Pu-erh tea, a traditional Chinese beverage, has been believed to have many benefits to human health and without side effects. In this study, we systematically analyzed the main active components of Pu-erh tea and inve...Pu-erh tea, a traditional Chinese beverage, has been believed to have many benefits to human health and without side effects. In this study, we systematically analyzed the main active components of Pu-erh tea and investigated its anti-obesity, anti-atherosclerotic and anti-oxidant effects using an obese rat model. Obesity was induced by feeding a high-fat diet and subsequently the experimental obese mice were fed with high-fat diet supplemented with low (2.5%), medium (5%) or high (7.5%) doses of Pu-erh tea powder for 6 weeks respectively. As result, the body weight gain of the rats was decreased by medium and high doses of Pu-erh tea treatments. Furthermore, the levels of serum total cholesterol (TC), triglyceride (TG) and atherosclerosis index (AI) were significantly lowered by Pu-erh tea compared to the control group. Conversely, high density lipoproteincholesterol (HDL-C) level of the rats was significantly elevated by Pu-erh tea treatments. In addition, Pu-erh tea treatments increased the activities of anti-oxidative enzymes such as superoxide dismutase (SOD) and glutathione peroxides (GSH-Px), whereas reduced the level of lipid peroxidation product malondialdehyde (MDA) in obese rats. Collectively, our find-ings revealed that Pu-erh tea exerts comprehensive benefits in anti-obesity, anti-atherosclerotic and anti-oxidant effects, therefore can be used as a promising functional food in obesity management.展开更多
In this study, 27 pairs of EST-SSR primers were employed to analyze the genetic diversity and genetic relationship of 100 wild tea plant germplasm re- sources and 22 cultivars, according to the results, a total of 88 ...In this study, 27 pairs of EST-SSR primers were employed to analyze the genetic diversity and genetic relationship of 100 wild tea plant germplasm re- sources and 22 cultivars, according to the results, a total of 88 polymorphic bands were amplified with 27 pairs of primers; the variation of effective alleles accounted for 69.01% ; a total of 183 genotypes were detected, with a variation range of 4 -11 ; averagely 6.78 genotypes were amplified with each primer pair; Shannon index （I） of 27 primer pairs ranged from 0.32 to I. 35, with an average of 0.88 ; the observed heterozygosity （0.52） was basically consistent with the expected het- erozygosity （0.52） ; the average polymorphism heterozygosity was 0.48, which was very close to 0.50 ; the average Nei＇s index was 0.51, which was higher than 0. 50 ; the average polymorphism information content （PIC） was 0.52, which was higher than 0.50, indicating high genetic diversity among wild tea germplasm resources in Yuunan Province. According to the clustering results, based on geographical origins and genetic backgrounds, 122 materials were clustered into 14 categories. Dendrogram based on Nei＇s genetic distance revealed complex genetic relationships among wild tea germplasm resources in Yunnan Province. This study provided certain reference for subsequent preservation, development and research of wild tea germplasm resources in China.展开更多
With 16 Yunnan tea tree varieties and 5 Kenya tea tree varieties as test materials,the differences in biochemical components between Yunnan and Kenya tea tree varieties were compared and analyzed.The results showed th...With 16 Yunnan tea tree varieties and 5 Kenya tea tree varieties as test materials,the differences in biochemical components between Yunnan and Kenya tea tree varieties were compared and analyzed.The results showed that the coefficients of variation of tea polyphenols,amino acids,caffeine,water extract,gallic acid(GA),catechin(C),epicatechin(EC),epicatechin gallate(ECG),epigallocatechin(EGC),epigallocatechin gallate(EGCG)and total catechins in Yunnan tea tree varieties were greater than those in Kenyan tea trees.The contents of tea polyphenols,amino acids,caffeine,water extract,C,EC,EGC,EGCG and total catechins in Yunnan tea tree varieties had no significant differences from those in Kenyan tea trees varieties(P>0.05),while there were significant differences in the contents of GA and ECG between Yunnan tea tree varieties and Kenya tea tree varieties(P<0.05).Therefore,it could be predicted that GA and ECG might be one of the main characteristics of the differences in biochemical components between Yunnan tea tree varieties and Kenyan tea tree varieties.The cluster analysis results showed that when the genetic distance was 15,the 21 tested tea varieties could be divided into three groups with obvious biochemical differences.展开更多
文摘Pu-erh tea, a traditional Chinese beverage, has been believed to have many benefits to human health and without side effects. In this study, we systematically analyzed the main active components of Pu-erh tea and investigated its anti-obesity, anti-atherosclerotic and anti-oxidant effects using an obese rat model. Obesity was induced by feeding a high-fat diet and subsequently the experimental obese mice were fed with high-fat diet supplemented with low (2.5%), medium (5%) or high (7.5%) doses of Pu-erh tea powder for 6 weeks respectively. As result, the body weight gain of the rats was decreased by medium and high doses of Pu-erh tea treatments. Furthermore, the levels of serum total cholesterol (TC), triglyceride (TG) and atherosclerosis index (AI) were significantly lowered by Pu-erh tea compared to the control group. Conversely, high density lipoproteincholesterol (HDL-C) level of the rats was significantly elevated by Pu-erh tea treatments. In addition, Pu-erh tea treatments increased the activities of anti-oxidative enzymes such as superoxide dismutase (SOD) and glutathione peroxides (GSH-Px), whereas reduced the level of lipid peroxidation product malondialdehyde (MDA) in obese rats. Collectively, our find-ings revealed that Pu-erh tea exerts comprehensive benefits in anti-obesity, anti-atherosclerotic and anti-oxidant effects, therefore can be used as a promising functional food in obesity management.
基金Supported by National Natural Science Foundation of China(31160175,31440034)Project for Protection and Utilization of Crop Germplasm Resources,Ministry of Agriculture(NB2012-2130135)+2 种基金Program of Technological Innovation Talents of Yunnan Province(2011CI068)Project for Construction of National Tea Modern Industrial Technology System of China(NYCYTX-23)Special Fund of Yunnan Academy of Agricultural Sciences(YAAS2012ZY002)
文摘In this study, 27 pairs of EST-SSR primers were employed to analyze the genetic diversity and genetic relationship of 100 wild tea plant germplasm re- sources and 22 cultivars, according to the results, a total of 88 polymorphic bands were amplified with 27 pairs of primers; the variation of effective alleles accounted for 69.01% ; a total of 183 genotypes were detected, with a variation range of 4 -11 ; averagely 6.78 genotypes were amplified with each primer pair; Shannon index （I） of 27 primer pairs ranged from 0.32 to I. 35, with an average of 0.88 ; the observed heterozygosity （0.52） was basically consistent with the expected het- erozygosity （0.52） ; the average polymorphism heterozygosity was 0.48, which was very close to 0.50 ; the average Nei＇s index was 0.51, which was higher than 0. 50 ; the average polymorphism information content （PIC） was 0.52, which was higher than 0.50, indicating high genetic diversity among wild tea germplasm resources in Yuunan Province. According to the clustering results, based on geographical origins and genetic backgrounds, 122 materials were clustered into 14 categories. Dendrogram based on Nei＇s genetic distance revealed complex genetic relationships among wild tea germplasm resources in Yunnan Province. This study provided certain reference for subsequent preservation, development and research of wild tea germplasm resources in China.
基金Supported by Major Science and Technology Project in Yunnan Province(2018ZG009).
文摘With 16 Yunnan tea tree varieties and 5 Kenya tea tree varieties as test materials,the differences in biochemical components between Yunnan and Kenya tea tree varieties were compared and analyzed.The results showed that the coefficients of variation of tea polyphenols,amino acids,caffeine,water extract,gallic acid(GA),catechin(C),epicatechin(EC),epicatechin gallate(ECG),epigallocatechin(EGC),epigallocatechin gallate(EGCG)and total catechins in Yunnan tea tree varieties were greater than those in Kenyan tea trees.The contents of tea polyphenols,amino acids,caffeine,water extract,C,EC,EGC,EGCG and total catechins in Yunnan tea tree varieties had no significant differences from those in Kenyan tea trees varieties(P>0.05),while there were significant differences in the contents of GA and ECG between Yunnan tea tree varieties and Kenya tea tree varieties(P<0.05).Therefore,it could be predicted that GA and ECG might be one of the main characteristics of the differences in biochemical components between Yunnan tea tree varieties and Kenyan tea tree varieties.The cluster analysis results showed that when the genetic distance was 15,the 21 tested tea varieties could be divided into three groups with obvious biochemical differences.
