Polyurethane(PU)ureteral stents are used in clinics to maintain the ureteral patency.Due to biofilm formation and encrustation complications,long-term clinical usage has been limited.It is therefore necessary to devel...Polyurethane(PU)ureteral stents are used in clinics to maintain the ureteral patency.Due to biofilm formation and encrustation complications,long-term clinical usage has been limited.It is therefore necessary to develop an effective response to this unmet medical need.A heparin/poly-L-lysine/copper(NPs)coating was developed in our previous work that showed the effect of preventing infection and encrustation in vitro.In this work,a further study was conducted by grafting NPs on clinical ureteral stents that then were implanted into the infectious bladders of Wistar rats to investigate the effects of nanoparticles on bacterial growth and crystal deposition in vivo.It was found that decreased numbers of adherent microbes,urease amount splitting by bacteria,and deposited crystals were observed on the NPs stents with significant differences in comparison with PU stents.Besides,histological analysis showed that the NPs stents decreased the host tissue inflammation in close relation to the decrease biofilm formation and encrustation after 28 days of implantation.展开更多
Ureteral stents are commonly utilized as a medical device to aid the flow of urine.However,biofilm formation and encrustation complications have been clinical problems.To overcome this challenge,heparin/poly-L-lysine-...Ureteral stents are commonly utilized as a medical device to aid the flow of urine.However,biofilm formation and encrustation complications have been clinical problems.To overcome this challenge,heparin/poly-L-lysine-copper(Hep/PLL-Cu)nanoparticle was immobilized on a dopamine-coated polyurethane surface(PU/NPs).The stability and structural properties of the nanoparticles were characterized by Zeta potential,poly dispersion index,transmission electron microscopy,atom force microscopy and contact angle.The surface composition,antibacterial potency,encrustation resistance rate and biocompatibility of PU/NPs were investigated by scanning electron microscope,X-ray photoelectron spectroscopy,antibacterial assay and MTS assay,respectively.In addition,the antiencrustation property was studied by implanting coated NPs stents in the rat bladder for 7 days.It was shown that the size and distribution of Hep/PLL-Cu nanoparticles were uniform.PU/NPs could inhibit Proteus mirabilis proliferation and biofilm formation,and exhibit no cytotoxicity.Less encrustation(Ca and Mg salt)was deposited both in vitro and in vivo on samples,demonstrating that the NPs coating could be a potential surface modification method of ureteral material for clinical use.展开更多
基金Liaoning Science and Technology Program(grant No.2020JH2/10300159).
文摘Polyurethane(PU)ureteral stents are used in clinics to maintain the ureteral patency.Due to biofilm formation and encrustation complications,long-term clinical usage has been limited.It is therefore necessary to develop an effective response to this unmet medical need.A heparin/poly-L-lysine/copper(NPs)coating was developed in our previous work that showed the effect of preventing infection and encrustation in vitro.In this work,a further study was conducted by grafting NPs on clinical ureteral stents that then were implanted into the infectious bladders of Wistar rats to investigate the effects of nanoparticles on bacterial growth and crystal deposition in vivo.It was found that decreased numbers of adherent microbes,urease amount splitting by bacteria,and deposited crystals were observed on the NPs stents with significant differences in comparison with PU stents.Besides,histological analysis showed that the NPs stents decreased the host tissue inflammation in close relation to the decrease biofilm formation and encrustation after 28 days of implantation.
基金Liaoning Science and Technology Program(grant No.2020JH2/10300159).
文摘Ureteral stents are commonly utilized as a medical device to aid the flow of urine.However,biofilm formation and encrustation complications have been clinical problems.To overcome this challenge,heparin/poly-L-lysine-copper(Hep/PLL-Cu)nanoparticle was immobilized on a dopamine-coated polyurethane surface(PU/NPs).The stability and structural properties of the nanoparticles were characterized by Zeta potential,poly dispersion index,transmission electron microscopy,atom force microscopy and contact angle.The surface composition,antibacterial potency,encrustation resistance rate and biocompatibility of PU/NPs were investigated by scanning electron microscope,X-ray photoelectron spectroscopy,antibacterial assay and MTS assay,respectively.In addition,the antiencrustation property was studied by implanting coated NPs stents in the rat bladder for 7 days.It was shown that the size and distribution of Hep/PLL-Cu nanoparticles were uniform.PU/NPs could inhibit Proteus mirabilis proliferation and biofilm formation,and exhibit no cytotoxicity.Less encrustation(Ca and Mg salt)was deposited both in vitro and in vivo on samples,demonstrating that the NPs coating could be a potential surface modification method of ureteral material for clinical use.
