The dominant frequency modes of pre-summer extreme precipitation events(EPEs)over South China(SC)between1998 and 2018 were investigated.The 67 identified EPEs were all characterized by the 3-8-d(synoptic)frequency ban...The dominant frequency modes of pre-summer extreme precipitation events(EPEs)over South China(SC)between1998 and 2018 were investigated.The 67 identified EPEs were all characterized by the 3-8-d(synoptic)frequency band.However,multiscale combined modes of the synoptic and three low-frequency bands[10-20-d(quasi-biweekly,QBW);15-40-d(quasi-monthly,QM);and 20-60-d(intraseasonal)]accounted for the majority(63%)of the EPEs,and the precipitation intensity on the peak wet day was larger than that of the single synoptic mode.It was found that EPEs form within strong southwesterly anomalous flows characterized by either lower-level cyclonic circulation over SC or a deep trough over eastern China.Bandpass-filtered disturbances revealed the direct precipitating systems and their life cycles.Synoptic-scale disturbances are dominated by mid-high latitude troughs,and the cyclonic anomalies originate from downstream of the Tibetan Plateau(TP).Given the warm and moist climate state,synoptic-scale northeasterly flows can even induce EPEs.At the QBW and QM scales,the disturbances originate from the tropical Pacific,downstream of the TP,or mid-high latitudes(QBW only).Each is characterized by cyclonic-anticyclonic wave trains and intense southwesterly flows between them within a region of large horizontal pressure gradient.The intraseasonal disturbances are confined to tropical regions and influence SC by marginal southwesterly flows.It is concluded that low-frequency disturbances provide favorable background conditions for EPEs over SC and synoptic-scale disturbances ultimately induce EPEs on the peak wet days.Both should be simultaneously considered for EPE predictions over SC.展开更多
A method is proposed to fuse the velocity data of the global navigation satellite system(GNSS) and leveling height via combined adjustment with constraints. First, stable GNSS-leveling points are uniformly selected, a...A method is proposed to fuse the velocity data of the global navigation satellite system(GNSS) and leveling height via combined adjustment with constraints. First, stable GNSS-leveling points are uniformly selected, and the constraints of the geodetic height change velocity and normal height change velocity are given. Then, the GNSS vertical velocities and leveling height difference are used as observations of combined adjustment, and robust least-squares estimation are used to estimate the velocities of the unknown points. Finally, a vertical movement model is established with the GNSS vertical velocities and leveling vertical velocities obtained via combined adjustment. Data from the second-order leveling network and GNSS control points in Shandong Province are taken as test data, and eight calculation schemes are used for discussion. One of the schemes, the bifactor robust combined adjustment method based on variance component estimation with two kinds of vertical velocity constraints achieves the optimal results. The method applied in the scheme can be recommended for data fusion of GNSS and leveling, further improving the reliability of vertical crustal movement in Shandong Province.展开更多
Glioblastoma(GBM)is a lethal cancer with limited therapeutic options.Dendritic cell(DC)-based cancer vaccines provide a promising approach for GBM treatment.Clinical studies suggest that other immunotherapeutic agents...Glioblastoma(GBM)is a lethal cancer with limited therapeutic options.Dendritic cell(DC)-based cancer vaccines provide a promising approach for GBM treatment.Clinical studies suggest that other immunotherapeutic agents may be combined with DC vaccines to further enhance antitumor activity.Here,we report a GBM case with combination immunotherapy consisting of DC vaccines,anti-programmed death-1(anti-PD-1)and poly I:C as well as the chemotherapeutic agent cyclophosphamide that was integrated with standard chemoradiation therapy,and the patient remained disease-free for 69 months.The patient received DC vaccines loaded with multiple forms of tumor antigens,including mRNA-tumor associated antigens(TAA),mRNA-neoantigens,and hypochlorous acid(HOCl)-oxidized tumor lysates.Furthermore,mRNA-TAAs were modified with a novel TriVac technology that fuses TAAs with a destabilization domain and inserts TAAs into full-length lysosomal associated membrane protein-1 to enhance major histocompatibility complex(MHC)class I and II antigen presentation.The treatment consisted of 42 DC cancer vaccine infusions,26 anti-PD-1 antibody nivolumab administrations and 126 poly I:C injections for DC infusions.The patient also received 28 doses of cyclophosphamide for depletion of regulatory T cells.No immunotherapy-related adverse events were observed during the treatment.Robust antitumor CD4t and CD8t T-cell responses were detected.The patient remains free of disease progression.This is the first case report on the combination of the above three agents to treat glioblastoma patients.Our results suggest that integrated combination immunotherapy is safe and feasible for long-term treatment in this patient.A large-scale trial to validate these findings is warranted.展开更多
Sugarcane leaves-derived polyphenols(SLP)have been demonstrated to have diverse health-promoting benefits,but the mechanism of action has not been fully elucidated.This study aimed to investigate the anti-metabolic di...Sugarcane leaves-derived polyphenols(SLP)have been demonstrated to have diverse health-promoting benefits,but the mechanism of action has not been fully elucidated.This study aimed to investigate the anti-metabolic disease effects of SLP and the underlying mechanisms in mice.In the current study,we prepared the SLP mainly consisting of three flavonoid glycosides,three phenol derivatives,and two lignans including one new compound,and further demonstrated that SLP reduced body weight gain and fat accumulation,improved glucose and lipid metabolism disorders,ameliorated hepatic steatosis,and regulated short-chain fatty acids(SCFAs)production and secondary bile acids metabolism in ob/ob mice.Notably,SLP largely altered the gut microbiota composition,especially enriching the commensal bacteria Akkermansia muciniphila and Bacteroides acidifaciens.Oral gavage with the above two strains ameliorated metabolic syndrome(MetS),regulated secondary bile acid metabolism,and increased the production of SCFAs in high-fat diet(HFD)-induced obese mice.These results demonstrated that SLP could be used as a prebiotic to attenuate MetS via regulating gut microbiota composition and further activating the secondary bile acids-mediated gut-adipose axis.展开更多
Systemically angular and planar transport investigations are performed in layered antiferromagnetic(AF)V_(5)S_(8).In this AF system,obvious anomalous Hall effect(AHE)is observed with a large Hall angle of 0.1 compared...Systemically angular and planar transport investigations are performed in layered antiferromagnetic(AF)V_(5)S_(8).In this AF system,obvious anomalous Hall effect(AHE)is observed with a large Hall angle of 0.1 compared to that in ferromagnetic(FM)system.It can persist to the temperatures above AF transition and exhibit strong angular field dependence.The phase diagram reveals various magnetic states by rotating the applied field.By analyzing the anisotropic transport behavior,magnon contributions are revealed and exhibit obvious angular dependence with a spin-flop vanishing line.The observed prominent planar Hall effect and anisotropic magnetoresisitivity exhibit two-fold systematical angular dependent oscillations.These behaviors are attributed to the scattering from spin–orbital coupling instead of nontrivial topological origin.Our results reveal anisotropic interactions of magnetism and electron in V5S8,suggesting potential opportunities for the AF spintronic sensor and devices.展开更多
The role of lncRNA KCNQ1 opposite strand/antisense transcript 1(KCNQ1OT1)in colon cancer involves various tumorigenic processes and has been studed widely.However,the mechanism by which it promotes colon cancer remain...The role of lncRNA KCNQ1 opposite strand/antisense transcript 1(KCNQ1OT1)in colon cancer involves various tumorigenic processes and has been studed widely.However,the mechanism by which it promotes colon cancer remains unclear.Retrovirnl vector pSEB61 was retroftted in established HCT116 siKCN and SW480-siKCN cells to silence KCNQ1 OT1.Cellular proliferation was measured using CCK8 assay,and flow cytometry(FCM)detected cell cydle changes.RNA sequencing(RNA Seq)analysis showed differentially expressed genes(DEGs).Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were carried out to analyze enriched functions and signaling pathways.RT-qPCR,immunofluorescence,and western blotting were carried out to validate downstream gene expressions.The effects of tumorigenesis were evaluated in BALB/c nude mice by tumor xenografts.Our data revealed that the silencing of KONQ1OT1 in HCT116 and SW480 cells slowed cell growth and decreased the number of cells in the G2/M phase.RNA-Seq analysis showed the data of DEGs enriched in various GO and KEGG pathways such as DNA replication and cell cyde.RT qPCR,immunofluorescence,and western blotting confirmed downstream CCNE2 and PCNA gene expressions.HCT116 siKCN cells signifcantly suppressed tumorigenesis in BALB/c nude mice.Our study suggests that lncRNA KCNQ1OT1 may provide a promising therapeutic strategy for colon cancer.展开更多
Medicinal leeches have been utilized in therapy for thousands of years. However, the adaptation physiology between leeches and hosts is not fully understand. To disclose the molecular mechanisms of adaptation between ...Medicinal leeches have been utilized in therapy for thousands of years. However, the adaptation physiology between leeches and hosts is not fully understand. To disclose the molecular mechanisms of adaptation between leech and host, the body transcriptomes of hunger and fed blood-sucking Poecilobdella javanica, Haemadipsa cavatuses, and Hirudo nipponia leeches were obtained by RNA sequencing, after comparison, a stratified unigenes group was obtained, which closely correlated to body distension. In the group, Rfamide receptor decreased significantly (P < 0.05) while serotonin receptor increased significantly (P < 0.05). Moreover, four KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, including cardiac muscle contraction, complement and coagulation cascades, renin-angiotensin system, and hypertrophic cardiomyopathy were significantly enriched. The unigenes annotation, neuroregulators correlation analysis and induced function of the KEGG pathways, were consistently supported the same result as: vasoconstriction and systole reaction enhance in hunger leeches and vice versa vasodilation and diastole increase in fed leeches, meanwhile, Interspecific comparison and correlative analyses of physiological function showed that the strongest reaction of induced heart failure from four KEGG occur in strongest reaction of systole in hungry P. javanica and in strongest reaction of diastole in fed H. nipponia. Overall, heart failure is likely a physiological function involved in feeding behaviour.展开更多
The degradation of acephate in aqueous solutions was investigated with the ultrasonic and ozonation methods, as well as a combination of both. An experimental facility was designed and operation parameters such as the...The degradation of acephate in aqueous solutions was investigated with the ultrasonic and ozonation methods, as well as a combination of both. An experimental facility was designed and operation parameters such as the ultrasonic power, temperature, and gas flow rate were strictly controlled at constant levels. The frequency of the ultrasonic wave was 160 k Hz. The ultraviolet-visible(UV-Vis) spectroscopic and Raman spectroscopic techniques were used in the experiment. The UV-Vis spectroscopic results show that ultrasonication and ozonation have a synergistic effect in the combined system. The degradation efficiency of acephate increases from 60.6% to 87.6% after the solution is irradiated by a160 k Hz ultrasonic wave for 60 min in the ozonation process, and it is higher with the combined method than the sum of the separated ultrasonic and ozonation methods. Raman spectra studies show that degradation via the combined ultrasonic/ozonation method is more thorough than photocatalysis. The oxidability of nitrogen atoms is promoted under ultrasonic waves. Changes of the inorganic ions and degradation pathway during the degradation process were investigated in this study. Most final products are innocuous to the environment.展开更多
Objective: To study the effect of lipoteichoic acid(LTA) and 5-FU on the expression of caspase-3, EGFR, TGF-α proteins of tumor tissue of H22 cancer bearing mice and its antitumor mechanism. Methods: A total of 40 SP...Objective: To study the effect of lipoteichoic acid(LTA) and 5-FU on the expression of caspase-3, EGFR, TGF-α proteins of tumor tissue of H22 cancer bearing mice and its antitumor mechanism. Methods: A total of 40 SPF grade Kunming mice were selected to establish H22 liver cancer model, and then the mice were divided into 4 groups at random with ten mice in each group. Group A was given saline lavage treatment, Group B was treated with 5-FU by intraperitomeal injection, Group C was treated with LTA by lump body injection; Group D was treated with LTA by lump body injection and 5-FU by intraperitomeal injection. Two weeks after the treatment, the mice in each group were executed and the tumor tissue was stripping and weighted, and the tumor growth inhibition ratio was calculated. Then the tumor tissue was processed for conventional embedding, sectioned to observe the expression of caspase-3, EGFR, TGF-α by immunohistochemical staining method. Results: The tumor inhibitory rate o f Group D was significantly higher than Groups B and C(P<0.05); B, the tumor inhibitory rate o f Group B had no statistical difference compared with Group C(P>0.05). The IDO values of TGF-α, EGFR proteins in Groups B, C, D mice tumor tissue were significantly lower than that in group A(P<0.05); while IDO value of caspase-3 in Groups B, C, D group mice tumor tissue was significantly higher than that in Group A(P<0.05). The IDO value of TGF-α, EGFR in Group D mice tumor tissue were significantly lower than that in Groups B and C; While IDO value of aspase-3 in Group D was significantly higher than that in Groups B and C(P<0.05). Conclusions: LTA combined with 5-FU can effectively inhibit the tumorigenesis of H22 tumor bearing mice, increase the caspase-3 protein expression, inhibit TGF –α and EGFR protein expression, further promote tumor cell apoptosis and play a synergistic antitumor effect.展开更多
Sea-water-level(SWL)prediction significantly impacts human lives and maritime activities in coastal regions,particularly at offshore locations with shallow water levels.Long-term SWL forecasts,which are conventionally...Sea-water-level(SWL)prediction significantly impacts human lives and maritime activities in coastal regions,particularly at offshore locations with shallow water levels.Long-term SWL forecasts,which are conventionally obtained via harmonic analysis,become ineffective when nonperiodic meteorological events predominate.Artificial intelligence combined with other data-processing methods can effectively forecast highly nonlinear and nonstationary inflow patterns by recognizing historical relationships between input and output.These techniques are considerably useful in time-series data predictions.This paper reports the development of a hybrid model to realize accurate multihour SWL forecasting by combining an adaptive neuro-fuzzy inference system(ANFIS)with wavelet decomposition while using sea-level anomaly(SLA)and wind-shear-velocity components as inputs.Numerous wavelet-ANFIS(WANFIS)models have been tested using different inputs to assess their applicability as alternatives to the artificial neural network(ANN),wavelet ANN(WANN),and ANFIS models.Different error definitions have been used to evaluate results,which indicate that integrated wavelet-decomposition and ANFIS models improve the accuracy of SWL prediction and that the inputs of SLA and wind-shear velocity exhibit superior prediction capability compared to conventional SWL-only models.展开更多
Objective:To systematically evaluate the safety and effectiveness of Qishen Yiqi dripping pills combined with trimetazidine in the treatment of chronic heart failure.Methods:A total of four English and Chinese electro...Objective:To systematically evaluate the safety and effectiveness of Qishen Yiqi dripping pills combined with trimetazidine in the treatment of chronic heart failure.Methods:A total of four English and Chinese electronic databases were searched:CNKI,VIP,CBM,PUBMED.Cochrane bias risk tools were used to assess the methodological quality of qualified studies.Meta-analysis was conducted by Review Manager 5.3.A total of 9 articles were included,with a total sample size of 855 cases,443 cases in the observation group and 412 cases in the treatment group.Results:Qishen Yiqi dripping pills combined with trimetazidine in the treatment of chronic heart failure has a total effective rate(RR=1.22,95%CI[1.15-1.30];p<0.00001),LVEF(MD=6.03,95%CI[5.39,6.67],P<0.00001),BNP(MD=-101.87,95%CI[-109.90,-93.83],P<0.00001),E/A(MD=-4.32,95%CI[-5.70,-2.93],P<0.00001),SYP(MD=-10.32,95%CI[-13.32,-7.32],P<0.00001),LVESD(MD=-5.50,95%CI[-6.03,-4.96],P<0.00001),6-MWT(MD=110.13,95%CI[96.89,123.36],P<0.00001)Adverse reactions occurred less frequently and did not affect treatment.Conclusion:This study shows that QYDP combined with TMZ can improve various indicators of CHF patients.However,due to the small sample size and the generally low quality of research,a more rigorous and reasonably designed RCT is needed to confirm these findings.展开更多
Objective:To investigate the effect of dexmedetomidine combined with ultrasound guided nerve block on the hemodynamics, immune function and cortisol level in the patients undergoing inguinal surgery.Methods:A total of...Objective:To investigate the effect of dexmedetomidine combined with ultrasound guided nerve block on the hemodynamics, immune function and cortisol level in the patients undergoing inguinal surgery.Methods:A total of 120 patients who underwent unilateral inguinal surgery in our hospital were randomly divided into control group and observation group, 60 cases in each group. The control group was induced by intravenous anesthesia with sufentanil. The observation group was given dexmedetomidine combined with ultrasound guided ilioinguinal/iliac hypogastric nerve block. Hemodynamics, immune function and cortisol levels were compared between the two groups before and after anesthesia induction. Results:After anesthesia, in surgical incision and recovery time, the levels of HR in the two groups were significantly lower than that before the anesthesia, and the levels of HR in the observation group were significantly lower than that in the control group. After anesthesia, in surgical incision and recovery time, the levels of CD3+, CD4+ and CD4+/CD8+ in the control group were significantly lower than that of the group before anesthesia. After anesthesia, the levels of CD3+, CD4+ and CD4+/CD8+ in the observation group were significantly lower than that of the group before anesthesia. In surgical incision and recovery time, there was no significant difference in the levels of CD3+, CD4+ and CD4+/CD8+ in the observation group before anesthesia. After anesthesia, in surgical incision and recovery time, the levels of CD3+, CD4+ and CD4+/CD8+ in the observation group were significantly higher than that of the control group. The levels of serum PI3K, ET -1, CRP and cortisol of the two groups were significantly higher than those before anesthesia, and the levels of PI3K, ET -1, CRP and cortisol in the serum of the patients in the observation group were significantly lower than those in the control group.Conclusion: The effect of dexmedetomidin combined with ultrasound guided nerve block anesthesia on the patients with inguinal surgery can effectively maintain the hemodynamic stability of the patients and have less influence on the immune function and the stress response. The security is higher.展开更多
This article summarizes the progress made in predictability studies of weather and climate in recent years in China,with a main focus on advances in methods to study error growth dynamics and reduce uncertainties in t...This article summarizes the progress made in predictability studies of weather and climate in recent years in China,with a main focus on advances in methods to study error growth dynamics and reduce uncertainties in the forecasting of weather and climate.Specifically,it covers(a)advances in methods to study weather and climate predictability dynamics,especially those in nonlinear optimal perturbation methods associated with initial errors and model errors and their applications to ensemble forecasting and target observations,(b)new data assimilation algorithms for initialization of predictions and novel assimilation approaches to neutralize the combined effects of initial and model errors for weather and climate,(c)applications of new statistical approaches to climate predictions,and(d)studies on meso-to small-scale weather system predictability dynamics.Some of the major frontiers and challenges remaining in predictability studies are addressed in this context.展开更多
基金supported by the National Key R&D Program of China(Grant No.2018YFC1507403)。
文摘The dominant frequency modes of pre-summer extreme precipitation events(EPEs)over South China(SC)between1998 and 2018 were investigated.The 67 identified EPEs were all characterized by the 3-8-d(synoptic)frequency band.However,multiscale combined modes of the synoptic and three low-frequency bands[10-20-d(quasi-biweekly,QBW);15-40-d(quasi-monthly,QM);and 20-60-d(intraseasonal)]accounted for the majority(63%)of the EPEs,and the precipitation intensity on the peak wet day was larger than that of the single synoptic mode.It was found that EPEs form within strong southwesterly anomalous flows characterized by either lower-level cyclonic circulation over SC or a deep trough over eastern China.Bandpass-filtered disturbances revealed the direct precipitating systems and their life cycles.Synoptic-scale disturbances are dominated by mid-high latitude troughs,and the cyclonic anomalies originate from downstream of the Tibetan Plateau(TP).Given the warm and moist climate state,synoptic-scale northeasterly flows can even induce EPEs.At the QBW and QM scales,the disturbances originate from the tropical Pacific,downstream of the TP,or mid-high latitudes(QBW only).Each is characterized by cyclonic-anticyclonic wave trains and intense southwesterly flows between them within a region of large horizontal pressure gradient.The intraseasonal disturbances are confined to tropical regions and influence SC by marginal southwesterly flows.It is concluded that low-frequency disturbances provide favorable background conditions for EPEs over SC and synoptic-scale disturbances ultimately induce EPEs on the peak wet days.Both should be simultaneously considered for EPE predictions over SC.
基金supported by the National Natural Science Foundation of China(41774004,41904040)the Technological Innovation of SHASG(SCK2020-11).
文摘A method is proposed to fuse the velocity data of the global navigation satellite system(GNSS) and leveling height via combined adjustment with constraints. First, stable GNSS-leveling points are uniformly selected, and the constraints of the geodetic height change velocity and normal height change velocity are given. Then, the GNSS vertical velocities and leveling height difference are used as observations of combined adjustment, and robust least-squares estimation are used to estimate the velocities of the unknown points. Finally, a vertical movement model is established with the GNSS vertical velocities and leveling vertical velocities obtained via combined adjustment. Data from the second-order leveling network and GNSS control points in Shandong Province are taken as test data, and eight calculation schemes are used for discussion. One of the schemes, the bifactor robust combined adjustment method based on variance component estimation with two kinds of vertical velocity constraints achieves the optimal results. The method applied in the scheme can be recommended for data fusion of GNSS and leveling, further improving the reliability of vertical crustal movement in Shandong Province.
基金supported by Natural Science Foundation of Shaanxi Province(Grant No.:2019ZY-CXPT-03-01)to Ping Zhu and Key Research and Development Program of Shaanxi Province(Grant No.:2020ZDLSF03-02)to Zhi-Nan Chen and Huijie Bian as well as Tricision Biotherapeutics Inc.
文摘Glioblastoma(GBM)is a lethal cancer with limited therapeutic options.Dendritic cell(DC)-based cancer vaccines provide a promising approach for GBM treatment.Clinical studies suggest that other immunotherapeutic agents may be combined with DC vaccines to further enhance antitumor activity.Here,we report a GBM case with combination immunotherapy consisting of DC vaccines,anti-programmed death-1(anti-PD-1)and poly I:C as well as the chemotherapeutic agent cyclophosphamide that was integrated with standard chemoradiation therapy,and the patient remained disease-free for 69 months.The patient received DC vaccines loaded with multiple forms of tumor antigens,including mRNA-tumor associated antigens(TAA),mRNA-neoantigens,and hypochlorous acid(HOCl)-oxidized tumor lysates.Furthermore,mRNA-TAAs were modified with a novel TriVac technology that fuses TAAs with a destabilization domain and inserts TAAs into full-length lysosomal associated membrane protein-1 to enhance major histocompatibility complex(MHC)class I and II antigen presentation.The treatment consisted of 42 DC cancer vaccine infusions,26 anti-PD-1 antibody nivolumab administrations and 126 poly I:C injections for DC infusions.The patient also received 28 doses of cyclophosphamide for depletion of regulatory T cells.No immunotherapy-related adverse events were observed during the treatment.Robust antitumor CD4t and CD8t T-cell responses were detected.The patient remains free of disease progression.This is the first case report on the combination of the above three agents to treat glioblastoma patients.Our results suggest that integrated combination immunotherapy is safe and feasible for long-term treatment in this patient.A large-scale trial to validate these findings is warranted.
基金supported by the National key research and development program of China(2019YFA0905600)the Science and Technology Service Network Program of the Chinese Academy of Sciences(KFJ-STS-QYZD-201-5-3)the Strategic Priority Research Program(Class B)of Chinese Academy of Sciences(XDB 38020300)。
文摘Sugarcane leaves-derived polyphenols(SLP)have been demonstrated to have diverse health-promoting benefits,but the mechanism of action has not been fully elucidated.This study aimed to investigate the anti-metabolic disease effects of SLP and the underlying mechanisms in mice.In the current study,we prepared the SLP mainly consisting of three flavonoid glycosides,three phenol derivatives,and two lignans including one new compound,and further demonstrated that SLP reduced body weight gain and fat accumulation,improved glucose and lipid metabolism disorders,ameliorated hepatic steatosis,and regulated short-chain fatty acids(SCFAs)production and secondary bile acids metabolism in ob/ob mice.Notably,SLP largely altered the gut microbiota composition,especially enriching the commensal bacteria Akkermansia muciniphila and Bacteroides acidifaciens.Oral gavage with the above two strains ameliorated metabolic syndrome(MetS),regulated secondary bile acid metabolism,and increased the production of SCFAs in high-fat diet(HFD)-induced obese mice.These results demonstrated that SLP could be used as a prebiotic to attenuate MetS via regulating gut microbiota composition and further activating the secondary bile acids-mediated gut-adipose axis.
基金Project supported by the open research fund of Songshan Lake Materials Laboratory(Grant No.2021SLABFN11)the National Natural Science Foundation of China(Grant Nos.U2130101 and 92165204)+5 种基金Natural Science Foundation of Guangdong Province(Grant No.2022A1515010035)Guangzhou Basic and Applied Basic Research Foundation(Grant No.202201011798)the Open Project of Guangdong Provincial Key Laboratory of Magnetoelectric Physics and Devices(Grant No.2022B1212010008)the Open Project of Key Laboratory of Optoelectronic Materials and Technologies(Grant No.OEMT-2023-ZTS-01)the National Key R&D Program of China(Grant Nos.2023YFF0718400 and 2023YFA1406500)(national)college students innovation and entrepreneurship training program,Sun Yat-sen University(Grant No.202310359).
文摘Systemically angular and planar transport investigations are performed in layered antiferromagnetic(AF)V_(5)S_(8).In this AF system,obvious anomalous Hall effect(AHE)is observed with a large Hall angle of 0.1 compared to that in ferromagnetic(FM)system.It can persist to the temperatures above AF transition and exhibit strong angular field dependence.The phase diagram reveals various magnetic states by rotating the applied field.By analyzing the anisotropic transport behavior,magnon contributions are revealed and exhibit obvious angular dependence with a spin-flop vanishing line.The observed prominent planar Hall effect and anisotropic magnetoresisitivity exhibit two-fold systematical angular dependent oscillations.These behaviors are attributed to the scattering from spin–orbital coupling instead of nontrivial topological origin.Our results reveal anisotropic interactions of magnetism and electron in V5S8,suggesting potential opportunities for the AF spintronic sensor and devices.
基金the Scientific Research Project of Anhui Provincial Health Commission in 2021(#AHWJ2021b109 to LS)Scientific and Technological Research Program of Chongqing Municipal Education Commission(#KJZD-K201900402 to TZ)+1 种基金Special Fund for Wannan Medical College Scholar Project(#WK2021F07)Educational Commission of Anhui Province of China(2022AH051241).
文摘The role of lncRNA KCNQ1 opposite strand/antisense transcript 1(KCNQ1OT1)in colon cancer involves various tumorigenic processes and has been studed widely.However,the mechanism by which it promotes colon cancer remains unclear.Retrovirnl vector pSEB61 was retroftted in established HCT116 siKCN and SW480-siKCN cells to silence KCNQ1 OT1.Cellular proliferation was measured using CCK8 assay,and flow cytometry(FCM)detected cell cydle changes.RNA sequencing(RNA Seq)analysis showed differentially expressed genes(DEGs).Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were carried out to analyze enriched functions and signaling pathways.RT-qPCR,immunofluorescence,and western blotting were carried out to validate downstream gene expressions.The effects of tumorigenesis were evaluated in BALB/c nude mice by tumor xenografts.Our data revealed that the silencing of KONQ1OT1 in HCT116 and SW480 cells slowed cell growth and decreased the number of cells in the G2/M phase.RNA-Seq analysis showed the data of DEGs enriched in various GO and KEGG pathways such as DNA replication and cell cyde.RT qPCR,immunofluorescence,and western blotting confirmed downstream CCNE2 and PCNA gene expressions.HCT116 siKCN cells signifcantly suppressed tumorigenesis in BALB/c nude mice.Our study suggests that lncRNA KCNQ1OT1 may provide a promising therapeutic strategy for colon cancer.
文摘Medicinal leeches have been utilized in therapy for thousands of years. However, the adaptation physiology between leeches and hosts is not fully understand. To disclose the molecular mechanisms of adaptation between leech and host, the body transcriptomes of hunger and fed blood-sucking Poecilobdella javanica, Haemadipsa cavatuses, and Hirudo nipponia leeches were obtained by RNA sequencing, after comparison, a stratified unigenes group was obtained, which closely correlated to body distension. In the group, Rfamide receptor decreased significantly (P < 0.05) while serotonin receptor increased significantly (P < 0.05). Moreover, four KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, including cardiac muscle contraction, complement and coagulation cascades, renin-angiotensin system, and hypertrophic cardiomyopathy were significantly enriched. The unigenes annotation, neuroregulators correlation analysis and induced function of the KEGG pathways, were consistently supported the same result as: vasoconstriction and systole reaction enhance in hunger leeches and vice versa vasodilation and diastole increase in fed leeches, meanwhile, Interspecific comparison and correlative analyses of physiological function showed that the strongest reaction of induced heart failure from four KEGG occur in strongest reaction of systole in hungry P. javanica and in strongest reaction of diastole in fed H. nipponia. Overall, heart failure is likely a physiological function involved in feeding behaviour.
基金supported by the National Natural Science Foundation of China(Grants No.11274092,11274091,and 11304026)the Jiangsu Graduate Education Reform Research and Practice Project in 2009(Grant No.22)the Fundamental Research Fund for the Central Universities(Grant No.14B10128)
文摘The degradation of acephate in aqueous solutions was investigated with the ultrasonic and ozonation methods, as well as a combination of both. An experimental facility was designed and operation parameters such as the ultrasonic power, temperature, and gas flow rate were strictly controlled at constant levels. The frequency of the ultrasonic wave was 160 k Hz. The ultraviolet-visible(UV-Vis) spectroscopic and Raman spectroscopic techniques were used in the experiment. The UV-Vis spectroscopic results show that ultrasonication and ozonation have a synergistic effect in the combined system. The degradation efficiency of acephate increases from 60.6% to 87.6% after the solution is irradiated by a160 k Hz ultrasonic wave for 60 min in the ozonation process, and it is higher with the combined method than the sum of the separated ultrasonic and ozonation methods. Raman spectra studies show that degradation via the combined ultrasonic/ozonation method is more thorough than photocatalysis. The oxidability of nitrogen atoms is promoted under ultrasonic waves. Changes of the inorganic ions and degradation pathway during the degradation process were investigated in this study. Most final products are innocuous to the environment.
基金supported by Applied Basic Research Program,Science and Technology Bureau of Wuhan City,(No.2013062301010823)Medical Care and Science Research Project of Health and Family Planning Commission of Wuhan(No.WX14A11,WX15D26)The third group of"Hanyang Talents’Plan"
文摘Objective: To study the effect of lipoteichoic acid(LTA) and 5-FU on the expression of caspase-3, EGFR, TGF-α proteins of tumor tissue of H22 cancer bearing mice and its antitumor mechanism. Methods: A total of 40 SPF grade Kunming mice were selected to establish H22 liver cancer model, and then the mice were divided into 4 groups at random with ten mice in each group. Group A was given saline lavage treatment, Group B was treated with 5-FU by intraperitomeal injection, Group C was treated with LTA by lump body injection; Group D was treated with LTA by lump body injection and 5-FU by intraperitomeal injection. Two weeks after the treatment, the mice in each group were executed and the tumor tissue was stripping and weighted, and the tumor growth inhibition ratio was calculated. Then the tumor tissue was processed for conventional embedding, sectioned to observe the expression of caspase-3, EGFR, TGF-α by immunohistochemical staining method. Results: The tumor inhibitory rate o f Group D was significantly higher than Groups B and C(P<0.05); B, the tumor inhibitory rate o f Group B had no statistical difference compared with Group C(P>0.05). The IDO values of TGF-α, EGFR proteins in Groups B, C, D mice tumor tissue were significantly lower than that in group A(P<0.05); while IDO value of caspase-3 in Groups B, C, D group mice tumor tissue was significantly higher than that in Group A(P<0.05). The IDO value of TGF-α, EGFR in Group D mice tumor tissue were significantly lower than that in Groups B and C; While IDO value of aspase-3 in Group D was significantly higher than that in Groups B and C(P<0.05). Conclusions: LTA combined with 5-FU can effectively inhibit the tumorigenesis of H22 tumor bearing mice, increase the caspase-3 protein expression, inhibit TGF –α and EGFR protein expression, further promote tumor cell apoptosis and play a synergistic antitumor effect.
基金The National Key R&D Program of China under contract No.2016YFC1402609。
文摘Sea-water-level(SWL)prediction significantly impacts human lives and maritime activities in coastal regions,particularly at offshore locations with shallow water levels.Long-term SWL forecasts,which are conventionally obtained via harmonic analysis,become ineffective when nonperiodic meteorological events predominate.Artificial intelligence combined with other data-processing methods can effectively forecast highly nonlinear and nonstationary inflow patterns by recognizing historical relationships between input and output.These techniques are considerably useful in time-series data predictions.This paper reports the development of a hybrid model to realize accurate multihour SWL forecasting by combining an adaptive neuro-fuzzy inference system(ANFIS)with wavelet decomposition while using sea-level anomaly(SLA)and wind-shear-velocity components as inputs.Numerous wavelet-ANFIS(WANFIS)models have been tested using different inputs to assess their applicability as alternatives to the artificial neural network(ANN),wavelet ANN(WANN),and ANFIS models.Different error definitions have been used to evaluate results,which indicate that integrated wavelet-decomposition and ANFIS models improve the accuracy of SWL prediction and that the inputs of SLA and wind-shear velocity exhibit superior prediction capability compared to conventional SWL-only models.
基金National natural science foundation(No.81303243)the project of education department of Shanxi province(No.20JC012)+1 种基金the postgraduate innovation project of Shanxi university of traditional Chinese medicine(No.2020-6)the innovation team of Shanxi university of traditional Chinese medicine(No,2019-QN02)。
文摘Objective:To systematically evaluate the safety and effectiveness of Qishen Yiqi dripping pills combined with trimetazidine in the treatment of chronic heart failure.Methods:A total of four English and Chinese electronic databases were searched:CNKI,VIP,CBM,PUBMED.Cochrane bias risk tools were used to assess the methodological quality of qualified studies.Meta-analysis was conducted by Review Manager 5.3.A total of 9 articles were included,with a total sample size of 855 cases,443 cases in the observation group and 412 cases in the treatment group.Results:Qishen Yiqi dripping pills combined with trimetazidine in the treatment of chronic heart failure has a total effective rate(RR=1.22,95%CI[1.15-1.30];p<0.00001),LVEF(MD=6.03,95%CI[5.39,6.67],P<0.00001),BNP(MD=-101.87,95%CI[-109.90,-93.83],P<0.00001),E/A(MD=-4.32,95%CI[-5.70,-2.93],P<0.00001),SYP(MD=-10.32,95%CI[-13.32,-7.32],P<0.00001),LVESD(MD=-5.50,95%CI[-6.03,-4.96],P<0.00001),6-MWT(MD=110.13,95%CI[96.89,123.36],P<0.00001)Adverse reactions occurred less frequently and did not affect treatment.Conclusion:This study shows that QYDP combined with TMZ can improve various indicators of CHF patients.However,due to the small sample size and the generally low quality of research,a more rigorous and reasonably designed RCT is needed to confirm these findings.
文摘Objective:To investigate the effect of dexmedetomidine combined with ultrasound guided nerve block on the hemodynamics, immune function and cortisol level in the patients undergoing inguinal surgery.Methods:A total of 120 patients who underwent unilateral inguinal surgery in our hospital were randomly divided into control group and observation group, 60 cases in each group. The control group was induced by intravenous anesthesia with sufentanil. The observation group was given dexmedetomidine combined with ultrasound guided ilioinguinal/iliac hypogastric nerve block. Hemodynamics, immune function and cortisol levels were compared between the two groups before and after anesthesia induction. Results:After anesthesia, in surgical incision and recovery time, the levels of HR in the two groups were significantly lower than that before the anesthesia, and the levels of HR in the observation group were significantly lower than that in the control group. After anesthesia, in surgical incision and recovery time, the levels of CD3+, CD4+ and CD4+/CD8+ in the control group were significantly lower than that of the group before anesthesia. After anesthesia, the levels of CD3+, CD4+ and CD4+/CD8+ in the observation group were significantly lower than that of the group before anesthesia. In surgical incision and recovery time, there was no significant difference in the levels of CD3+, CD4+ and CD4+/CD8+ in the observation group before anesthesia. After anesthesia, in surgical incision and recovery time, the levels of CD3+, CD4+ and CD4+/CD8+ in the observation group were significantly higher than that of the control group. The levels of serum PI3K, ET -1, CRP and cortisol of the two groups were significantly higher than those before anesthesia, and the levels of PI3K, ET -1, CRP and cortisol in the serum of the patients in the observation group were significantly lower than those in the control group.Conclusion: The effect of dexmedetomidin combined with ultrasound guided nerve block anesthesia on the patients with inguinal surgery can effectively maintain the hemodynamic stability of the patients and have less influence on the immune function and the stress response. The security is higher.
基金sponsored by the National Natural Science Foundation of China(Grant Nos.41930971,42105061 and 42030604).
文摘This article summarizes the progress made in predictability studies of weather and climate in recent years in China,with a main focus on advances in methods to study error growth dynamics and reduce uncertainties in the forecasting of weather and climate.Specifically,it covers(a)advances in methods to study weather and climate predictability dynamics,especially those in nonlinear optimal perturbation methods associated with initial errors and model errors and their applications to ensemble forecasting and target observations,(b)new data assimilation algorithms for initialization of predictions and novel assimilation approaches to neutralize the combined effects of initial and model errors for weather and climate,(c)applications of new statistical approaches to climate predictions,and(d)studies on meso-to small-scale weather system predictability dynamics.Some of the major frontiers and challenges remaining in predictability studies are addressed in this context.
