<span style="font-family:Verdana;">Globally dental diseases are mainly caused by</span><i><span style="font-family:Verdana;"> Streptococcus mutans</span></i><...<span style="font-family:Verdana;">Globally dental diseases are mainly caused by</span><i><span style="font-family:Verdana;"> Streptococcus mutans</span></i><span style="font-family:""><span style="font-family:Verdana;">, it is one of the leading causative agents of dental caries worldwide, because of its resistance to </span><span><span style="font-family:Verdana;">conventional antibacterial agents, alternative therapies are used to control resistance of oral pathogens. This research was done to determine the antibacterial activities of </span><i><span style="font-family:Verdana;">Psidium guajava</span></i><span style="font-family:Verdana;"> (guava) and </span><i><span style="font-family:Verdana;">Velvet tamarin</span></i><span style="font-family:Verdana;"> (Icheku) chewing sticks on </span><i><span style="font-family:Verdana;">Streptococcus mutans</span></i><span style="font-family:Verdana;"> isolated from the oral cavity. The study was conducted in Owerri Imo State Nigeria during November-December</span></span><span style="font-family:Verdana;"> period. Phytochemical analysis of the plant extracts was done using appropriate techniques. The procedure used for antimicrobial susceptibility test was disk diffusion method. Serial dilutions of</span><i><span style="font-family:Verdana;"> Psidium guajava</span></i><span style="font-family:Verdana;"> (guava) and </span><i><span style="font-family:Verdana;">Velvet tamarind</span></i><span style="font-family:Verdana;"> (Icheku) extracts were prepared, Muller-Hinton media was used to put together the extract of serial dilutions of </span><i><span style="font-family:Verdana;">Psidium guajava</span></i><span style="font-family:Verdana;"> (guava) and </span><i><span style="font-family:Verdana;">Velvet tamarin</span></i><span style="font-family:Verdana;"> (Icheku) and </span></span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">microbiological procedure w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> used for visually determining the minimum inhibitory concentration as well as minimum bactericidal concentration. Phytochemical evaluation of the plants</span><span style="font-family:Verdana;">’</span><span style="font-family:""><span style="font-family:Verdana;"> extracts revealed that it contains saponins, tannins, alkaloid, steriods, glycosides and phenol. The results obtained from the antibacterial susceptibility testing of the extracts against </span><i><span style="font-family:Verdana;">Streptococcus mutans</span></i><span style="font-family:Verdana;"> showed that the zones of inhibition recorded ranged from 18</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">mm to 27</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">mm. Ethanol (Soxhlet) extract of I</span><span style="font-family:Verdana;">cheku twig showed no zone of inhibition on the isolated organism. The</span><span style="font-family:Verdana;"> ethanol (soxhlet) extract of the individual </span><i><span style="font-family:Verdana;">Psidium guajava</span></i><span style="font-family:Verdana;"> (guava) and </span><i><span style="font-family:Verdana;">Velvet tamarin</span></i><span style="font-family:Verdana;"> (Icheku) has a better antibacterial effect when compared to their aqueous extracts and combined forms. </span><i><span style="font-family:Verdana;">Psidium guajava</span></i><span style="font-family:Verdana;"> (Guava) and </span><i><span style="font-family:Verdana;">Velvet tamarin</span></i><span style="font-family:Verdana;"> (Icheku) twigs are made up of composite that </span></span><span style="font-family:Verdana;">is</span><span style="font-family:""><span style="font-family:Verdana;"> active against </span><i><span style="font-family:Verdana;">S. mutans</span></i><span style="font-family:Verdana;"> and can be used in oral hygiene. There is </span></span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">need for further investigation on the plant extracts as the rural poor make use of it because it is cheap, readily available and the rich also use it once they are in the village mostly in Eastern Nigeria. Similarly chewing sticks has been reported to be practiced by 90 of rural population in Nigeria</span><span style="font-family:Verdana;">.展开更多
Prevalence of cervical Human Papillomavirus infection and type distribution vary with different environments. Knowledge of this will help in some cervical cancer interventions such as vaccine administration. This stud...Prevalence of cervical Human Papillomavirus infection and type distribution vary with different environments. Knowledge of this will help in some cervical cancer interventions such as vaccine administration. This study aimed at determining the prevalence of cervical HPV infection among women attending a tertiary hospital in Awka, Nigeria. To evaluate cervical HPV Infections, cervical samples were collected over a period of 1 year from 405 consenting women. Multiplex PCR and cytology were used in the study. Semi-structured questionnaire was used to obtain the demographic characteristics of the participants. Statistical analysis was done using IBM SPSS statistics version 21. The findings showed that of the 405 women, 387 (94.4%) had normal cytology, and 18 (4.4%) had Low-grade Squamous Intraepithelial Lesion (LSIL). There was no High-grade Squamous Intraepithelial Lesion (HSIL). HPV prevalence of (79) 19.5% was obtained overall in the 405 women, (75 of 387) 19.4% with normal cytology, (4 of 18) 22.2% with LSIL. Age specific prevalence peaked at age group 30 - 39 and a second peak at 60 - 69. HPV types obtained were HPV 16 31 (7.7%), HPV 18 24 (5.9%), HPV 35 3 (0.7%), HPV 33 9 (2.2%), HPV 68 3 (0.7%) and multiple infections (9) 2.2%. HPV 16 was the only type found in LSIL. Regular HPV typing and screening of our women for HPV infection and Pap’s smear can go a long way in the reduction of cervical cancer.展开更多
文摘<span style="font-family:Verdana;">Globally dental diseases are mainly caused by</span><i><span style="font-family:Verdana;"> Streptococcus mutans</span></i><span style="font-family:""><span style="font-family:Verdana;">, it is one of the leading causative agents of dental caries worldwide, because of its resistance to </span><span><span style="font-family:Verdana;">conventional antibacterial agents, alternative therapies are used to control resistance of oral pathogens. This research was done to determine the antibacterial activities of </span><i><span style="font-family:Verdana;">Psidium guajava</span></i><span style="font-family:Verdana;"> (guava) and </span><i><span style="font-family:Verdana;">Velvet tamarin</span></i><span style="font-family:Verdana;"> (Icheku) chewing sticks on </span><i><span style="font-family:Verdana;">Streptococcus mutans</span></i><span style="font-family:Verdana;"> isolated from the oral cavity. The study was conducted in Owerri Imo State Nigeria during November-December</span></span><span style="font-family:Verdana;"> period. Phytochemical analysis of the plant extracts was done using appropriate techniques. The procedure used for antimicrobial susceptibility test was disk diffusion method. Serial dilutions of</span><i><span style="font-family:Verdana;"> Psidium guajava</span></i><span style="font-family:Verdana;"> (guava) and </span><i><span style="font-family:Verdana;">Velvet tamarind</span></i><span style="font-family:Verdana;"> (Icheku) extracts were prepared, Muller-Hinton media was used to put together the extract of serial dilutions of </span><i><span style="font-family:Verdana;">Psidium guajava</span></i><span style="font-family:Verdana;"> (guava) and </span><i><span style="font-family:Verdana;">Velvet tamarin</span></i><span style="font-family:Verdana;"> (Icheku) and </span></span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">microbiological procedure w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> used for visually determining the minimum inhibitory concentration as well as minimum bactericidal concentration. Phytochemical evaluation of the plants</span><span style="font-family:Verdana;">’</span><span style="font-family:""><span style="font-family:Verdana;"> extracts revealed that it contains saponins, tannins, alkaloid, steriods, glycosides and phenol. The results obtained from the antibacterial susceptibility testing of the extracts against </span><i><span style="font-family:Verdana;">Streptococcus mutans</span></i><span style="font-family:Verdana;"> showed that the zones of inhibition recorded ranged from 18</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">mm to 27</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">mm. Ethanol (Soxhlet) extract of I</span><span style="font-family:Verdana;">cheku twig showed no zone of inhibition on the isolated organism. The</span><span style="font-family:Verdana;"> ethanol (soxhlet) extract of the individual </span><i><span style="font-family:Verdana;">Psidium guajava</span></i><span style="font-family:Verdana;"> (guava) and </span><i><span style="font-family:Verdana;">Velvet tamarin</span></i><span style="font-family:Verdana;"> (Icheku) has a better antibacterial effect when compared to their aqueous extracts and combined forms. </span><i><span style="font-family:Verdana;">Psidium guajava</span></i><span style="font-family:Verdana;"> (Guava) and </span><i><span style="font-family:Verdana;">Velvet tamarin</span></i><span style="font-family:Verdana;"> (Icheku) twigs are made up of composite that </span></span><span style="font-family:Verdana;">is</span><span style="font-family:""><span style="font-family:Verdana;"> active against </span><i><span style="font-family:Verdana;">S. mutans</span></i><span style="font-family:Verdana;"> and can be used in oral hygiene. There is </span></span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">need for further investigation on the plant extracts as the rural poor make use of it because it is cheap, readily available and the rich also use it once they are in the village mostly in Eastern Nigeria. Similarly chewing sticks has been reported to be practiced by 90 of rural population in Nigeria</span><span style="font-family:Verdana;">.
文摘Prevalence of cervical Human Papillomavirus infection and type distribution vary with different environments. Knowledge of this will help in some cervical cancer interventions such as vaccine administration. This study aimed at determining the prevalence of cervical HPV infection among women attending a tertiary hospital in Awka, Nigeria. To evaluate cervical HPV Infections, cervical samples were collected over a period of 1 year from 405 consenting women. Multiplex PCR and cytology were used in the study. Semi-structured questionnaire was used to obtain the demographic characteristics of the participants. Statistical analysis was done using IBM SPSS statistics version 21. The findings showed that of the 405 women, 387 (94.4%) had normal cytology, and 18 (4.4%) had Low-grade Squamous Intraepithelial Lesion (LSIL). There was no High-grade Squamous Intraepithelial Lesion (HSIL). HPV prevalence of (79) 19.5% was obtained overall in the 405 women, (75 of 387) 19.4% with normal cytology, (4 of 18) 22.2% with LSIL. Age specific prevalence peaked at age group 30 - 39 and a second peak at 60 - 69. HPV types obtained were HPV 16 31 (7.7%), HPV 18 24 (5.9%), HPV 35 3 (0.7%), HPV 33 9 (2.2%), HPV 68 3 (0.7%) and multiple infections (9) 2.2%. HPV 16 was the only type found in LSIL. Regular HPV typing and screening of our women for HPV infection and Pap’s smear can go a long way in the reduction of cervical cancer.
