Low-affinity nitrate transporter genes have been identified in subfamilies 4-8 of the rice nitrate transporter 1(NRT1)/peptide transporter family(NPF),but the OsNPF3 subfamily responsible for nitrate and phytohormone ...Low-affinity nitrate transporter genes have been identified in subfamilies 4-8 of the rice nitrate transporter 1(NRT1)/peptide transporter family(NPF),but the OsNPF3 subfamily responsible for nitrate and phytohormone transport and rice growth and development remains unknown.In this study,we described OsNPF3.1 as an essential nitrate and phytohormone transporter gene for rice tillering and nitrogen utilization efficiency(NUtE).OsNPF3.1 possesses four major haplotypes of its promoter sequence in 517 cultivars,and its expression is positively associated with tiller number.Its expression was higher in the basal part,culm,and leaf blade than in other parts of the plant,and was strongly induced by nitrate,abscisic acid(ABA)and gibberellin 3(GA_3)in the root and shoot of rice.Electrophysiological experiments demonstrated that OsNPF3.1 is a pH-dependent low-affinity nitrate transporter,with rice protoplast uptake assays showing it to be an ABA and GA_3 transporter.OsNPF3.1 overexpression significantly promoted ABA accumulation in the roots and GA accumulation in the basal part of the plant which inhibited axillary bud outgrowth and rice tillering,especially at high nitrate concentrations.The NUtE of OsNPF3.1-overexpressing plants was enhanced under low and medium nitrate concentrations,whereas the NUtE of OsNPF3.1 clustered regularly interspaced short palindromic repeats(CRISPR)plants was increased under high nitrate concentrations.The results indicate that OsNPF3.1 transports nitrate and phytohormones in different rice tissues under different nitrate concentrations.The altered OsNPF3.1 expression improves NUtE in the OsNPF3.1-overexpressing and CRISPR lines at low and high nitrate concentrations,respectively.展开更多
Pre-driven longwall retracement roadway(PLRR)is commonly used in large mine shaft.The support crushing disasters occur frequently during the retracement,and roof management is necessary.Taking the 31107 panel as resea...Pre-driven longwall retracement roadway(PLRR)is commonly used in large mine shaft.The support crushing disasters occur frequently during the retracement,and roof management is necessary.Taking the 31107 panel as research background,the roof breaking structure of PLRR is analyzed.It is concluded that the roof cutting with vertical hydraulic fracture(HF)at a specified position,that is,fixed-length roof cutting,can reduce support load and keep immediate roof intact.The extended finite element method(XFEM)is applied to simulate hydraulic fracturing.The results show that both the axial and transverse hydraulic fracturing cannot effectively create vertical HFs.Therefore,a novel construction method of vertical HF based on the stress shadow effect(SSE)is proposed.The stress reversal region and HF orientation caused by the prefabricated hydraulic fracture(PF)are verified in simulation.The sub-vertical HFs are obtained between two PFs,the vertical extension range of which is much larger than that of directional hydraulic fracturing.The new construction method was used to determine the field plan for fixed-length roof cutting.The roof formed a stable suspended structure and deformation of the main PLRR was improved after hydraulic fracturing.展开更多
1. Introduction The increasing global demand for sustainable energy sources and emerging environmental issues have pushed the development of energy conversion and storage technologies to the forefront of chemical rese...1. Introduction The increasing global demand for sustainable energy sources and emerging environmental issues have pushed the development of energy conversion and storage technologies to the forefront of chemical research [1,2]. In particular, electrochemical CO_(2) reduction(CO_(2) R) to value-added fuels and chemicals presents a feasible pathway for renewable energy storage and could help mitigate the ever-increasing CO_(2) emissions [3].展开更多
Ultra-deep desulfurization of transformer oil is of great demand among power industry.In this work,the effective and deep removal of various types of organosulfurs,including mercaptan,sulfide and disulfide via catalyt...Ultra-deep desulfurization of transformer oil is of great demand among power industry.In this work,the effective and deep removal of various types of organosulfurs,including mercaptan,sulfide and disulfide via catalytic adsorptive desulfurization(CADS)using bifunctional Ti-based adsorbent is reported.Compared to adsorptive desulfurization(ADS),dramatically improvement of the organosulfur uptakes were achieved under CADS process.The equilibrium adsorption capacity at 5 μg∙g^(-1) S reached up to 15.7,33.4,11.6 and 11.9 mg∙g^(-1) for propyl mercaptan(n-PM),dimethyl sulfide(DMS),di-t-butyl disulfide(DTBDS)and dibenzyl disulfide(DBDS),which was 262,477,97 and 128 times to that of ADS process,respectively,and was the highest among the reported desulfurization adsorbents.Moreover,it achieved superior breakthrough capacity of 2050,530 and 210 ml F·(g A)^(-1) at the breakthrough S concentration of 1 μg∙g^(-1) of the commercial transformer oil S containing 10,50 and 150 μg∙g^(-1),respectively.The effectiveness of CADS is associated to the transformation of sulfur species to higher polar sulfonic species with the assistance of mild oxidant,which can be readily captured by silanol groups on SiO2 through Hbonding interaction.The excellent recyclability of the adsorbent can be realized through solvent washing or oxidative air treatment.This work provides an effective and economic approach for the elimination of trace amount of mercaptan,sulfide and disulfide from transformer oil.展开更多
Gastric cancer(GC), the fifth most common cancer globally, remains the leading cause of cancer deaths worldwide. Inflammation-induced tumorigenesis is the predominant process in GC development;therefore, systematic re...Gastric cancer(GC), the fifth most common cancer globally, remains the leading cause of cancer deaths worldwide. Inflammation-induced tumorigenesis is the predominant process in GC development;therefore, systematic research in this area should improve understanding of the biological mechanisms that initiate GC development and promote cancer hallmarks. Here, we summarize biological knowledge regarding gastric inflammation-induced tumorigenesis, and characterize the multi-omics data and systems biology methods for investigating GC development. Of note, we highlight pioneering studies in multi-omics data and state-of-the-art network-based algorithms used for dissecting the features of gastric inflammation-induced tumorigenesis, and we propose translational applications in early GC warning biomarkers and precise treatment strategies. This review offers integrative insights for GC research, with the goal of paving the way to novel paradigms for GC precision oncology and prevention.展开更多
A palladium-catalyzed regioselective three-component cascade reaction of carbon dioxide,amines and allenes has been developed,providing an expedient and practical method for the construction of a range of functionaliz...A palladium-catalyzed regioselective three-component cascade reaction of carbon dioxide,amines and allenes has been developed,providing an expedient and practical method for the construction of a range of functionalized carbamates containing dihydrobenzofuran or indole moiety in moderate to excellent yields.The broad substrate scope,good functional group tolerance and excellent chemo-and regioselectivity are the features of the transformation.展开更多
Recently,a paper published in Science by Zheng et al.reported that CD8αwas crucial to physiologically maintain the quiescent state of CD8^(+)T cells in peripheral lymphoid organs.Paired immunoglobulin-like type 2 rec...Recently,a paper published in Science by Zheng et al.reported that CD8αwas crucial to physiologically maintain the quiescent state of CD8^(+)T cells in peripheral lymphoid organs.Paired immunoglobulin-like type 2 receptor alpha(PILRα)was recognized as the ligand for CD8αand blockade of PILRα-CD8αinteraction would disrupt CD8^(+)T-cell quiescence.展开更多
基金supported by the the Guizhou Provincial Excellent Young Talents Project of Science and Technology,China(YQK(2023)002)the Guizhou Provincial Science and Technology Projects,China((2022)Key 008)+2 种基金the Guizhou Provincial Science and Technology Support Plan,China((2022)Key 026)the Key Laboratory of Molecular Breeding for Grain and Oil Crops in Guizhou Province,China((2023)008)the Key Laboratory of Functional Agriculture of Guizhou Provincial Higher Education Institutions,China((2023)007)。
文摘Low-affinity nitrate transporter genes have been identified in subfamilies 4-8 of the rice nitrate transporter 1(NRT1)/peptide transporter family(NPF),but the OsNPF3 subfamily responsible for nitrate and phytohormone transport and rice growth and development remains unknown.In this study,we described OsNPF3.1 as an essential nitrate and phytohormone transporter gene for rice tillering and nitrogen utilization efficiency(NUtE).OsNPF3.1 possesses four major haplotypes of its promoter sequence in 517 cultivars,and its expression is positively associated with tiller number.Its expression was higher in the basal part,culm,and leaf blade than in other parts of the plant,and was strongly induced by nitrate,abscisic acid(ABA)and gibberellin 3(GA_3)in the root and shoot of rice.Electrophysiological experiments demonstrated that OsNPF3.1 is a pH-dependent low-affinity nitrate transporter,with rice protoplast uptake assays showing it to be an ABA and GA_3 transporter.OsNPF3.1 overexpression significantly promoted ABA accumulation in the roots and GA accumulation in the basal part of the plant which inhibited axillary bud outgrowth and rice tillering,especially at high nitrate concentrations.The NUtE of OsNPF3.1-overexpressing plants was enhanced under low and medium nitrate concentrations,whereas the NUtE of OsNPF3.1 clustered regularly interspaced short palindromic repeats(CRISPR)plants was increased under high nitrate concentrations.The results indicate that OsNPF3.1 transports nitrate and phytohormones in different rice tissues under different nitrate concentrations.The altered OsNPF3.1 expression improves NUtE in the OsNPF3.1-overexpressing and CRISPR lines at low and high nitrate concentrations,respectively.
基金financially supported by the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX21_2358)the National Key Research and Development Program of China(2020YFB1314204)National Natural Science Foundation of China(No.52074239)。
文摘Pre-driven longwall retracement roadway(PLRR)is commonly used in large mine shaft.The support crushing disasters occur frequently during the retracement,and roof management is necessary.Taking the 31107 panel as research background,the roof breaking structure of PLRR is analyzed.It is concluded that the roof cutting with vertical hydraulic fracture(HF)at a specified position,that is,fixed-length roof cutting,can reduce support load and keep immediate roof intact.The extended finite element method(XFEM)is applied to simulate hydraulic fracturing.The results show that both the axial and transverse hydraulic fracturing cannot effectively create vertical HFs.Therefore,a novel construction method of vertical HF based on the stress shadow effect(SSE)is proposed.The stress reversal region and HF orientation caused by the prefabricated hydraulic fracture(PF)are verified in simulation.The sub-vertical HFs are obtained between two PFs,the vertical extension range of which is much larger than that of directional hydraulic fracturing.The new construction method was used to determine the field plan for fixed-length roof cutting.The roof formed a stable suspended structure and deformation of the main PLRR was improved after hydraulic fracturing.
基金supported by the National Natural Science Foundation of China(Grants 21872039,22072030 and 52025023)the Science and Technology Commission of Shanghai Municipality(Grants 18JC1411700 and 19DZ2270100)+3 种基金the Key R&D Program of Guangdong Province(Grant 2020B010189001)funding support from the original personalized project of Fudan Universityfunding support from China Postdoctoral Science Foundation(2021M700810)。
文摘1. Introduction The increasing global demand for sustainable energy sources and emerging environmental issues have pushed the development of energy conversion and storage technologies to the forefront of chemical research [1,2]. In particular, electrochemical CO_(2) reduction(CO_(2) R) to value-added fuels and chemicals presents a feasible pathway for renewable energy storage and could help mitigate the ever-increasing CO_(2) emissions [3].
基金provided by the National Natural Science Foundation of China (22022806, 21776097)Natural Science Foundation of Guangdong Province (2017A030312005)
文摘Ultra-deep desulfurization of transformer oil is of great demand among power industry.In this work,the effective and deep removal of various types of organosulfurs,including mercaptan,sulfide and disulfide via catalytic adsorptive desulfurization(CADS)using bifunctional Ti-based adsorbent is reported.Compared to adsorptive desulfurization(ADS),dramatically improvement of the organosulfur uptakes were achieved under CADS process.The equilibrium adsorption capacity at 5 μg∙g^(-1) S reached up to 15.7,33.4,11.6 and 11.9 mg∙g^(-1) for propyl mercaptan(n-PM),dimethyl sulfide(DMS),di-t-butyl disulfide(DTBDS)and dibenzyl disulfide(DBDS),which was 262,477,97 and 128 times to that of ADS process,respectively,and was the highest among the reported desulfurization adsorbents.Moreover,it achieved superior breakthrough capacity of 2050,530 and 210 ml F·(g A)^(-1) at the breakthrough S concentration of 1 μg∙g^(-1) of the commercial transformer oil S containing 10,50 and 150 μg∙g^(-1),respectively.The effectiveness of CADS is associated to the transformation of sulfur species to higher polar sulfonic species with the assistance of mild oxidant,which can be readily captured by silanol groups on SiO2 through Hbonding interaction.The excellent recyclability of the adsorbent can be realized through solvent washing or oxidative air treatment.This work provides an effective and economic approach for the elimination of trace amount of mercaptan,sulfide and disulfide from transformer oil.
基金supported by funds from the National Natural Science Foundation of China (Grant No. T2341008)。
文摘Gastric cancer(GC), the fifth most common cancer globally, remains the leading cause of cancer deaths worldwide. Inflammation-induced tumorigenesis is the predominant process in GC development;therefore, systematic research in this area should improve understanding of the biological mechanisms that initiate GC development and promote cancer hallmarks. Here, we summarize biological knowledge regarding gastric inflammation-induced tumorigenesis, and characterize the multi-omics data and systems biology methods for investigating GC development. Of note, we highlight pioneering studies in multi-omics data and state-of-the-art network-based algorithms used for dissecting the features of gastric inflammation-induced tumorigenesis, and we propose translational applications in early GC warning biomarkers and precise treatment strategies. This review offers integrative insights for GC research, with the goal of paving the way to novel paradigms for GC precision oncology and prevention.
基金supported by the Ministry of Science and Technology of China(2016YFA0602900)the National Natural Science Foundation of China(21971073,21572071)+2 种基金the Guangdong Natural Science Foundation(2019A1515011468,2017A030313054)China Postdoctoral Science Foundation(2018M640774)the Fundamental Research Funds for the Central Universities(D2191970).
文摘A palladium-catalyzed regioselective three-component cascade reaction of carbon dioxide,amines and allenes has been developed,providing an expedient and practical method for the construction of a range of functionalized carbamates containing dihydrobenzofuran or indole moiety in moderate to excellent yields.The broad substrate scope,good functional group tolerance and excellent chemo-and regioselectivity are the features of the transformation.
基金supported by the Chinese National Natural Science Funds(81902947).
文摘Recently,a paper published in Science by Zheng et al.reported that CD8αwas crucial to physiologically maintain the quiescent state of CD8^(+)T cells in peripheral lymphoid organs.Paired immunoglobulin-like type 2 receptor alpha(PILRα)was recognized as the ligand for CD8αand blockade of PILRα-CD8αinteraction would disrupt CD8^(+)T-cell quiescence.
