was determined by oral examination.Logistic regression analysis was performed to explore the association between individuals'sociodemographic characteristics and their perception of M3s or the presence of V-M3s.The Chi-square test was used to compare the actions taken against symptomatic M3s and the corresponding outcomes among different groups divided according to respondents'sociodemographic factors.In total,904 completed questionnaires were gathered and analyzed.Nearly half(43.9%)of the respondents knew nothing about M3s,and only 12.7%provided correct answers to all the questions asked.Male sex,older age,occupation involving physical labor,and no previous dental experience were active factors in unawareness of M3s.Male sex was also significantly associated with the presence of at least one V-M3 and negative behavior about symptomatic M3s.In terms of medical history,192 participants reported having had at least one M3 extracted(438 in total),and 72.6%of the M3s were removed due to the presence of related symptoms or pathologies.In conclusion,the population investigated had a shortage of knowledge about M3s and adopted negative attitudes and actions about M3-related problems.
